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DCK antibody :: deoxycytidine kinase (DCK), mRNA [Monoclonal Antibody]
Catalog #    MBS120196
Unit / Price
0.1 mg  /  $345.00 +1 FREE 8GB USB
    
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    deoxycytidine kinase (DCK), mRNA, Antibody
 Also Known As    Mouse monoclonal antibody Anti-Human DCK
 Product Synonym Names    Homo sapiens deoxycytidine kinase (DCK), mRNA
 Product Gene Name    DCK antibody [Similar Products]
 Product Synonym Gene Name    MGC117410; MGC138632 [Similar Products]
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 Immunogen Sequence Length    260
 Chromosome Location    Chromosome: 4; NC_000004.11 (71859265..71896631). Location: 4q13.3-q21.1
 OMIM    125450
 3D Structure    ModBase 3D Structure for P27707
 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    2243C2
 Host    Mouse
 Species Reactivity    Human
 Purity/Purification    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
 Form/Format    In PBS (3.0 mM KCl, 1.5 mM KH2PO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
 Concentration    100 ug/ml (1.0 ml) (lot specific)
 Sterility    Filtered through a 0.22 um membrane.
 Molecular Weight of Tag    approx. 20kDa
 Number of Amino Acids    98
 Preparation and Storage    Store at 2-8 degree C for up to one year. We recommend storing at -20 degree C for long-term storage. Avoid repeat freezing and thawing cycles.
 Other Notes for DCK antibody    Small volumes of DCK antibody may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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 Product Description specifically for DCK antibody    Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [NCBI Entrez Gene Summary]
 Applications Tested/Suitable for DCK antibody    Dot Blot
 Application Notes for DCK antibody    Primary Ab Dilution: 1:100
Secondary Ab Dilution: 1:3,000
(Sheep Anti Mouse IgG) Original Conc. Antibody: 0.63mg/ml
HRP: 0.78mg/ml

Immunogen amount (ng/lane): 25
 Quality Control for DCK antibody    Western blot analysis of immunized recombinant protein, using anti-DCK monoclonal antibody.
DCK antibody Quality Control image
 Quality Control #2 for DCK antibody    Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
DCK antibody Quality Control #2 image
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NCBI/Uniprot data below describe general gene information for DCK. It may not necessarily be applicable to this product.
 NCBI GI #    194578889 [Other Products]
 NCBI GeneID    1633 [Other Products]
 NCBI Accession #    NM_000788.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000788 [Other Products]
 UniProt Primary Accession #    P27707 [Other Products]
 UniProt Secondary Accession #    Q5TZY7; Q6FI11; B2R8V6 [Other Products]
 UniProt Related Accession #    P27707 [Other Products]
 Molecular Weight    30,519 Da
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 NCBI Official Full Name    Homo sapiens deoxycytidine kinase (DCK), mRNA
 NCBI Official Synonym Full Names    deoxycytidine kinase
 NCBI Official Symbol    DCK [Similar Products]
 NCBI Official Synonym Symbols    MGC117410; MGC138632 [Similar Products]
 NCBI Protein Information    deoxycytidine kinase; OTTHUMP00000160356; OTTHUMP00000219118; OTTHUMP00000219119; deoxynucleoside kinase
 UniProt Protein Name    Deoxycytidine kinase
 UniProt Gene Name    DCK [Similar Products]
 UniProt Entry Name    DCK_HUMAN
 NCBI Summary for DCK    Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq]
 UniProt Comments for DCK    Function: Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. Ref.16 Ref.19

Catalytic activity: NTP + deoxycytidine = NDP + dCMP. Ref.13 Ref.16 Ref.17 Ref.19

Subunit structure: Homodimer. Ref.13 Ref.14

Subcellular location: Nucleus Ref.8.

Post-translational modification: Phosphorylated upon DNA damage, probably by ATM or ATR

By similarity. Ref.9 Ref.10 Ref.11

Sequence similarities: Belongs to the DCK/DGK family.

Biophysicochemical propertiesKinetic parameters:KM=6.2 µM for deoxycytidine (dC) Ref.13KM=15.5 µM for cytarabine (araC)KM=22 µM for gemcitabine (dC)
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 NCBI References for DCK    1. Deoxycytidine kinase modulates the impact of the ABC transporter ABCG2 on clofarabine cytotoxicity.
2. Enhancement of gemcitabine sensitivity in pancreatic cancer by co-regulation of dCK and p8 expression.
3. Relationship between deoxycytidine kinase (DCK) genotypic variants and fludarabine toxicity in patients with follicular lymphoma.
4. Effect of genetic alterations of cytarabine- metabolizing enzymes in childhood acute lymphoblastic leukemia.
5. Gemcitabine metabolic and transporter gene polymorphisms are associated with drug toxicity and efficacy in patients with locally advanced pancreatic cancer.
 UniProt References for DCK    • Cloning and expression of human deoxycytidine kinase cDNA. Chottiner E.G., Shewach D.S., Datta N.S., Ashcraft E., Gribbin D., Ginsburg D., Fox I.H., Mitchell B.S.Proc. Natl. Acad. Sci. U.S.A. 88:1531-1535(1991) [PubMed: 1996353] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 58-70; 119-127 AND 189-192.
• Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
• Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201). Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
• Cloning of human full-length CDSs in BD Creator(TM) system donor vector. Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. [5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
 Research Articles on DCK    1. Overexpressed dCK and knocked down p8 is associated with enhancement of gemcitabine sensitivity in pancreatic cancer.
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 Searchable Name for DCK    anti-DCK; Buy DCK antibody :: deoxycytidine kinase (DCK), mRNA, Monoclonal Antibody (MBS120196) datasheet at MyBioSource; anti-DCK; MGC117410; MGC138632; MGC117410; MGC138632; anti-deoxycytidine kinase (DCK), mRNA; Mouse monoclonal antibody Anti-Human DCK; Homo sapiens deoxycytidine kinase (DCK), mRNA; Homo sapiens deoxycytidine kinase (DCK), mRNA; deoxycytidine kinase; Deoxycytidine kinase Mouse anti-Human. (Tested application: Dot Blot) (NCBI/UniProt 194578889 NM_000788.2 P27707 NM_000788 P27707 Q5TZY7; Q6FI11; B2R8V6 30,519 Da); IgG1; 2243C2; Chromosome: 4; NC_000004.11 (71859265..71896631). Location: 4q13.3-q21.1; 1633; DCK_HUMAN; DCK antibody; anti-DCK
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Pathways associated with DCK antibodyDiseases associated with DCK antibody
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 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >197 publications with DCK and Neoplasms
 Leukemia Antibodies  >129 publications with DCK and Leukemia
 Leukemia, Myeloid Antibodies  >49 publications with DCK and Leukemia, Myeloid
 Carcinoma Antibodies  >46 publications with DCK and Carcinoma
 Leukemia, Myeloid, Acute Antibodies  >45 publications with DCK and Leukemia, Myeloid, Acute
 Drug Toxicity Antibodies  >35 publications with DCK and Drug Toxicity
 Pancreatic Neoplasms Antibodies  >28 publications with DCK and Pancreatic Neoplasms
 Neoplasms, Experimental Antibodies  >25 publications with DCK and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >24 publications with DCK and Lung Neoplasms
 Lymphoma Antibodies  >19 publications with DCK and Lymphoma
Organs/Tissues associated with DCK antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >99 publications with DCK and Blood
 Lung Antibodies  >36 publications with DCK and Lung
 Brain Antibodies  >22 publications with DCK and Brain
 Bone Marrow Antibodies  >21 publications with DCK and Bone Marrow
 Liver Antibodies  >17 publications with DCK and Liver
 Spleen Antibodies  >15 publications with DCK and Spleen
 Lymph Node Antibodies  >8 publications with DCK and Lymph Node
 Lymph Antibodies  >8 publications with DCK and Lymph
 Thymus Antibodies  >7 publications with DCK and Thymus
 Pancreas Antibodies  >6 publications with DCK and Pancreas
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