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CD47 antibody :: CD47 Antigen (Rh-related Antigen, Integrin-associated Signal Transducer) (CD47) [Monoclonal Antibody]
Catalog #    MBS245160
Unit / Price
0.05 mg  /  $410.00 + 1 FREE 8GB USB Flash Drive
    
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    CD47 Antigen (Rh-related Antigen, Integrin-associated Signal Transducer) (CD47), Antibody
 Also Known As    CD47 Mouse anti-Human Monoclonal (HCD47) Antibody
 Product Synonym Names    CD47; IAP; Leukocyte surface antigen cd47; MER6; OA3
 Product Gene Name    CD47 antibody [Similar Products]
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 Chromosome Location    Chromosome: 3; NC_000003.11 (107761941..107809935, complement). Location: 3q13.1-q13.2
 OMIM    601028
 3D Structure    ModBase 3D Structure for Q08722
 Clonality    Monoclonal
 Isotype    IgG1, k
 Clone Number    HCD47
 Host    Mouse
 Species Reactivity    Human
 Purity/Purification    Protein G Column
 Form/Format    Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
 Concentration    0.5 mg/ml (lot specific)
 Antigen Species    CD47 antibody was raised against Human
 Preparation and Storage    Store at 4 degree C. Do not freeze.
 Other Notes for CD47 antibody    Small volumes of CD47 antibody may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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 Applications Tested/Suitable for CD47 antibody    Flow Cytometry, Immunohistochemistry - Paraffin
 Application Notes for CD47 antibody    Immunohistochemistry - Paraffin (IHC-P) (20 ug/ml), Flow Cytometry (Flo) (1 ug/1E6 cells) (Optimal dilution to be determined by the researcher)
Antibody Validation: It has been validated for use in the following assays: IHC.

Antibody Usage Summary: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for was determined to be 20 ug/ml.
 Immunohistochemistry for CD47 antibody    Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with CD47 antibody at 20 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
CD47 antibody Immunohistochemistry (IHC) image
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NCBI/Uniprot data below describe general gene information for CD47. It may not necessarily be applicable to this product.
 NCBI GI #    4502673 [Other Products]
 NCBI GeneID    961 [Other Products]
 NCBI Accession #    NP_001768.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001777.3 [Other Products]
 UniProt Primary Accession #    Q08722 [Other Products]
 UniProt Secondary Accession #    Q53Y71; Q96A60; A8K198; D3DN59 [Other Products]
 UniProt Related Accession #    Q08722; Q71A41 [Other Products]
 Molecular Weight    35,214 Da
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 NCBI Official Full Name    leukocyte surface antigen CD47 isoform 1
 NCBI Official Synonym Full Names    CD47 molecule
 NCBI Official Symbol    CD47 [Similar Products]
 NCBI Official Synonym Symbols    IAP; OA3; MER6 [Similar Products]
 NCBI Protein Information    leukocyte surface antigen CD47; CD47 glycoprotein; OTTHUMP00000041152; OTTHUMP00000041153; Rh-related antigen; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; antigenic surface determinant protein OA3; antigen identified by monoclonal antibody 1D8; CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
 UniProt Protein Name    Leukocyte surface antigen CD47
 UniProt Synonym Protein Names    Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6
 UniProt Gene Name    CD47 [Similar Products]
 UniProt Synonym Gene Names    MER6 [Similar Products]
 UniProt Entry Name    CD47_HUMAN
 NCBI Summary for CD47    This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
 UniProt Comments for CD47    Function: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus

By similarity. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. Ref.2 Ref.10 Ref.12

Subunit structure: Interacts with THBS1 and fibrinogen

By similarity. Monomer. Interacts with SIRPA, SIRPG, UBQLN1 and UBQLN2. Ref.8 Ref.10 Ref.12

Subcellular location: Cell membrane; Multi-pass membrane protein Ref.2.

Tissue specificity: Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.
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 NCBI References for CD47    1. Heparan sulfate modification of the transmembrane receptor CD47 is necessary for inhibition of T cell receptor signaling by thrombospondin-1.
2. The CD47 pathway is deregulated in human immune thrombocytopenia.
3. Amyloid-β inhibits No-cGMP signaling in a CD36- and CD47-dependent manner.
4. Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47.
5. Thrombospondin-1 inhibits VEGF receptor-2 signaling by disrupting its association with CD47.
 UniProt References for CD47    • An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains. Campbell I.G., Freemont P.S., Foulkes W., Trowsdale J.Cancer Res. 52:5416-5420(1992) [PubMed: 1394148] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM OA3-323). Tissue: Ovary.
• Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane spanning domains implicated in alpha-v beta-3-dependent ligand binding. Lindberg F.P., Gresham H.D., Schwarz E., Brown E.J.J. Cell Biol. 123:485-496(1993) [PubMed: 7691831] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM OA3-305), FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY. Tissue: Myelomonocyte.
• Cloning of human full-length CDSs in BD Creator(TM) system donor vector. Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM OA3-305).
• Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM OA3-323). Tissue: Brain. [5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
 Research Articles on CD47    1. inhibition of T cell receptor signaling by thrombospondin-1 is mediated by CD47 and requires its modification at Ser(64).
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 Searchable Name for CD47    anti-CD47; Buy CD47 antibody :: CD47 Antigen (Rh-related Antigen, Integrin-associated Signal Transducer) (CD47), Monoclonal Antibody (MBS245160) datasheet at MyBioSource; anti-CD47; IAP; OA3; MER6; MER6; anti-CD47 Antigen (Rh-related Antigen, Integrin-associated Signal Transducer) (CD47); CD47 Mouse anti-Human Monoclonal (HCD47) Antibody; CD47; IAP; Leukocyte surface antigen cd47; MER6; OA3; leukocyte surface antigen CD47 isoform 1; CD47 molecule; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6 Mouse anti-Human. (Tested application: Flow Cytometry, Immunohistochemistry - Paraffin) (NCBI/UniProt 4502673 NP_001768.1 Q08722; Q71A41 NM_001777.3 Q08722 Q53Y71; Q96A60; A8K198; D3DN59 35,214 Da); IgG1, k; HCD47; Chromosome: 3; NC_000003.11 (107761941..107809935, complement). Location: 3q13.1-q13.2; 961; CD47_HUMAN; CD47 antibody; anti-CD47
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with CD47 antibodyPathways associated with CD47 antibody
 Reference Product  PubMed Publications
 THBS1 antibody  >97 publications with CD47 and THBS1
 SIRPA antibody  >40 publications with CD47 and SIRPA
 RHAG antibody  >19 publications with CD47 and RHAG
 PTK2 antibody  >7 publications with CD47 and PTK2
 FAS antibody  >7 publications with CD47 and FAS
 Products by Pathway  Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Integrins In Angiogenesis Pathway antibodies  Integrins In Angiogenesis Pathway Diagram
 Interactions Of The Immunoglobulin Superfamily (IgSF) Member Proteins Pathway antibodies  Interactions Of The Immunoglobulin Superfamily (IgSF) Member Proteins Pathway Diagram
 Signal Regulatory Protein (SIRP) Family Interactions Pathway antibodies  Signal Regulatory Protein (SIRP) Family Interactions Pathway Diagram
Diseases associated with CD47 antibodyOrgans/Tissues associated with CD47 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >68 publications with CD47 and Neoplasms
 Inflammation Antibodies  >51 publications with CD47 and Inflammation
 Hematologic Diseases Antibodies  >48 publications with CD47 and Hematologic Diseases
 Autoimmune Diseases Antibodies  >31 publications with CD47 and Autoimmune Diseases
 Leukemia Antibodies  >29 publications with CD47 and Leukemia
 Genetic Diseases, Inborn Antibodies  >24 publications with CD47 and Genetic Diseases, Inborn
 Carcinoma Antibodies  >15 publications with CD47 and Carcinoma
 Neuroectodermal Tumors Antibodies  >14 publications with CD47 and Neuroectodermal Tumors
 Melanoma Antibodies  >13 publications with CD47 and Melanoma
 Leukemia, Myeloid Antibodies  >12 publications with CD47 and Leukemia, Myeloid
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >305 publications with CD47 and Blood
 Vascular Antibodies  >73 publications with CD47 and Vascular
 Bone Antibodies  >48 publications with CD47 and Bone
 Bone Marrow Antibodies  >40 publications with CD47 and Bone Marrow
 Muscle Antibodies  >40 publications with CD47 and Muscle
 Brain Antibodies  >36 publications with CD47 and Brain
 Skin Antibodies  >21 publications with CD47 and Skin
 Spleen Antibodies  >20 publications with CD47 and Spleen
 Lung Antibodies  >16 publications with CD47 and Lung
 Kidney Antibodies  >12 publications with CD47 and Kidney
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