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GAD2 elisa kit :: Human glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) ELISA Kit

Catalog #    MBS702132 GAD2 elisa kit
Unit / Price
48 Strip Wells  /  $540 +1 FREE 8GB USB
96 Strip Wells  /  $775 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) (GAD2), ELISA Kit

 Also Known As   

Human glutamic acid decarboxylase 65, GAD65 ELISA Kit

 Product Synonym Names    Human glutamic acid decarboxylase 65; GAD65 ELISA Kit; RP11-420F12.2; GAD65; MGC161605; MGC161607; Glutamate decarboxylase-2 (pancreas) ; glutamate decarboxylase 2; glutamate decarboxylase 2 (pancreatic islets and brain; 65kDa)
 Product Gene Name   

GAD2 elisa kit

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 GAD2 elisa kit Manual   
 Immunogen Sequence Length    585
 OMIM    138275
 3D Structure    ModBase 3D Structure for Q05329
 Species Reactivity    Human
 Specificity    This assay has high sensitivity and excellent specificity for detection of human GAD65 . No significant cross-reactivity or interference between human GAD65 and analogues was observed.
 Samples    For the quantitative determination of human glutamic acid decarboxylase 65 (GAD65) concentrations in serum, plasma, tissue homogenates.
 Detection Range    0.156 ng/ml -10 ng/ml .
 Sensitivity    The minimum detectable dose of human GAD65 and is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for GAD2 elisa kit    Small volumes of GAD2 elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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Product Description specifically for GAD2 elisa kit

   Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GAD65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAD65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAD65 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAD65 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for GAD2. It may not necessarily be applicable to this product.
 NCBI GI #    4503875 [Other Products]
 NCBI GeneID    2572 [Other Products]
 NCBI Accession #    NP_000809.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000818.2 [Other Products]
 UniProt Primary Accession #    Q05329 [Other Products]
 UniProt Secondary Accession #    Q9UD87 [Other Products]
 UniProt Related Accession #    Q05329 [Other Products]
 Molecular Weight    65,411 Da
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 NCBI Official Full Name    glutamate decarboxylase 2
 NCBI Official Synonym Full Names    glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
 NCBI Official Symbol    GAD2 [Similar Products]
 NCBI Official Synonym Symbols   

GAD65

[Similar Products]
 NCBI Protein Information    glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform
 UniProt Protein Name    Glutamate decarboxylase 2
 UniProt Synonym Protein Names   

65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

 UniProt Gene Name    GAD2 [Similar Products]
 UniProt Synonym Gene Names    GAD65; GAD-65 [Similar Products]
 UniProt Entry Name    DCE2_HUMAN
 NCBI Summary for GAD2    This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
 UniProt Comments for GAD2    Function: Catalyzes the production of GABA.

Catalytic activity: L-glutamate = 4-aminobutanoate + CO2.

Cofactor: Pyridoxal phosphate.1 PublicationManual assertion based on experiment in:Ref.10
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 UniProt References for GAD2    1. "Cloning and primary structure of a human islet isoform of glutamic acid decarboxylase from chromosome 10."Karlsen A.E., Hagopian W.A., Grubin C.E., Dube S., Disteche C.M., Adler D.A., Barmeier H., Mathewes S., Grant F.J., Foster D., Lernmark A.Proc. Natl. Acad. Sci. U.S.A. 88:8337-8341(1991) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreatic islet.
2. "Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene."Bu D.-F., Erlander M.G., Hitz B.C., Tillakaratne N.J., Kaufman D.L., Wagner-Mcpherson C.B., Evans G.A., Tobin A.J.Proc. Natl. Acad. Sci. U.S.A. 89:2115-2119(1992) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
3. "The exon-intron organization of the genes (GAD1 and GAD2) encoding two human glutamate decarboxylases (GAD67 and GAD65) suggests that they derive from a common ancestral GAD."Bu D.-F., Tobin A.J.Genomics 21:222-228(1994) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA]. NIEHS SNPs program Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-12; ASN-124; ARG-286 AND GLN-375.
4. "Characterization of a linear epitope within the human pancreatic 64-kDa glutamic acid decarboxylase and its autoimmune recognition by sera from insulin-dependent diabetes mellitus patients."Mauch L., Abney C.C., Berg H., Scherbaum W.A., Liedvogel B., Northemann W.Eur. J. Biochem. 212:597-603(1993) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-585. Tissue: Pancreas.
5. "Differential expression of GAD65 and GAD67 in human, rat, and mouse pancreatic islets."Kim J., Richter W., Aanstoot H.-J., Shi Y., Fu Q., Rajotte R., Warnock G., Baekkeskov S.Diabetes 42:1799-1808(1993) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 245-585.
 Research Articles on GAD2    1. GAD65 expression in 313 gastric cancer tissues was significantly higher than in 60 adjacent non-tumour tissues. Moreover, the expression level of GAD65 significantly correlated with the depth of tumour invasion and TNM stage.
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 Searchable Name for GAD2    anti-GAD2; Buy GAD2 elisa kit ($540), Human glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) ELISA Kit (MBS702132) product datasheet at MyBioSource, ELISA Kits; 2572; DCE2_HUMAN; GAD2 elisa kit; anti-GAD2
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with GAD2 elisa kitPathways associated with GAD2 elisa kit
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 GAD1 elisa kit  >48 publications with GAD2 and GAD1
 GLUL elisa kit  >1 publications with GAD2 and GLUL
 INS elisa kit  >1 publications with GAD2 and INS
 SLC32A1 elisa kit  >1 publications with GAD2 and SLC32A1
 ABAT elisa kit  >1 publications with GAD2 and ABAT
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Diseases associated with GAD2 elisa kitOrgans/Tissues associated with GAD2 elisa kit
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >18 publications with GAD2 and Mental Disorders
 Nervous System Diseases Antibodies  >12 publications with GAD2 and Nervous System Diseases
 Brain Diseases Antibodies  >10 publications with GAD2 and Brain Diseases
 Schizophrenia Antibodies  >9 publications with GAD2 and Schizophrenia
 Epilepsy Antibodies  >6 publications with GAD2 and Epilepsy
 Seizures Antibodies  >6 publications with GAD2 and Seizures
 Inflammation Antibodies  >4 publications with GAD2 and Inflammation
 Depressive Disorder Antibodies  >3 publications with GAD2 and Depressive Disorder
 Movement Disorders Antibodies  >2 publications with GAD2 and Movement Disorders
 Substance Withdrawal Syndrome Antibodies  >2 publications with GAD2 and Substance Withdrawal Syndrome
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >22 publications with GAD2 and Brain
 Pancreas Antibodies  >2 publications with GAD2 and Pancreas
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