Antibody Protein ELISA Kit from MyBioSource
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KL elisa kit :: Mouse klotho ELISA Kit

Catalog #    MBS702609 KL elisa kit
Unit / Price
48 Strip Wells  /  $540 +1 FREE 8GB USB
96 Strip Wells  /  $775 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

klotho, ELISA Kit

 Also Known As   

Mouse Klotho ELISA Kit

 Product Synonym Names    Mouse Klotho ELISA Kit; klotho
 Product Gene Name   

KL elisa kit

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 KL elisa kit Manual   
 Immunogen Sequence Length    1014
 3D Structure    ModBase 3D Structure for O35082
 Species Reactivity    Mouse
 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse klotho. No significant cross-reactivity or interference between mouse klotho and analogues was observed.
 Samples    For the quantitative determination of mouse klotho concentrations in serum, plasma and cell culture supernates.
 Detection Range    3.12 pg/ml -200 pg/ml.
 Sensitivity    The minimum detectable dose of mouse klotho is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for KL elisa kit    Small volumes of KL elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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Product Description specifically for KL elisa kit

   Principle of the assay:
NCBI/Uniprot data below describe general gene information for KL. It may not necessarily be applicable to this product.
 NCBI GI #    227330584 [Other Products]
 NCBI GeneID    16591 [Other Products]
 NCBI Accession #    NP_038851.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013823.2 [Other Products]
 UniProt Primary Accession #    O35082 [Other Products]
 UniProt Secondary Accession #    O70175; O70621; B2RR78 [Other Products]
 UniProt Related Accession #    O35082 [Other Products]
 Molecular Weight    116,398 Da
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 NCBI Official Full Name    klotho
 NCBI Official Synonym Full Names    klotho
 NCBI Official Symbol    Kl [Similar Products]
 NCBI Official Synonym Symbols   

alpha-kl

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 NCBI Protein Information    klotho; secreted form of Klotho protein
 UniProt Protein Name    Klotho
 UniProt Gene Name    Kl [Similar Products]
 UniProt Entry Name    KLOT_MOUSE
 UniProt Comments for KL    Function: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.6 PublicationsManual assertion based on experiment in:Ref.1

Catalytic activity: A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.2 PublicationsManual assertion based on experiment in:Ref.13

Enzyme regulation: Inhibited by D-saccharic acid 1,4-lactone and taurocholic acid.1 PublicationManual assertion based on experiment in:Ref.13
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 UniProt References for KL    1. "Mutation of the mouse klotho gene leads to a syndrome resembling ageing."Kuro-o M., Matsumura Y., Aizawa H., Kawaguchi H., Suga T., Utsugi T., Ohyama Y., Kurabayashi M., Kaname T., Kume E., Iwasaki H., Iida A., Shiraki-Iida T., Nishikawa S., Nagai R., Nabeshima Y.Nature 390:45-51(1997) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION. Tissue: Kidney.
2. "Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein."Shiraki-Iida T., Aizawa H., Matsumura Y., Sekine S., Iida A., Anazawa H., Nagai R., Kuro-o M., Nabeshima Y.FEBS Lett. 424:6-10(1998) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
4. "Establishment of the anti-Klotho monoclonal antibodies and detection of Klotho protein in kidneys."Kato Y., Arakawa E., Kinoshita S., Shirai A., Furuya A., Yamano K., Nakamura K., Iida A., Anazawa H., Koh N., Iwano A., Imura A., Fujimori T., Kuro-o M., Hanai N., Takeshige K., Nabeshima Y.Biochem. Biophys. Res. Commun. 267:597-602(2000) [PubMed] [Europe PMC] [Abstract]. Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
5. "Disruption of klotho gene causes an abnormal energy homeostasis in mice."Mori K., Yahata K., Mukoyama M., Suganami T., Makino H., Nagae T., Masuzaki H., Ogawa Y., Sugawara A., Nabeshima Y., Nakao K.Biochem. Biophys. Res. Commun. 278:665-670(2000) [PubMed] [Europe PMC] [Abstract]. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
 Research Articles on KL    1. The presence of Klotho-independent FGF23 effects in a Klotho-expressing target organ represents a paradigm shift in the conceptualization of FGF23 endocrine action
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 Searchable Name for KL    anti-KL; Buy KL elisa kit ($540), Mouse klotho ELISA Kit (MBS702609) product datasheet at MyBioSource, ELISA Kits; 16591; KLOT_MOUSE; KL elisa kit; anti-KL
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with KL elisa kitPathways associated with KL elisa kit
 Reference Product  PubMed Publications
 Fgf23 elisa kit  >36 publications with KL and Fgf23
 Fgf15 elisa kit  >1 publications with KL and Fgf15
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
Diseases associated with KL elisa kitOrgans/Tissues associated with KL elisa kit
 Disease Name  Pubmed Publications
 Pneumonia Antibodies  >206 publications with KL and Pneumonia
 Fibrosis Antibodies  >142 publications with KL and Fibrosis
 Skin Diseases Antibodies  >118 publications with KL and Skin Diseases
 Pulmonary Fibrosis Antibodies  >116 publications with KL and Pulmonary Fibrosis
 Inflammation Antibodies  >89 publications with KL and Inflammation
 Heart Diseases Antibodies  >73 publications with KL and Heart Diseases
 Lung Injury Antibodies  >59 publications with KL and Lung Injury
 Kidney Diseases Antibodies  >55 publications with KL and Kidney Diseases
 Necrosis Antibodies  >43 publications with KL and Necrosis
 Hypertension Antibodies  >36 publications with KL and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >377 publications with KL and Lung
 Bone Antibodies  >243 publications with KL and Bone
 Brain Antibodies  >196 publications with KL and Brain
 Kidney Antibodies  >93 publications with KL and Kidney
 Connective Tissue Antibodies  >51 publications with KL and Connective Tissue
 Eye Antibodies  >32 publications with KL and Eye
 Spleen Antibodies  >31 publications with KL and Spleen
 Intestine Antibodies  >19 publications with KL and Intestine
 Parathyroid Antibodies  >16 publications with KL and Parathyroid
 Ganglia Antibodies  >13 publications with KL and Ganglia
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