MyBioSource
  MyBioSource   MBS702132   LOG IN   MY ACCOUNT   CART CONTENTS   CHECKOUT 
MyBioSource Menu
 ELISA Kit
 Monoclonal Antibody
 Polyclonal Antibody
 Secondary Antibody
 Antigen
 Biochemical
 cDNA Clone
 Peptide
 Rec./Purified Protein

 Antibody Matched Pairs
 Products by Disease
 Products by Pathway

 Advanced Search
 Submit Technical Q&A
 International Distributors
 Contact Us


Our Best Sellers more
 • Cytomegalovirus, Pp65 Tegument Protein, Antibody
 • IgG , Antibody
 • Mitochondrial brown fat uncoupling protein 1 (UCP1), ELISA Kit
 • Pituitary Adenylate Cyclase Activating Polypeptide Receptor 1, ELISA Kit
 • BMP7 (monomer), Recombinant Protein
 • Protein FAM3C (FAM3C), ELISA Kit
 • Vitamin D-binding protein, DBP, ELISA Kit
 • Mullerian Inhibiting Substance/Anti Mullerian Hormone, ELISA Kit
 • solute carrier family 27 (fatty acid transporter), member 3, Antibody
 • Calcium/calmodulin-dependent protein kinase type II subunit alpha (CAMK2A), ELISA Kit
  more ...
Email to a Friend
 
Tell someone you know about this product.
GAD2 elisa kit :: glutamic acid decarboxylase 65, GAD65 [ELISA Kit]
Catalog #    MBS702132
Unit / Price
48 Strip Wells  /  $585.00 + 1 FREE 8GB USB Flash Drive
96 Strip Wells  /  $775.00 + 1 FREE 8GB USB Flash Drive
    
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    glutamic acid decarboxylase 65, GAD65, ELISA Kit
 Also Known As    Human glutamic acid decarboxylase 65, GAD65 ELISA Kit
 Product Synonym Names    glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); RP11-420F12.2; GAD65; MGC161605; MGC161607; Glutamate decarboxylase-2 (pancreas)|glutamate decarboxylase 2
 Product Gene Name    GAD2 elisa kit [Similar Products]
TOP
 GAD2 elisa kit Manual   
 Immunogen Sequence Length    585
 Chromosome Location    Chromosome: 10; NC_000010.10 (26505236..26593491). Location: 10p11.23
 OMIM    138275
 3D Structure    ModBase 3D Structure for Q5VZ30
 Species Reactivity    Human
 Specificity    This assay has high sensitivity and excellent specificity for detection of human GAD65. No significant cross-reactivity or interference between human GAD65 and analogues was observed.
 Samples    For the quantitative determination of human glutamic acid decarboxylase 65 (GAD65) concentrations in serum, plasma, tissue homogenates.
 Detection Range    0.156 ng/ml-10 ng/ml.
 Sensitivity    The minimum detectable dose of human GAD65 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for GAD2 elisa kit    Small volumes of GAD2 elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
TOP
 Product Description specifically for GAD2 elisa kit    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GAD65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAD65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAD65 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAD65 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for GAD2. It may not necessarily be applicable to this product.
 NCBI GI #    55662653 [Other Products]
 NCBI GeneID    2572 [Other Products]
 NCBI Accession #    NP_000809.1 [Other Products]
 UniProt Primary Accession #    Q5VZ30 [Other Products]
 UniProt Secondary Accession #    Q9UD87 [Other Products]
 UniProt Related Accession #    Q05329 [Other Products]
 Molecular Weight    65,411 Da
TOP
 NCBI Official Full Name    glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
 NCBI Official Synonym Full Names    glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
 NCBI Official Symbol    GAD2 [Similar Products]
 NCBI Official Synonym Symbols    GAD65 [Similar Products]
 NCBI Protein Information    glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform
 UniProt Protein Name    Glutamate decarboxylase 2
 UniProt Synonym Protein Names    65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
 UniProt Gene Name    GAD2 [Similar Products]
 UniProt Synonym Gene Names    GAD65; GAD-65 [Similar Products]
 UniProt Entry Name    DCE2_HUMAN
 NCBI Summary for GAD2    This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
 UniProt Comments for GAD2    Function: Catalyzes the production of GABA.

Catalytic activity: L-glutamate = 4-aminobutanoate + CO2.

Cofactor: Pyridoxal phosphate. Ref.10

Subunit structure: Homodimer. Ref.10

Subcellular location: Cytoplasm › cytosol. Cytoplasmic vesicle. Cell junction › synapse › presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. Ref.8

Post-translational modification: Phosphorylated; which does not affect kinetic parameters or subcellular location. Ref.7Palmitoylated; which is required for presynaptic clustering. Ref.8

Sequence similarities: Belongs to the group II decarboxylase family.

Biophysicochemical propertiesKinetic parameters:KM=2.15 mM for glutamate Ref.7

Sequence caution: The sequence CAA49554.1 differs from that shown. Reason: Erroneous initiation.
TOP
 NCBI References for GAD2    1. GADA titer-related risk for organ-specific autoimmunity in LADA subjects subdivided according to gender (NIRAD study 6).
2. Reduced glutamate decarboxylase 65 protein within primary auditory cortex inhibitory boutons in schizophrenia.
3. Identification of novel DNA methylation markers in colorectal cancer using MIRA-based microarrays.
4. HLA-DQB1 genotypes and islet cell autoantibodies against GAD65 and IA-2 in relation to development of diabetes post partum in women with gestational diabetes mellitus.
5. Palmitoylation and trafficking of GAD65 are impaired in a cellular model of Huntington's disease.
 UniProt References for GAD2    • Cloning and primary structure of a human islet isoform of glutamic acid decarboxylase from chromosome 10. Karlsen A.E., Hagopian W.A., Grubin C.E., Dube S., Disteche C.M., Adler D.A., Barmeier H., Mathewes S., Grant F.J., Foster D., Lernmark A.Proc. Natl. Acad. Sci. U.S.A. 88:8337-8341(1991) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreatic islet.
• Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene. Bu D.-F., Erlander M.G., Hitz B.C., Tillakaratne N.J., Kaufman D.L., Wagner-Mcpherson C.B., Evans G.A., Tobin A.J.Proc. Natl. Acad. Sci. U.S.A. 89:2115-2119(1992) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA].
• The exon-intron organization of the genes (GAD1 and GAD2) encoding two human glutamate decarboxylases (GAD67 and GAD65) suggests that they derive from a common ancestral GAD. Bu D.-F., Tobin A.J.Genomics 21:222-228(1994) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. [4]NIEHS SNPs programSubmitted (JUL-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-12; ASN-124; ARG-286 AND GLN-375.
• Characterization of a linear epitope within the human pancreatic 64-kDa glutamic acid decarboxylase and its autoimmune recognition by sera from insulin-dependent diabetes mellitus patients. Mauch L., Abney C.C., Berg H., Scherbaum W.A., Liedvogel B., Northemann W.Eur. J. Biochem. 212:597-603(1993) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-585. Tissue: Pancreas.
 Research Articles on GAD2    1. Decreased expression of GAD65 protein within inhibitory boutons could contribute to auditory impairments in schizophrenia.
TOP
 Searchable Name for GAD2    anti-GAD2; Buy GAD2 elisa kit :: glutamic acid decarboxylase 65, GAD65, ELISA Kit (MBS702132) datasheet at MyBioSource; anti-GAD2; GAD65; GAD65; GAD-65; anti-glutamic acid decarboxylase 65, GAD65; Human glutamic acid decarboxylase 65, GAD65 ELISA Kit; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); RP11-420F12.2; GAD65; MGC161605; MGC161607; Glutamate decarboxylase-2 (pancreas)|glutamate decarboxylase 2; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform anti-Human. (NCBI/UniProt 55662653 NP_000809.1 Q05329 Q5VZ30 Q9UD87 65,411 Da); Chromosome: 10; NC_000010.10 (26505236..26593491). Location: 10p11.23; 2572; DCE2_HUMAN; GAD2 elisa kit; anti-GAD2
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
TOP
Products similar to Human glutamic acid decarboxylase 65, GAD65 ELISA Kit...
 Catalog #  Product Name  Catalog #  Product Name
 MBS755705  Human Anti-Glutamic Acid Decarboxylase Antibodies 65 ELISA K... (ELISA Kit)  MBS756330  Rabbit Anti-Glutamic Acid Decarboxylase Antibodies 65 ELISA ... (ELISA Kit)
 MBS756345  Chicken Anti-Glutamic Acid Decarboxylase Antibodies 65 ELISA... (ELISA Kit)  MBS755968  Monkey Anti-Glutamic Acid Decarboxylase Antibodies 65 ELISA ... (ELISA Kit)
 MBS756123  Goat Anti-Glutamic Acid Decarboxylase Antibodies 65 ELISA Ki... (ELISA Kit)  MBS755750  Sheep Anti-Glutamic Acid Decarboxylase Antibodies 65 ELISA K... (ELISA Kit)
 MBS756127  Rat Anti-Glutamic Acid Decarboxylase Antibodies 65 ELISA Kit (ELISA Kit)  MBS755753  Porcine Anti-Glutamic Acid Decarboxylase Antibodies 65 ELISA... (ELISA Kit)
 MBS755925  Canine Anti-Glutamic Acid Decarboxylase Antibodies 65 ELISA ... (ELISA Kit)  MBS756012  Guinea Pig Anti-Glutamic Acid Decarboxylase Antibodies 65 EL... (ELISA Kit)
Products associated with GAD2 elisa kitPathways associated with GAD2 elisa kit
 Product Name  Ref Prod  PubMed Publications
 GAD2 elisa kit  GAD1 elisa kit  >36 publications with GAD2 and GAD1
 Product Name  Products by Pathway  Pathway Diagram
 GAD2 elisa kit  Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 GAD2 elisa kit  Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 GAD2 elisa kit  Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 GAD2 elisa kit  Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 GAD2 elisa kit  Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GAD2 elisa kit  Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GAD2 elisa kit  GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GAD2 elisa kit  GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GAD2 elisa kit  GABA Synthesis Pathway antibodies  GABA Synthesis Pathway Diagram
 GAD2 elisa kit  GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram
Diseases associated with GAD2 elisa kitOrgans/Tissues associated with GAD2 elisa kit
 Disease Name  Ref Prod  Pubmed Publications
 Mental Disorders Antibodies  anti-GAD2  >14 publications with GAD2 and Mental Disorders
 Nervous System Diseases Antibodies  anti-GAD2  >11 publications with GAD2 and Nervous System Diseases
 Brain Diseases Antibodies  anti-GAD2  >9 publications with GAD2 and Brain Diseases
 Substance-Related Disorders Antibodies  anti-GAD2  >6 publications with GAD2 and Substance-Related Disorders
 Schizophrenia Antibodies  anti-GAD2  >6 publications with GAD2 and Schizophrenia
 Epilepsy Antibodies  anti-GAD2  >5 publications with GAD2 and Epilepsy
 Seizures Antibodies  anti-GAD2  >5 publications with GAD2 and Seizures
 Anxiety Disorders Antibodies  anti-GAD2  >3 publications with GAD2 and Anxiety Disorders
 Mood Disorders Antibodies  anti-GAD2  >3 publications with GAD2 and Mood Disorders
 Drug Toxicity Antibodies  anti-GAD2  >2 publications with GAD2 and Drug Toxicity
 Organ/Tissue Name  Ref Prod  Pubmed Publications
 Brain Antibodies  GAD2 antibody  >22 publications with GAD2 and Brain
 Pancreas Antibodies  GAD2 antibody  >2 publications with GAD2 and Pancreas
TOP