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FT4 elisa kit :: Mouse Free Thyroxine, FT4 ELISA Kit

Catalog #    MBS700444 (PROMOTIONAL PRICING: 20% OFF list price for 96 Strip Wells Kit only.
Discount will be applied after order has been placed)
FT4 elisa kit
Unit / Price
96 Strip Wells  /  $515 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

Free Thyroxine, FT4, ELISA Kit

 Also Known As   

Mouse Free Thyroxine, FT4 ELISA Kit

 Product Synonym Names    Mouse Free Thyroxine; FT4 ELISA Kit; Mouse Free Thyroxine; FT4
 Product Gene Name   

FT4 elisa kit

[Similar Products]
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 FT4 elisa kit Manual   
 Immunogen Sequence Length    73
 3D Structure    ModBase 3D Structure for E3TSA0
 Species Reactivity    Mouse
 Sample Type    serum, plasma, tissue homogenates
 Detection Range    4 pmol/l-64 pmol/l
 Detection Wavelength    450 nm
 Sensitivity    1.343 pmol/L
 Sample Volume    50-100ul
 Protein Biological Process 1    Thyroid function
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for FT4 elisa kit    Small volumes of FT4 elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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Product Description specifically for FT4 elisa kit

   Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of FT4 in the sample. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for FT4. It may not necessarily be applicable to this product.
 NCBI GI #    309259079 [Other Products]
 NCBI Accession #    ADO61927.1 [Other Products]
 UniProt Primary Accession #    E3TSA0 [Other Products]
 Molecular Weight    8,491 Da
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 NCBI Official Full Name    flowering locus T4, partial
 UniProt Protein Name    Flowering locus T4
 UniProt Synonym Protein Names   

Flowering locus T4

 UniProt Gene Name    FT4 [Similar Products]
 UniProt Entry Name    E3TSA0_HELAN
 UniProt References for FT4    1. "Contributions of flowering time genes to sunflower domestication and improvement."Blackman B.K., Rasmussen D.A., Strasburg J.L., Raduski A.R., Burke J.M., Knapp S.J., Michaels S.D., Rieseberg L.H.Genetics 187:271-287(2011) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE. Strain: Ann1064ImportedAutomatic assertion inferred from database entries:EMBL:ADO61896.1, Ann113ImportedAutomatic assertion inferred from database entries:EMBL:ADO61917.1, Ann1200ImportedAutomatic assertion inferred from database entries:EMBL:ADO61924.1, Ann1238ImportedAutomatic assertion inferred from database entries:EMBL:ADO61882.1, Ann172ImportedAutomatic assertion inferred from database entries:EMBL:ADO61914.1, Ann1749ImportedAutomatic assertion inferred from database entries:EMBL:ADO61926.1, Ann1811ImportedAutomatic assertion inferred from database entries:EMBL:ADO61894.1, Ann185ImportedAutomatic assertion inferred from database entries:EMBL:ADO61912.1, Ann20ImportedAutomatic assertion inferred from database entries:EMBL:ADO61918.1, Ann203ImportedAutomatic assertion inferred from database entries:EMBL:ADO61884.1, Ann207ImportedAutomatic assertion inferred from database entries:EMBL:ADO61910.1, Ann2171ImportedAutomatic assertion inferred from database entries:EMBL:ADO61908.1, Ann2179ImportedAutomatic assertion inferred from database entries:EMBL:ADO61906.1, Ann2194ImportedAutomatic assertion inferred from database entries:EMBL:ADO61904.1, Ann2198ImportedAutomatic assertion inferred from database entries:EMBL:ADO61900.1, Ann2213ImportedAutomatic assertion inferred from database entries:EMBL:ADO61898.1, Ann2309ImportedAutomatic assertion inferred from database entries:EMBL:ADO61888.1, Ann2321ImportedAutomatic assertion inferred from database entries:EMBL:ADO61886.1, Ann2327ImportedAutomatic assertion inferred from database entries:EMBL:ADO61920.1, Ann423ImportedAutomatic assertion inferred from database entries:EMBL:ADO61890.1, Ann570ImportedAutomatic assertion inferred from database entries:EMBL:ADO61922.1, Ann881ImportedAutomatic assertion inferred from database entries:EMBL:ADO61902.1 and Ann886ImportedAutomatic assertion inferred from database entries:EMBL:ADO61892.1. <p>Provides general information on the entry.</p><p><a href='../manual/entry_information_section' target='_top'>More...</a></p>Entry informationi<p>Provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.</p><p><a href='../manual/entry_name' target='_top'>More...</a></p>Entry nameiE3TSA0_HELAN<p>Provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.</p><p><a href='../manual/accession_numbers' target='_top'>More...</a></p>AccessioniE3TSA0Primary (citable) accession number: E3TSA0<p>Shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the sequence are also displayed.</p><p><a href='../manual/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: January 11, 2011Last sequence update: January 11, 2011Last modified: February 19, 2014This is version 11 of the entry and version 1 of the sequence. [Complete history]<p>Indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).</p><p><a href='../manual/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)<p>Provides links to the UniProt Reference Clusters (<a href="/help/uniref">UniRef</a>). UniRef consists of clusters for UniProtKB sequences (including isoforms) and selected UniParc sequences, in order to obtain complete coverage of the sequence space at resolutions of 100%, 90% and 50% identity.</p><p><a href='../manual/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi100% Identity90% Identity50% IdentityEntryCluster member(s)OrganismsLengthCluster IDCluster nameSizeE3TSA0I3PLQ4E3TS88E3TS90D5I4V6D5I4R1Xanthium strumariumHelianthus argophyllusHelianthus annuus (Common sunflower)176UniRef100_I3PLQ4Cluster: Flowering locus T 36Full viewEntryCluster member(s)OrganismsLengthCluster IDCluster nameSizeE3TSA0I3PLQ4E3TS88E3TS90D5I4V6D5I4R1F1T158K0A5S9I3PLQ2+10Xanthium strumariumHelianthus argophyllusHelianthus annuus (Common sunflower)Lactuca sativa (Garden lettuce)Gazania linearisChrysanthemum lavandulifolium (Daisy) (Dendranthema lavandulifolium)Chrysanthemum morifolium (Florist's daisy) (Dendranthema grandiflorum)Chrysanthemum seticuspe f. boreale176UniRef90_I3PLQ4Cluster: Flowering locus T 320Full viewEntryCluster member(s)OrganismsLengthCluster IDCluster nameSizeE3TSA0Q9SXZ2Q84XX3N0A632R0IA71D6RRE5D7KRW2V7CWN1I3RUU9I3RUR8+385Arabidopsis thaliana (Mouse-ear cress)Brassica rapa subsp. pekinensis (Chinese cabbage) (Brassica pekinensis)Arabidopsis halleri subsp. gemmifera (Arabis gemmifera)Capsella rubellaArabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress)Phaseolus vulgaris (Kidney bean) (French bean)Oryza rufipogon (Brownbeard rice) (Asian wild rice)Oryza sativa subsp. japonica (Rice)Oryza glaberrima (African rice)+123175UniRef50_Q9SXZ2Cluster: Protein FLOWERING LOCUS T395
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 Searchable Name for FT4    anti-FT4; Buy FT4 elisa kit ($515), Mouse Free Thyroxine, FT4 ELISA Kit (MBS700444) product datasheet at MyBioSource, ELISA Kits; E3TSA0_HELAN; FT4 elisa kit; anti-FT4
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with FT4 elisa kitPathways associated with FT4 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with FT4 and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
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 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with FT4 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with FT4 and Bone
 Connective Tissue Antibodies  >4 publications with FT4 and Connective Tissue
 Heart Antibodies  >3 publications with FT4 and Heart
 Muscle Antibodies  >3 publications with FT4 and Muscle
 Brain Antibodies  >3 publications with FT4 and Brain
 Vascular Antibodies  >2 publications with FT4 and Vascular
 Embryonic Tissue Antibodies  >2 publications with FT4 and Embryonic Tissue
 Ear Antibodies  >1 publications with FT4 and Ear
 Kidney Antibodies  >1 publications with FT4 and Kidney
 Umbilical Cord Antibodies  >1 publications with FT4 and Umbilical Cord
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