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KL elisa kit :: Klotho [ELISA Kit]
Catalog #    MBS702609
Unit / Price
48 Strip Wells  /  $585.00 + 1 FREE 8GB USB Flash Drive
96 Strip Wells  /  $775.00 + 1 FREE 8GB USB Flash Drive
    
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    Klotho, ELISA Kit
 Also Known As    Mouse Klotho ELISA Kit
 Product Synonym Names    klotho; N/A
 Product Gene Name    KL elisa kit [Similar Products]
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 KL elisa kit Manual   
 Immunogen Sequence Length    1012
 Chromosome Location    Chromosome: 13; NC_000013.10 (33590571..33640282). Location: 13q12
 OMIM    211900
 3D Structure    ModBase 3D Structure for O35082
 Species Reactivity    Mouse
 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse klotho. No significant cross-reactivity or interference between mouse klotho and analogues was observed.
 Samples    For the quantitative determination of mouse klotho concentrations in serum, plasma and cell culture supernates.
 Detection Range    3.12 pg/ml-200 pg/ml.
 Sensitivity    The minimum detectable dose of mouse klotho is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for KL elisa kit    Small volumes of KL elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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 Product Description specifically for KL elisa kit    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for klotho has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any klotho present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for klotho is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of klotho bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for KL. It may not necessarily be applicable to this product.
 NCBI GI #    2618596 [Other Products]
 NCBI GeneID    9365 [Other Products]
 NCBI Accession #    NP_038851.2 [Other Products]
 UniProt Primary Accession #    O35082 [Other Products]
 UniProt Secondary Accession #    O70175; O70621; B2RR78 [Other Products]
 UniProt Related Accession #    Q9UEF7 [Other Products]
 Molecular Weight    116,181 Da
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 NCBI Official Full Name    klotho
 NCBI Official Synonym Full Names    klotho
 NCBI Official Symbol    KL [Similar Products]
 NCBI Protein Information    klotho
 UniProt Protein Name    Klotho
 UniProt Gene Name    Kl [Similar Products]
 UniProt Entry Name    KLOT_MOUSE
 NCBI Summary for KL    This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]
 UniProt Comments for KL    Function: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Ref.1 Ref.5 Ref.6 Ref.9 Ref.14 Ref.15The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. Ref.1 Ref.5 Ref.6 Ref.9 Ref.14 Ref.15

Catalytic activity: A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol. Ref.13 Ref.14

Enzyme regulation: Inhibited by D-saccharic acid 1,4-lactone and taurocholic acid. Ref.13

Subunit structure: Homodimer. Interacts with FGF23 and FGFR1. Ref.12 Ref.15

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note: Isoform 1 shedding leads to a soluble peptide. Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14Isoform 2: Secreted Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14. Klotho peptide: Secreted Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14.

Tissue specificity: Membrane-bound protein is present in distal renal tubules, inner ear, ependymal cells of brain choroid plexus, elongating spermatids and mature oocytes (at protein level). Soluble peptide is present in serum (100 pM) and cerebrospinal fluid. Expressed strongly in kidney, moderately in brain choroid plexus, and at low levels in pituitary, placenta, skeletal muscle, urinary bladder, aorta, pancreas, testis, ovary, colon, thyroid gland and adipocytes. Ref.1 Ref.2 Ref.4 Ref.7 Ref.8 Ref.11 Ref.12 Ref.14

Developmental stage: Not expressed in the embryo. Expressed in the kidney of newborns. Ref.1

Induction: Induced by 1,25-dihydroxyvitamin D3 in kidney. Down-regulated by angiotensin II and up-regulated by statins through modulation of the RhoA pathway in epithelial cells (in vitro). Isoform 1 (but not isoform 2) is up-regulated by thyroid hormone in adipocytes. Ref.7 Ref.9 Ref.10 Ref.13

Domain: Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 241, and the second one lacks the essential Glu active site residue at position 874.

Post-translational modification: N-glycosylated. Ref.12

Disruption phenotype: Mice display a syndrome resembling to human aging, with short lifespan, infertility, atherosclerosis, skin atrophy, osteoporosis and emphysema. They have various metabolic abnormalities, including increased insulin sensitivity and decreased insulin production. Mice overexpressing Kl have increased resistance to insulin and IGF1, a lifespan extended of more than 20%, and generate fewer offspring. Ref.5

Sequence similarities: Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Biophysicochemical propertiesKinetic parameters:KM=0.249 mM for 4-methylumbelliferylglucuronide Ref.13KM=0.251 mM for estrone 3-beta-D-glucuronideKM=0.174 mM for beta-estradiol 3-beta-D-glucuronideKM=0.251 mM for estriol 3-beta-D-glucuronideVmax=0.62 µM/h/µg enzymepH dependence:Optimum pH is 5.5.
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 NCBI References for KL    1. Soluble α-klotho: a novel serum biomarker for the activity of GH-producing pituitary adenomas.
2. FGF23 suppresses chondrocyte proliferation in the presence of soluble α-Klotho both in vitro and in vivo.
3. Inhibition of lung cancer cells growth, motility and induction of apoptosis by Klotho, a novel secreted Wnt antagonist, in a dose-dependent manner.
4. Soluble serum Klotho in diabetic nephropathy: relationship to VEGF-A.
5. Promoter methylation confers kidney-specific expression of the Klotho gene.
 UniProt References for KL    • Mutation of the mouse klotho gene leads to a syndrome resembling ageing. Kuro-o M., Matsumura Y., Aizawa H., Kawaguchi H., Suga T., Utsugi T., Ohyama Y., Kurabayashi M., Kaname T., Kume E., Iwasaki H., Iida A., Shiraki-Iida T., Nishikawa S., Nagai R., Nabeshima Y.Nature 390:45-51(1997) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION. Tissue: Kidney.
• Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein. Shiraki-Iida T., Aizawa H., Matsumura Y., Sekine S., Iida A., Anazawa H., Nagai R., Kuro-o M., Nabeshima Y.FEBS Lett. 424:6-10(1998) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
• Establishment of the anti-Klotho monoclonal antibodies and detection of Klotho protein in kidneys. Kato Y., Arakawa E., Kinoshita S., Shirai A., Furuya A., Yamano K., Nakamura K., Iida A., Anazawa H., Koh N., Iwano A., Imura A., Fujimori T., Kuro-o M., Hanai N., Takeshige K., Nabeshima Y.Biochem. Biophys. Res. Commun. 267:597-602(2000) [PubMed] [Europe PMC] [Abstract]Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
• Disruption of klotho gene causes an abnormal energy homeostasis in mice. Mori K., Yahata K., Mukoyama M., Suganami T., Makino H., Nagae T., Masuzaki H., Ogawa Y., Sugawara A., Nabeshima Y., Nakao K.Biochem. Biophys. Res. Commun. 278:665-670(2000) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, DISRUPTION PHENOTYPE.
 Research Articles on KL    1. In diabetic patients, Klotho is decreased in early chronic kidney disease and increases thereafter, paralleling reduced glomerular filtration rate.
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 Searchable Name for KL    anti-KL; Buy KL elisa kit :: Klotho, ELISA Kit (MBS702609) datasheet at MyBioSource; anti-KL; KL; anti-Klotho; Mouse Klotho ELISA Kit; klotho; N/A; klotho; klotho; Klotho anti-Mouse. (NCBI/UniProt 2618596 NP_038851.2 Q9UEF7 O35082 O70175; O70621; B2RR78 116,181 Da); Chromosome: 13; NC_000013.10 (33590571..33640282). Location: 13q12; 9365; KLOT_MOUSE; KL elisa kit; anti-KL
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with KL elisa kitPathways associated with KL elisa kit
 Product Name  Ref Prod  PubMed Publications
 KL elisa kit  IGF1R elisa kit  >4 publications with KL and IGF1R
 Product Name  Products by Pathway  Pathway Diagram
 KL elisa kit  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 KL elisa kit  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 KL elisa kit  Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 KL elisa kit  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
 KL elisa kit  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
 KL elisa kit  FGFR Ligand Binding And Activation Pathway antibodies  FGFR Ligand Binding And Activation Pathway Diagram
 KL elisa kit  FGFR1c And Klotho Ligand Binding And Activation Pathway antibodies  FGFR1c And Klotho Ligand Binding And Activation Pathway Diagram
 KL elisa kit  FRS2-mediated Cascade Pathway antibodies  FRS2-mediated Cascade Pathway Diagram
 KL elisa kit  IRS-mediated Signalling Pathway antibodies  IRS-mediated Signalling Pathway Diagram
 KL elisa kit  IRS-related Events Pathway antibodies  IRS-related Events Pathway Diagram
Diseases associated with KL elisa kitOrgans/Tissues associated with KL elisa kit
 Disease Name  Ref Prod  Pubmed Publications
 Lung Diseases Antibodies  anti-KL  >357 publications with KL and Lung Diseases
 Pneumonia Antibodies  anti-KL  >176 publications with KL and Pneumonia
 Fibrosis Antibodies  anti-KL  >116 publications with KL and Fibrosis
 Skin Diseases Antibodies  anti-KL  >103 publications with KL and Skin Diseases
 Pulmonary Fibrosis Antibodies  anti-KL  >97 publications with KL and Pulmonary Fibrosis
 Infection Antibodies  anti-KL  >97 publications with KL and Infection
 Heart Diseases Antibodies  anti-KL  >69 publications with KL and Heart Diseases
 Inflammation Antibodies  anti-KL  >69 publications with KL and Inflammation
 Adenocarcinoma Antibodies  anti-KL  >64 publications with KL and Adenocarcinoma
 Lung Neoplasms Antibodies  anti-KL  >61 publications with KL and Lung Neoplasms
 Organ/Tissue Name  Ref Prod  Pubmed Publications
 Lung Antibodies  KL antibody  >377 publications with KL and Lung
 Bone Antibodies  KL antibody  >243 publications with KL and Bone
 Brain Antibodies  KL antibody  >196 publications with KL and Brain
 Kidney Antibodies  KL antibody  >93 publications with KL and Kidney
 Connective Tissue Antibodies  KL antibody  >51 publications with KL and Connective Tissue
 Eye Antibodies  KL antibody  >32 publications with KL and Eye
 Spleen Antibodies  KL antibody  >31 publications with KL and Spleen
 Intestine Antibodies  KL antibody  >19 publications with KL and Intestine
 Parathyroid Antibodies  KL antibody  >16 publications with KL and Parathyroid
 Ganglia Antibodies  KL antibody  >13 publications with KL and Ganglia
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