View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List anti-AMPK-alpha (pT172) antibody :: Rabbit AMPK-alpha (pT172) Antibody Catalog # MBS462009 Unit / Price 0.1 mg / $330 +1 FREE 8GB USB 5x0.1 mg / $1,310 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name AMPK-alpha (pT172), Antibody Popular Item Full Product Name Rabbit anti Phospho- AMPK alpha 1 (pThr 172) Product Synonym Names Rabbit anti--AMPK alpha 1 (pT172) Phosphospecific; AMPKalpha-1; AMP-activated protein kinase alpha 1;PRKAA1; MGC57364; AMPK Product Gene Name anti-AMPK-alpha (pT172) antibody[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Host Rabbit Species Reactivity Human, Rat, Mouse, Bovine, Dog, Chicken, Dog, Insect Specificity This antibody recognizes AMPK alpha with a phosphorylated site of Thr 172. It does not cross-react with non-phosphospecific peptide. Purity/Purification The Rabbit IgG is purified by site-modified Epitope Affinity Purification. Form/Format This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer Antigen Preparation A synthetic peptide surrounding to the epitope -LRTSC- with a phosphorylation site at Thr 172 of AMPK alpha 1 protein from human origin. It is identical among human, mouse, rat, chicken, bovine, dog, and insect species. Positive Control Kidney Tissue Cellular Location Cell membrane Preparation and Storage The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C-8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles. Other Notes Small volumes of anti-AMPK-alpha (pT172) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-AMPK-alpha (pT172) antibody AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy balance at both the cellular and the whole body levels. Once activated, it affects a metabolic switch from an anabolic to a catabolic state, both by acutely phosphorylating metabolic enzymes and, in the longer term, by regulating gene expression. AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding APM to the beta domains triggers increased phosphorylation at Thr172 on the activation loop of the alpha subunit. AMP Phosphorylation at Thr172 is catalyzed by the tumor suppressor kinase LKB1 or CaMKK-beta, TGF-beta activated kinase-1 (TAK1). AMPKalpha is also phosphorylated at Thr258 and Ser485 (for alpha1; Ser491 for alpha2). Applications Tested/Suitable for anti-AMPK-alpha (pT172) antibody ELISA, Western Blot, Immunohistochemistry (IHC), Immunoprecipitation (IP) Application Notes for anti-AMPK-alpha (pT172) antibody Western Blot: 0.1-1 ug/mlELISA: 0.01-0.1 ug/mlImmunoprecipitation: 2-5 ug/mlOptimal dilutions should be determined by researchers for the specific applications. Testing Data of anti-AMPK-alpha (pT172) antibody Western Blot: The whole cell lysate derived from EGF stimulated A431 immunoblotted by Rabbit anti AMPK alpha 1 (pT172) antibody (Cat#MBS462009) at 1:500 (lane 2).BSA was loaded as a negative control (Lane 3). Observed a major immunoreactive band at molecular weight ~63kDa. TOP NCBI/Uniprot data below describe general gene information for AMPK-alpha (pT172). It may not necessarily be applicable to this product. NCBI GI # 60459958 TOP NCBI Official Full Name AMPK-alpha subunit TOP Product References and Citations for anti-AMPK-alpha (pT172) antibody Jing Chen et al. AMPK regulation of mouse oocyte meiotic resumption in vitro. Developmental Biology, 291 (2), 227-238 (2006). TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with anti-AMPK-alpha (pT172) antibody Reference Product PubMed Publications FLNA antibody >12 publications with AMPK-alpha (pT172) and FLNA Pathways associated with anti-AMPK-alpha (pT172) antibody Products by Pathway Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Organs/Tissues associated with anti-AMPK-alpha (pT172) antibody Organ/Tissue Name Pubmed Publications Bone Antibodies >13 publications with AMPK-alpha (pT172) and Bone Connective Tissue Antibodies >4 publications with AMPK-alpha (pT172) and Connective Tissue Heart Antibodies >3 publications with AMPK-alpha (pT172) and Heart Muscle Antibodies >3 publications with AMPK-alpha (pT172) and Muscle Brain Antibodies >3 publications with AMPK-alpha (pT172) and Brain Vascular Antibodies >2 publications with AMPK-alpha (pT172) and Vascular Embryonic Tissue Antibodies >2 publications with AMPK-alpha (pT172) and Embryonic Tissue Ear Antibodies >1 publications with AMPK-alpha (pT172) and Ear Kidney Antibodies >1 publications with AMPK-alpha (pT172) and Kidney Umbilical Cord Antibodies >1 publications with AMPK-alpha (pT172) and Umbilical Cord TOP