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MUC5AC elisa kit :: mucin-5AC (MUC5AC) [ELISA Kit]
Catalog #    MBS701926
Unit / Price
48 Strip Wells  /  $645.00 + 1 FREE 8GB USB Flash Drive
96 Strip Wells  /  $880.00 + 1 FREE 8GB USB Flash Drive
    
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    mucin-5AC (MUC5AC), ELISA Kit
 Also Known As    Human mucin-5AC (MUC5AC) ELISA Kit
 Product Synonym Names    mucin-5AC; MUC5; gastric mucin|mucin 5; subtypes A and C; tracheobronchial/gastric|mucin 5AC|tracheobronchial mucin
 Product Gene Name    MUC5AC elisa kit [Similar Products]
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 MUC5AC elisa kit Manual   
 Immunogen Sequence Length    67
 OMIM    158373
 3D Structure    ModBase 3D Structure for P98088
 Species Reactivity    Human
 Specificity    This assay has high sensitivity and excellent specificity for detection of human MUC5AC. No significant cross-reactivity or interference between human MUC5AC and analogues was observed.
 Samples    For the quantitative determination of human mucin-5AC (MUC5AC) concentrations in serum, plasma, cell culture supernates, urine.
 Detection Range    15.6 ng/ml-1000 ng/ml.
 Sensitivity    The minimum detectable dose of human MUC5AC is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for MUC5AC elisa kit    Small volumes of MUC5AC elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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 Product Description specifically for MUC5AC elisa kit    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC5AC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC5AC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC5AC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC5AC bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for MUC5AC. It may not necessarily be applicable to this product.
 NCBI GI #    15026993 [Other Products]
 NCBI Accession #    CAC44892.1 [Other Products]
 UniProt Primary Accession #    P98088 [Other Products]
 UniProt Secondary Accession #    O60460; O76065; Q13792; Q14425; Q658Q1; Q7M4S5; Q8N4M9; Q8WWQ3; Q8WWQ4; Q8WWQ5 [Other Products]
 Molecular Weight    526,608 Da
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 NCBI Official Full Name    MUC5AC protein
 UniProt Protein Name    Mucin-5AC
 UniProt Synonym Protein Names    Gastric mucin; Lewis B blood group antigen; LeB; Major airway glycoprotein; Mucin-5 subtype AC, tracheobronchial; Tracheobronchial mucin
 UniProt Gene Name    MUC5AC [Similar Products]
 UniProt Synonym Gene Names    MUC5; MUC-5AC; LeB; TBM [Similar Products]
 UniProt Entry Name    MUC5A_HUMAN
 UniProt Comments for MUC5AC    Function: Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.

Subunit structure: Multimeric. Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD). Ref.12

Subcellular location: Secreted Ref.13.

Tissue specificity: Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. Ref.6 Ref.12

Domain: The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding.

Post-translational modification: C-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis. Ref.11 Ref.13Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide.

Sequence similarities: Contains 1 CTCK (C-terminal cystine knot-like) domain.Contains 2 VWFC domains.Contains 4 VWFD domains.

Sequence caution: The sequence AAA18431.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAC15950.1 differs from that shown. Reason: Frameshift at positions 24, 44, 671 and 683. The sequence CAA88307.1 differs from that shown. Reason: Frameshift at position 5024. The sequence CAH56330.1 differs from that shown. Reason: Frameshift at position 4616.
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 UniProt References for MUC5AC    • Human mucin gene MUC5AC: organization of its 5'-region and central repetitive region. Escande F., Aubert J.-P., Porchet N., Buisine M.P.Biochem. J. 358:763-772(2001) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-2448, NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2449-3797, NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3798-4169.
• Cloning of the amino-terminal and 5'-flanking region of the human MUC5AC mucin gene and transcriptional up-regulation by bacterial exoproducts. Li D., Gallup M., Fan N., Szymkowski D.E., Basbaum C.B.J. Biol. Chem. 273:6812-6820(1998) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-1104. Tissue: Trachea.
• Cloning and analysis of human gastric mucin cDNA reveals two types of conserved cysteine-rich domains. Klomp L.W., Van Rens L., Strous G.J.Biochem. J. 308:831-838(1995) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1005-1854.
• Proteolytic fragmentation and peptide mapping of human carboxyamidomethylated tracheobronchial mucin. Rose M.C., Kaufman B., Martin B.M.J. Biol. Chem. 264:8193-8199(1989) [PubMed] [Europe PMC] [Abstract]Cited for: PROTEIN SEQUENCE OF 1752-1773; 1796-1805; 2125-2146; 2169-2178; 2655-2676; 2697-2708; 2953-2974; 2997-3006; 3386-3407; 3428-3439 AND 4012-4033. Tissue: Tracheobronchial mucosa.
• Genomic organization of the 3'-region of the human MUC5AC mucin gene: additional evidence for a common ancestral gene for the 11p15.5 mucin gene family. Buisine M.P., Desseyn J.-L., Porchet N., Degand P., Laine A., Aubert J.-P.Biochem. J. 332:729-738(1998) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 3950-5030, VARIANT PRO-4897. Tissue: Placenta and Trachea.
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 Searchable Name for MUC5AC    anti-MUC5AC; Buy MUC5AC elisa kit :: mucin-5AC (MUC5AC), ELISA Kit (MBS701926) datasheet at MyBioSource; anti-MUC5AC; MUC5; MUC-5AC; LeB; TBM; anti-mucin-5AC (MUC5AC); Human mucin-5AC (MUC5AC) ELISA Kit; mucin-5AC; MUC5; gastric mucin|mucin 5; subtypes A and C; tracheobronchial/gastric|mucin 5AC|tracheobronchial mucin; MUC5AC protein; Mucin-5AC; Gastric mucin; Lewis B blood group antigen; LeB; Major airway glycoprotein; Mucin-5 subtype AC, tracheobronchial; Tracheobronchial mucin anti-Human. (NCBI/UniProt 15026993 CAC44892.1 P98088 O60460; O76065; Q13792; Q14425; Q658Q1; Q7M4S5; Q8N4M9; Q8WWQ3; Q8WWQ4; Q8WWQ5 526,608 Da); MUC5A_HUMAN; MUC5AC elisa kit; anti-MUC5AC
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with MUC5AC elisa kitPathways associated with MUC5AC elisa kit
 Product Name  Ref Prod  PubMed Publications
 MUC5AC elisa kit  FLNA elisa kit  >12 publications with MUC5AC and FLNA
 Product Name  Products by Pathway  Pathway Diagram
 MUC5AC elisa kit  Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MUC5AC elisa kit  Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MUC5AC elisa kit  MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MUC5AC elisa kit  MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MUC5AC elisa kit  Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 MUC5AC elisa kit  Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with MUC5AC elisa kit
 Organ/Tissue Name  Ref Prod  Pubmed Publications
 Bone Antibodies  MUC5AC antibody  >13 publications with MUC5AC and Bone
 Connective Tissue Antibodies  MUC5AC antibody  >4 publications with MUC5AC and Connective Tissue
 Heart Antibodies  MUC5AC antibody  >3 publications with MUC5AC and Heart
 Muscle Antibodies  MUC5AC antibody  >3 publications with MUC5AC and Muscle
 Brain Antibodies  MUC5AC antibody  >3 publications with MUC5AC and Brain
 Vascular Antibodies  MUC5AC antibody  >2 publications with MUC5AC and Vascular
 Embryonic Tissue Antibodies  MUC5AC antibody  >2 publications with MUC5AC and Embryonic Tissue
 Ear Antibodies  MUC5AC antibody  >1 publications with MUC5AC and Ear
 Kidney Antibodies  MUC5AC antibody  >1 publications with MUC5AC and Kidney
 Umbilical Cord Antibodies  MUC5AC antibody  >1 publications with MUC5AC and Umbilical Cord
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