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APOA1 protein :: APOLIPOPROTEIN A1 (APO A1), Human [Protein]
Catalog #    MBS173020
Unit / Price
0.1 mg  /  $155.00 + 1 FREE 8GB USB Flash Drive
0.5 mg  /  $290.00 + 1 FREE 8GB USB Flash Drive
1 mg  /  $375.00 + 1 FREE 8GB USB Flash Drive
3 mg  /  $615.00 + 1 FREE 8GB USB Flash Drive
    
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    APOLIPOPROTEIN A1 (APO A1), Human, Protein
 Also Known As    Human Plasma APOLIPOPROTEIN A1
 Product Synonym Names    Lipoproteins A1, apo A1
 Product Gene Name    APOA1 protein [Similar Products]
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 Immunogen Sequence Length    267
 Chromosome Location    Chromosome: 11; NC_000011.9 (116706469..116708338, complement). Location: 11q23-q24
 OMIM    105200
 3D Structure    ModBase 3D Structure for P02647
 Host    Human Serum
 Purity/Purification    > 96% by SDS-PAGE.
 Form/Format    Liquid buffer composed of 50mM Tris-HCL, 150mM NaCl, pH 7.4, 0.2 micron filtered.
 Concentration    0.9 mg/ml by Pierce BCA Protein Assay (BSA Standard) (lot specific)
 UNSPSC Code    51131909
 Protein    0.9 mg/ml (BCA)
 Preservatives    1.0 mg/ml sodium azide and 1.0 mg/ml disodium EDTA.
 Note    Product is soluble in aqueous buffers at low concentrations (<50 ug/mL), but due to amphipathic nature, it will self-associate into oligomers at high concentrations in the absence of chaostropes (urea, guanidine hudrochloride).
 Preparation and Storage    Store at -20 degree C long term. Short term store at 2 to 8 degree C. AVOID REPEAT FREEZE THAW CYCLES.
 Other Notes for APOA1 protein    Small volumes of APOA1 protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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 Product Description specifically for APOA1 protein    Manufacturer MyBioSource HUMAN APO A1 apolipoprotein(s) produced for research and bulk diagnostic manufacturing Custom Fills for Human APO A1 Lipoprotein available.

Human APO A1 apolipoprotein: Single polypeptide chain in 245 amino acids with high alpha-helical content, major structurual component of high density lipoproteins, cofactor for lecthin:cholesterol acyltransferase, levels in general are inversely related to risk of coronary artery disease in humans.

Human APO A1 apolipoprotein is isolated by ultracentrifugation and delipidated by organic solvents. Human APO A1 Lipoprotein is purified by ion-exchange chromatography.

Human APO A1 Lipoprotein is soluble in aqueous buffers at low concentrations (<50ug/mL) but due to amphipathic nature, Human APO A1 Lipoprotein will self-associate into oligomers at high concentrations in the absence of chaotrupes (urea, guanidine hydrochloride).

Our Human APO A1 apolipoprotein Sterile Filtered through a.22 micron hydrophilic filter.

Apo-AI comprises ~70% of the protein moiety in HDL. It is a single polypeptide chain consisting of 245 amino acids with glutamic acid as the C-terminal residue and aspartic acid as the N-terminal residue. The molecular mass is reported to be 28.3 kDa. The protein is made up of one major isoform (pI 5.6) and two minor isoforms (pI 5.53 and 5.46). Apo-AI shows a high content of -helix structure. The amphipathic regions in the -helix structure seem to be responsible for lipid binding capacity. In aqueous solution, Apo-AI shows self-association with minor conformation change. Apo-AI activates lecithin-cholesterol (LCAT) acyltransferase, which is responsible for cholesterol esterification in plasma.
 Product Categories/Family for APOA1 protein    High Purity Protein; Human Proteins
 Applications Tested/Suitable for APOA1 protein    ELISA, EIA, Radioimmunoassay (RIA)
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NCBI/Uniprot data below describe general gene information for APOA1. It may not necessarily be applicable to this product.
 NCBI GI #    4557321 [Other Products]
 NCBI GeneID    335 [Other Products]
 NCBI Accession #    NP_000030.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000039.1 [Other Products]
 UniProt Primary Accession #    P02647 [Other Products]
 UniProt Secondary Accession #    Q6LDN9; Q6Q785; Q9UCS8; Q9UCT8; A8K866 [Other Products]
 UniProt Related Accession #    P02647 [Other Products]
 Molecular Weight    28.3 kDa [Similar Products]
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 NCBI Official Full Name    apolipoprotein A-I preproprotein
 NCBI Official Synonym Full Names    apolipoprotein A-I
 NCBI Official Symbol    APOA1 [Similar Products]
 NCBI Protein Information    apolipoprotein A-I; apo-AI; apoA-I
 UniProt Protein Name    Apolipoprotein A-I
 UniProt Synonym Protein Names    Apolipoprotein A1Cleaved into the following chain:Truncated apolipoprotein A-I; Alternative name(s):; Apolipoprotein A-I(1-242)
 UniProt Gene Name    APOA1 [Similar Products]
 UniProt Synonym Gene Names    Apo-AI; ApoA-I [Similar Products]
 UniProt Entry Name    APOA1_HUMAN
 NCBI Summary for APOA1    This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]
 UniProt Comments for APOA1    Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Ref.20

Subunit structure: Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Ref.25 Ref.31 Ref.34

Subcellular location: Secreted.

Tissue specificity: Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Ref.32 Ref.35

Post-translational modification: Palmitoylated. Ref.28Met-110 and Met-136 are oxidized to methionine sulfoxides.Phosphorylation sites are present in the extracelllular medium.

Involvement in disease: Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [

MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant. Ref.39 Ref.40Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [

MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I. Ref.39 Ref.40Note=A mutation in APOA1 is the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA); also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed. Ref.39 Ref.40 Ref.45 Ref.46Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [

MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Ref.39 Ref.40 Ref.48

Sequence similarities: Belongs to the apolipoprotein A1/A4/E family.

Mass spectrometry: Molecular mass is 28081 Da from positions 25 - 267. Determined by ESI. Without methionine sulfoxide. Ref.32Molecular mass is 28098 Da from positions 25 - 267. Determined by ESI. With 1 methionine sulfoxide, oxidation at Met-110. Ref.32Molecular mass is 28095 Da from positions 25 - 267. Determined by ESI. With 1 methionine sulfoxide, oxidation at Met-136. Ref.32Molecular mass is 28114 Da from positions 25 - 267. Determined by ESI. With 2 methionine sulfoxides, oxidation at Met-110 and Met-136. Ref.32
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 NCBI References for APOA1    1. Low rate of production of apolipoproteins B100 and AI in 2 patients with Anderson disease (chylomicron retention disease).
2. Thermodynamics of protein self-association and unfolding. The case of apolipoprotein A-I.
3. Apo lipoprotein A1 gene polymorphisms predict cardio-metabolic risk in South Asian immigrants.
4. Genome-wide association study identifies multiple loci influencing human serum metabolite levels.
5. HNF4α increases liver-specific human ATP-binding cassette transporter A1 expression and cholesterol efflux to apolipoprotein A-I in response to cholesterol depletion.
 UniProt References for APOA1    • Gene structure of human apolipoprotein A1. Shoulders C.C., Kornblihtt A.R., Munro B.S., Baralle F.E.Nucleic Acids Res. 11:2827-2837(1983) [PubMed: 6406984] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
• Nucleotide sequence of cloned cDNA of human apolipoprotein A-I. Cheung P., Chan L.Nucleic Acids Res. 11:3703-3715(1983) [PubMed: 6304641] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
• Isolation and characterization of the human apolipoprotein A-I gene. Karathanasis S.K., Zannis V.I., Breslow J.L.Proc. Natl. Acad. Sci. U.S.A. 80:6147-6151(1983) [PubMed: 6413973] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
• Isolation and DNA sequence of full-length cDNA and of the entire gene for human apolipoprotein AI -- discovery of a new genetic polymorphism in the apo AI gene. Seilhamer J.J., Protter A.A., Frossard P., Levy-Wilson B.DNA 3:309-317(1984) [PubMed: 6207999] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
• Human apolipoproteins AI, AII, CII and CIII. cDNA sequences and mRNA abundance. Sharpe C.R., Sidoli A., Shelley C.S., Lucero M.A., Shoulders C.C., Baralle F.E.Nucleic Acids Res. 12:3917-3932(1984) [PubMed: 6328445] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
 Research Articles on APOA1    1. Six novel APOA1 gene single nucleotide polymorphisms associated with coronary artery disease in South Asians
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 Searchable Name for APOA1    anti-APOA1; Buy APOA1 protein :: APOLIPOPROTEIN A1 (APO A1), Human, Protein (MBS173020) datasheet at MyBioSource; anti-APOA1; Apo-AI; ApoA-I; anti-APOLIPOPROTEIN A1 (APO A1), Human; Human Plasma APOLIPOPROTEIN A1; Lipoproteins A1, apo A1; apolipoprotein A-I preproprotein; apolipoprotein A-I; Apolipoprotein A-I; Apolipoprotein A1Cleaved into the following chain:Truncated apolipoprotein A-I; Alternative name(s):; Apolipoprotein A-I(1-242) Human Serum . (Tested application: ELISA, EIA, Radioimmunoassay (RIA)) (NCBI/UniProt 4557321 NP_000030.1 P02647 NM_000039.1 P02647 Q6LDN9; Q6Q785; Q9UCS8; Q9UCT8; A8K866 28.3 kDa); High Purity Protein; Human Proteins; Chromosome: 11; NC_000011.9 (116706469..116708338, complement). Location: 11q23-q24; 335; APOA1_HUMAN; APOA1 protein; anti-APOA1
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with APOA1 proteinPathways associated with APOA1 protein
 Product Name  Ref Prod  PubMed Publications
 APOA1 protein  APOB protein  >373 publications with APOA1 and APOB
 APOA1 protein  ABCA1 protein  >90 publications with APOA1 and ABCA1
 APOA1 protein  CETP protein  >40 publications with APOA1 and CETP
 APOA1 protein  LCAT protein  >31 publications with APOA1 and LCAT
 APOA1 protein  SCARB1 protein  >21 publications with APOA1 and SCARB1
 APOA1 protein  PON1 protein  >15 publications with APOA1 and PON1
 APOA1 protein  PLTP protein  >11 publications with APOA1 and PLTP
 APOA1 protein  APOL1 protein  >3 publications with APOA1 and APOL1
 APOA1 protein  TTR protein  >2 publications with APOA1 and TTR
 APOA1 protein  ATP5B protein  >1 publications with APOA1 and ATP5B
 Product Name  Products by Pathway  Pathway Diagram
 APOA1 protein  ABC-family Proteins Mediated Transport Pathway antibodies  ABC-family Proteins Mediated Transport Pathway Diagram
 APOA1 protein  ABCA Transporters In Lipid Homeostasis Pathway antibodies  ABCA Transporters In Lipid Homeostasis Pathway Diagram
 APOA1 protein  African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 APOA1 protein  African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 APOA1 protein  Amyloids Pathway antibodies  Amyloids Pathway Diagram
 APOA1 protein  Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 APOA1 protein  Disease Pathway antibodies  Disease Pathway Diagram
 APOA1 protein  FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 APOA1 protein  Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 APOA1 protein  Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
Diseases associated with APOA1 proteinOrgans/Tissues associated with APOA1 protein
 Disease Name  Ref Prod  Pubmed Publications
 Cardiovascular Diseases Antibodies  anti-APOA1  >333 publications with APOA1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  anti-APOA1  >203 publications with APOA1 and Atherosclerosis
 Dyslipidemias Antibodies  anti-APOA1  >141 publications with APOA1 and Dyslipidemias
 Coronary Artery Disease Antibodies  anti-APOA1  >140 publications with APOA1 and Coronary Artery Disease
 Coronary Disease Antibodies  anti-APOA1  >140 publications with APOA1 and Coronary Disease
 Myocardial Ischemia Antibodies  anti-APOA1  >136 publications with APOA1 and Myocardial Ischemia
 Heart Diseases Antibodies  anti-APOA1  >134 publications with APOA1 and Heart Diseases
 Arteriosclerosis Antibodies  anti-APOA1  >132 publications with APOA1 and Arteriosclerosis
 Hyperlipidemias Antibodies  anti-APOA1  >101 publications with APOA1 and Hyperlipidemias
 Insulin Resistance Antibodies  anti-APOA1  >72 publications with APOA1 and Insulin Resistance
 Organ/Tissue Name  Ref Prod  Pubmed Publications
 Blood Antibodies  APOA1 antibody  >581 publications with APOA1 and Blood
 Heart Antibodies  APOA1 antibody  >121 publications with APOA1 and Heart
 Liver Antibodies  APOA1 antibody  >118 publications with APOA1 and Liver
 Muscle Antibodies  APOA1 antibody  >24 publications with APOA1 and Muscle
 Brain Antibodies  APOA1 antibody  >20 publications with APOA1 and Brain
 Ovary Antibodies  APOA1 antibody  >10 publications with APOA1 and Ovary
 Intestine Antibodies  APOA1 antibody  >7 publications with APOA1 and Intestine
 Lung Antibodies  APOA1 antibody  >5 publications with APOA1 and Lung
 Pancreas Antibodies  APOA1 antibody  >5 publications with APOA1 and Pancreas
 Embryonic Tissue Antibodies  APOA1 antibody  >3 publications with APOA1 and Embryonic Tissue
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