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HEXA elisa kit :: hexosaminidase A (alpha polypeptide) [ELISA Kit]
Catalog #    MBS701489
Unit / Price
48 Strip Wells  /  $630.00 + 1 FREE 8GB USB Flash Drive
96 Strip Wells  /  $910.00 + 1 FREE 8GB USB Flash Drive
    
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    hexosaminidase A (alpha polypeptide) , ELISA Kit
 Also Known As    Mouse beta-hexosaminidase A, beta-Hex A ELISA Kit
 Product Synonym Names    Mouse beta-hexosaminidase A; beta-Hex A ELISA Kit; MGC99608; TSD; N-acetyl-beta-glucosaminidase; beta-N-acetylhexosaminidase; hexosaminidase A; hexosaminidase A (alpha polypeptide)
 Product Gene Name    HEXA elisa kit [Similar Products]
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 HEXA elisa kit Manual   
 Immunogen Sequence Length    528
 3D Structure    ModBase 3D Structure for P29416
 Species Reactivity    Mouse
 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse HEXA. No significant cross-reactivity or interference between Mouse HEXA and analogues was observed.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Sample Type    serum, plasma, tissue homogenates
 Detection Range    3.12 uU/ml-200 uU/ml
 Detection Wavelength    450 nm
 Sensitivity    0.78 uU/ml
 Sample Volume    50-100ul
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for HEXA elisa kit    Small volumes of HEXA elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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 Product Description specifically for HEXA elisa kit    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-Hex A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-Hex A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-Hex A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-Hex A bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for HEXA. It may not necessarily be applicable to this product.
 NCBI GI #    67514549 [Other Products]
 NCBI GeneID    15211 [Other Products]
 NCBI Accession #    NP_034551.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_010421.4 [Other Products]
 UniProt Primary Accession #    P29416 [Other Products]
 UniProt Secondary Accession #    Q64246; Q91XG3 [Other Products]
 UniProt Related Accession #    P29416 [Other Products]
 Molecular Weight    60,613 Da
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 NCBI Official Full Name    beta-hexosaminidase subunit alpha
 NCBI Official Synonym Full Names    hexosaminidase A
 NCBI Official Symbol    Hexa [Similar Products]
 NCBI Official Synonym Symbols    Hex-1 [Similar Products]
 NCBI Protein Information    beta-hexosaminidase subunit alpha; hexosaminidase subunit A; beta-N-acetylhexosaminidase subunit alpha; N-acetyl-beta-glucosaminidase subunit alpha
 UniProt Protein Name    Beta-hexosaminidase subunit alpha
 UniProt Synonym Protein Names    Beta-N-acetylhexosaminidase subunit alpha; Hexosaminidase subunit A; N-acetyl-beta-glucosaminidase subunit alpha
 UniProt Gene Name    Hexa [Similar Products]
 UniProt Synonym Gene Names    Hexosaminidase subunit A [Similar Products]
 UniProt Entry Name    HEXA_MOUSE
 UniProt Comments for HEXA    Function: Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.

Catalytic activity: Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
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 UniProt References for HEXA    1. "Cloning and sequence analysis of a cDNA encoding the alpha-subunit of mouse beta-N-acetylhexosaminidase and comparison with the human enzyme."Beccari T., Hoade J., Orlacchio A., Stirling J.L.Biochem. J. 285:593-596(1992) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
2. "Organization of the gene for the alpha-subunit of mouse beta-N-acetylhexosaminidase (HEXa)."Bianconi S., Beccari T., Stirling J.L., Sheardown S., Orlacchio A.Biochem. Mol. Biol. Int. 34:579-586(1994) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
3. "Structure and expression of the mouse beta-hexosaminidase genes, Hexa and Hexb."Yamanaka S., Johnson O.N., Norflus F., Boles D.J., Proia R.L.Genomics 21:588-596(1994) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6 X CBA. Tissue: Liver.
4. "Structural organization, sequence, and expression of the mouse HEXA gene encoding the alpha subunit of hexosaminidase A."Wakamatsu N., Benoit G., Lamhonwah A.-M., Zhang Z.-X., Trasler J.M., Triggs-Raine B.L., Gravel R.A.Genomics 24:110-119(1994) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: 129/Sv. Tissue: Liver.
5. "The transcriptional landscape of the mammalian genome."Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: BALB/c and C57BL/6J. Tissue: Tongue.
 Research Articles on HEXA    1. Neu4 is not the only sialidase contributing to the metabolic bypass in Hexa(-/-) mice.
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 Searchable Name for HEXA    anti-HEXA; Buy HEXA elisa kit :: hexosaminidase A (alpha polypeptide) , ELISA Kit (MBS701489) datasheet at MyBioSource; anti-HEXA; Hex-1; Hexosaminidase subunit A; anti-hexosaminidase A (alpha polypeptide) ; Mouse beta-hexosaminidase A, beta-Hex A ELISA Kit; Mouse beta-hexosaminidase A; beta-Hex A ELISA Kit; MGC99608; TSD; N-acetyl-beta-glucosaminidase; beta-N-acetylhexosaminidase; hexosaminidase A; hexosaminidase A (alpha polypeptide) ; beta-hexosaminidase subunit alpha; hexosaminidase A; Beta-hexosaminidase subunit alpha; Beta-N-acetylhexosaminidase subunit alpha; Hexosaminidase subunit A; N-acetyl-beta-glucosaminidase subunit alpha anti-Mouse. (NCBI/UniProt 67514549 NP_034551.2 P29416 NM_010421.4 P29416 Q64246; Q91XG3 60,613 Da); 15211; HEXA_MOUSE; HEXA elisa kit; anti-HEXA
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with HEXA elisa kitPathways associated with HEXA elisa kit
 Reference Product  PubMed Publications
 Hexb elisa kit  >79 publications with HEXA and Hexb
 Gm2a elisa kit  >9 publications with HEXA and Gm2a
 Naga elisa kit  >4 publications with HEXA and Naga
 Gusb elisa kit  >3 publications with HEXA and Gusb
 Glb1 elisa kit  >2 publications with HEXA and Glb1
 Neu4 elisa kit  >1 publications with HEXA and Neu4
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Diseases associated with HEXA elisa kitOrgans/Tissues associated with HEXA elisa kit
 Disease Name  Pubmed Publications
 Tay-Sachs Disease Antibodies  >131 publications with HEXA and Tay-Sachs Disease
 Hepatitis Antibodies  >60 publications with HEXA and Hepatitis
 Liver Diseases Antibodies  >38 publications with HEXA and Liver Diseases
 Inflammation Antibodies  >36 publications with HEXA and Inflammation
 Carcinoma Antibodies  >33 publications with HEXA and Carcinoma
 Death Antibodies  >29 publications with HEXA and Death
 Necrosis Antibodies  >27 publications with HEXA and Necrosis
 Poisoning Antibodies  >22 publications with HEXA and Poisoning
 Cardiovascular Diseases Antibodies  >20 publications with HEXA and Cardiovascular Diseases
 Kidney Diseases Antibodies  >19 publications with HEXA and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >281 publications with HEXA and Blood
 Liver Antibodies  >140 publications with HEXA and Liver
 Brain Antibodies  >105 publications with HEXA and Brain
 Kidney Antibodies  >65 publications with HEXA and Kidney
 Lung Antibodies  >40 publications with HEXA and Lung
 Intestine Antibodies  >39 publications with HEXA and Intestine
 Testis Antibodies  >18 publications with HEXA and Testis
 Stomach Antibodies  >15 publications with HEXA and Stomach
 Placenta Antibodies  >12 publications with HEXA and Placenta
 Eye Antibodies  >12 publications with HEXA and Eye
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