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OT elisa kit :: Rat oxytocin ELISA Kit

Catalog #    MBS700101 OT elisa kit
Unit / Price
48 Strip Wells  /  $540 +1 FREE 8GB USB
96 Strip Wells  /  $775 +1 FREE 8GB USB
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

oxytocin (OT), ELISA Kit

 Also Known As   

Rat oxytocin, OT ELISA Kit

 Product Synonym Names    Rat oxytocin; OT ELISA Kit; oxytocin
 Product Gene Name   

OT elisa kit

[Similar Products]
 OT elisa kit Manual   
 Immunogen Sequence Length    126
 3D Structure    ModBase 3D Structure for E2GI53
 Species Reactivity    Rat
 Sample Type    serum, plasma, tissue homogenates
 Detection Range    15 pg/ml-600 pg/ml
 Detection Wavelength    450 nm
 Sensitivity    15 pg/ml
 Sample Volume    50-100ul
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for OT elisa kit    Small volumes of OT elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.

Product Description specifically for OT elisa kit

   Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for OT and Horseradish Peroxidase (HRP) conjugated OT. The competitive inhibition reaction is launched between with HRP labeled OT and unlabeled OT with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of OT in the sample. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for OT. It may not necessarily be applicable to this product.
 NCBI GI #    300675592 [Other Products]
 NCBI Accession #    ADK26458.1 [Other Products]
 UniProt Primary Accession #    E2GI53 [Other Products]
 Molecular Weight    13,427 Da
 NCBI Official Full Name    oxytocin
 UniProt Protein Name    Oxytocin
 UniProt Synonym Protein Names   


 UniProt Gene Name    Oxt [Similar Products]
 UniProt Entry Name    E2GI53_MESAU
 UniProt References for OT    1. "Oxytocin (Oxt) in the golden hamster."Meerts S.H., Venier J.E., Sisk C.L. Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases. Cited for: NUCLEOTIDE SEQUENCE. <p>Provides general information on the entry.</p><p><a href='../manual/entry_information_section' target='_top'>More...</a></p>Entry informationi<p>Provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.</p><p><a href='../manual/entry_name' target='_top'>More...</a></p>Entry nameiE2GI53_MESAU<p>Provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.</p><p><a href='../manual/accession_numbers' target='_top'>More...</a></p>AccessioniE2GI53Primary (citable) accession number: E2GI53<p>Shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the sequence are also displayed.</p><p><a href='../manual/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: November 30, 2010Last sequence update: November 30, 2010Last modified: July 9, 2014This is version 13 of the entry and version 1 of the sequence. [Complete history]<p>Indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).</p><p><a href='../manual/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)<p>Provides links to the UniProt Reference Clusters (<a href="/help/uniref">UniRef</a>). UniRef consists of clusters for UniProtKB sequences (including isoforms) and selected UniParc sequences, in order to obtain complete coverage of the sequence space at resolutions of 100%, 90% and 50% identity.</p><p><a href='../manual/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi50% IdentityEntryCluster member(s)OrganismsLengthCluster IDCluster nameSizeE2GI53P01175F1SWA3F1SWA2L8IM61UPI0002C46708UPI00042AA171UPI000016C4DCUPI00045DB6EFQ257C8+55Bos taurus (Bovine)Bos mutusTrichechus manatus latirostris (Florida manatee)Peromyscus maniculatus bairdii (prairie deer mouse)Ovis aries (Sheep)Orycteropus afer aferBubalus bubalis (Domestic water buffalo)Mustela putorius furo (European domestic ferret) (Mustela furo)Sus scrofa (Pig)+37125UniRef50_P01175Cluster: Oxytocin-neurophysin 165
 Searchable Name for OT    anti-OT; Buy OT elisa kit ($540), Rat oxytocin ELISA Kit (MBS700101) product datasheet at MyBioSource, ELISA Kits; E2GI53_MESAU; OT elisa kit; anti-OT
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
Products similar to Rat oxytocin, OT ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS7243274  Rat Oxytocin-neurophysin 1 ELISA Kit  MBS2601422  Rat Oxytocin-neurophysin 1 (OXT) ELISA Kit
 MBS269928  Human Oxytocin-neurophysin 1 (OXT) ELISA Kit  MBS703338  Human oxytocin, OT ELISA Kit
 MBS700794  Pig oxytocin, OT ELISA Kit
Products associated with OT elisa kitPathways associated with OT elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with OT and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with OT elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with OT and Bone
 Connective Tissue Antibodies  >4 publications with OT and Connective Tissue
 Heart Antibodies  >3 publications with OT and Heart
 Muscle Antibodies  >3 publications with OT and Muscle
 Brain Antibodies  >3 publications with OT and Brain
 Vascular Antibodies  >2 publications with OT and Vascular
 Embryonic Tissue Antibodies  >2 publications with OT and Embryonic Tissue
 Ear Antibodies  >1 publications with OT and Ear
 Kidney Antibodies  >1 publications with OT and Kidney
 Umbilical Cord Antibodies  >1 publications with OT and Umbilical Cord
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