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CD142 antibody :: CD142 [Monoclonal Antibody]
Catalog #    MBS216245
Unit / Price
0.025 mg  /  $165.00 + 1 FREE 4GB USB Flash Drive
     
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    CD142 Antibody
 Also Known As    MOUSE ANTI HUMAN CD142:FITC
 Product Gene Name    CD142 antibody [Similar Products]
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 Immunogen Sequence Length    238
 Chromosome Location    Chromosome: 1; NC_000001.10 (94994732..95007413, complement). Location: 1p22-p21
 OMIM    134390
 3D Structure    ModBase 3D Structure for P13726
 Clonality    Monoclonal
 Isotype    IgG1
 Clone Number    CLB/TF-5
 Host    Mouse
 Species Reactivity    Human
 Specificity    CD142
This item recognises human CD142, a 45kD cell surface glycoprotein which is otherwise known as Tissue Factor (TF). CD142 expression can be induced on monocytes, macrophages and endothelial cells by various stimuli including interleukin 1, tumour necrosis factor and endotoxin. CD142 initiates the blood clotting cascade by binding coagulation factor VIIa, which activates factor IX or factor X by specific limited proteolysis. CD142 also plays an important role in inflammation, angiogenesis, and the pathophysiology of atherosclerosis and cancer.
 Form/Format    FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
 Concentration    IgG concentration 0.1mg/ml
 Preparation    Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
 Preservative Stabilizers    0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
 Buffer Solution    TRIS buffered saline
 Preparation and Storage    Store at +4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
 Other Notes for CD142 antibody    Small volumes of CD142 antibody may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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 Applications Tested/Suitable for CD142 antibody    Flow Cytometry (FACS)
 Application Notes for CD142 antibody    Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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NCBI/Uniprot data below describe general gene information for CD142. It may not necessarily be applicable to this product.
 NCBI GI #    296010912 [Other Products]
 NCBI GeneID    2152 [Other Products]
 NCBI Accession #    NP_001171567.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001178096.1 [Other Products]
 UniProt Primary Accession #    P13726 [Other Products]
 UniProt Secondary Accession #    Q6FHG2; Q86WH4; D3DT47 [Other Products]
 UniProt Related Accession #    P13726 [Other Products]
 Molecular Weight    33,068 Da
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 NCBI Official Full Name    tissue factor isoform 2
 NCBI Official Synonym Full Names    coagulation factor III (thromboplastin, tissue factor)
 NCBI Official Symbol    F3 [Similar Products]
 NCBI Official Synonym Symbols    TF; TFA; CD142 [Similar Products]
 NCBI Protein Information    tissue factor
 UniProt Protein Name    Tissue factor
 UniProt Synonym Protein Names    Coagulation factor III; Thromboplastin
 UniProt Gene Name    F3 [Similar Products]
 UniProt Synonym Gene Names    TF [Similar Products]
 UniProt Entry Name    TF_HUMAN
 NCBI Summary for CD142    This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
 UniProt Comments for CD142    Function: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Ref.6

Subunit structure: Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. Ref.15

Subcellular location: Isoform 1: Membrane; Single-pass type I membrane protein Ref.6. Isoform 2: Secreted Ref.6.

Tissue specificity: Lung, placenta and pancreas. Ref.6

Induction: TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Ref.14

Sequence similarities: Belongs to the tissue factor family.
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 NCBI References for CD142    1. [Tissue factor in endothelial cells--its structure and function according to the current literature].
2. Thioredoxin and thioredoxin reductase control tissue factor activity by thiol redox-dependent mechanism.
3. Cancer cells induced to express mesenchymal phenotype release exosome-like extracellular vesicles carrying tissue factor.
4. Tissue factor signals airway epithelial basal cell survival via coagulation and protease-activated receptor isoforms 1 and 2.
5. p21-activated kinase-1 signaling regulates transcription of tissue factor and tissue factor pathway inhibitor.
 UniProt References for CD142    • Human tissue factor: cDNA sequence and chromosome localization of the gene. Scarpati E.M., Wen D., Broze G.J. Jr., Miletich J.P., Flandermeyer R.R., Siegel N.R., Sadler J.E.Biochemistry 26:5234-5238(1987) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
• Molecular cloning of the cDNA for tissue factor, the cellular receptor for the initiation of the coagulation protease cascade. Morrissey J.H., Fakhrai H., Edgington T.S.Cell 50:129-135(1987) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
• Isolation of cDNA clones coding for human tissue factor: primary structure of the protein and cDNA. Spicer E.K., Horton R., Bloem L., Bach R., Williams K.R., Guha A., Kraus J., Lin T.C., Nemerson Y., Konigsberg W.H.Proc. Natl. Acad. Sci. U.S.A. 84:5148-5152(1987) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
• Cloning and expression of human tissue factor cDNA. Fisher K.L., Gorman C.M., Vehar G.A., O'Brien D.P., Lawn R.M.Thromb. Res. 48:89-99(1987) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
• Complete sequence of the human tissue factor gene, a highly regulated cellular receptor that initiates the coagulation protease cascade. Mackman N., Morrissey J.H., Fowler B., Edgington T.S.Biochemistry 28:1755-1762(1989) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
 Research Articles on CD142    1. study demonstrated for that TF regulates tumor angiogenesis of retinoblastoma; found that TF is expressed by endothelial cells of retinoblastoma, whose expression is upregulated with the proliferation of endothelial cells
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 Searchable Name for CD142    anti-CD142; Buy CD142 antibody :: CD142, Monoclonal Antibody (MBS216245) datasheet at MyBioSource; anti-CD142; TF; TFA; CD142; TF; anti-CD142; MOUSE ANTI HUMAN CD142:FITC; tissue factor isoform 2; coagulation factor III (thromboplastin, tissue factor); Tissue factor; Coagulation factor III; Thromboplastin Mouse anti-Human. (Tested application: Flow Cytometry (FACS)) (NCBI/UniProt 296010912 NP_001171567.1 P13726 NM_001178096.1 P13726 Q6FHG2; Q86WH4; D3DT47 33,068 Da); IgG1; CLB/TF-5; Chromosome: 1; NC_000001.10 (94994732..95007413, complement). Location: 1p22-p21; 2152; TF_HUMAN; CD142 antibody; anti-CD142
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with CD142 antibodyPathways associated with CD142 antibody
 Product Name  Ref Prod  PubMed Publications
 CD142 antibody  F10 antibody  >13 publications with CD142 and F10
 Product Name  Products by Pathway  Pathway Diagram
 CD142 antibody  Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 CD142 antibody  Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 CD142 antibody  Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 CD142 antibody  Extrinsic Pathway antibodies  Extrinsic Pathway Diagram
 CD142 antibody  Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 CD142 antibody  Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
Diseases associated with CD142 antibodyOrgans/Tissues associated with CD142 antibody
 Disease Name  Ref Prod  Pubmed Publications
 Neoplasms Antibodies  anti-CD142  >486 publications with CD142 and Neoplasms
 Fibrosis Antibodies  anti-CD142  >382 publications with CD142 and Fibrosis
 Leukemia Antibodies  anti-CD142  >305 publications with CD142 and Leukemia
 Carcinoma Antibodies  anti-CD142  >107 publications with CD142 and Carcinoma
 Inflammation Antibodies  anti-CD142  >70 publications with CD142 and Inflammation
 Lymphoma Antibodies  anti-CD142  >64 publications with CD142 and Lymphoma
 Neoplasms, Experimental Antibodies  anti-CD142  >61 publications with CD142 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  anti-CD142  >61 publications with CD142 and Carcinoma, Hepatocellular
 Fatty Liver Antibodies  anti-CD142  >59 publications with CD142 and Fatty Liver
 Adenocarcinoma Antibodies  anti-CD142  >50 publications with CD142 and Adenocarcinoma
 Organ/Tissue Name  Ref Prod  Pubmed Publications
 Brain Antibodies  CD142 antibody  >509 publications with CD142 and Brain
 Muscle Antibodies  CD142 antibody  >156 publications with CD142 and Muscle
 Ovary Antibodies  CD142 antibody  >151 publications with CD142 and Ovary
 Nerve Antibodies  CD142 antibody  >122 publications with CD142 and Nerve
 Kidney Antibodies  CD142 antibody  >107 publications with CD142 and Kidney
 Connective Tissue Antibodies  CD142 antibody  >104 publications with CD142 and Connective Tissue
 Vascular Antibodies  CD142 antibody  >99 publications with CD142 and Vascular
 Heart Antibodies  CD142 antibody  >90 publications with CD142 and Heart
 Eye Antibodies  CD142 antibody  >82 publications with CD142 and Eye
 Skin Antibodies  CD142 antibody  >66 publications with CD142 and Skin
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