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Human L-Carnitine ELISA Kit

Catalog #    MBS701318 L-Carnitine elisa kit
Unit / Price
48 Strip Wells  /  $600 +1 FREE 8GB USB
96 Strip Wells  /  $865 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

L-Carnitine, ELISA Kit

 Also Known As   

Human L-Carnitine ELISA Kit

 Product Synonym Names    Human L-Carnitine ELISA Kit; L-Carnitine
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 L-Carnitine elisa kit Manual   
 Immunogen Sequence Length    505
 Species Reactivity    Human
 Sample Type    serum, plasma, urine, tissue homogenates
 Detection Range    0.312 ng/ml-20 ng/ml
 Detection Wavelength    450 nm
 Sensitivity    0.078 ng/ml
 Sample Volume    50-100ul
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for L-Carnitine elisa kit    Small volumes of L-Carnitine elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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Product Description specifically for L-Carnitine elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to L-Carnitine. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for L-Carnitine and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain L-Carnitine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of L-Carnitine in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI/Uniprot data below describe general gene information for L-Carnitine. It may not necessarily be applicable to this product.
 NCBI GI #    660588941 [Other Products]
 NCBI Accession #    AIE04004.1 [Other Products]
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 NCBI Official Full Name    L-carnitine
 Searchable Name for L-Carnitine    anti-L-Carnitine; Buy L-Carnitine elisa kit ($600), Human L-Carnitine ELISA Kit (MBS701318) product datasheet at MyBioSource, ELISA Kits; L-Carnitine elisa kit; anti-L-Carnitine
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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