Antibody Protein ELISA Kit from MyBioSource 1.858.633.0165          
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KL elisa kit :: Mouse klotho ELISA Kit

Catalog #    MBS702609 KL elisa kit
Unit / Price
48 Strip Wells  /  $540 +1 FREE 8GB USB
96 Strip Wells  /  $775 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

klotho, ELISA Kit

 Also Known As   

Mouse Klotho ELISA Kit

 Product Synonym Names    Mouse Klotho ELISA Kit; klotho
 Product Gene Name   

KL elisa kit

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 KL elisa kit Manual   
 Immunogen Sequence Length    1014
 3D Structure    ModBase 3D Structure for O35082
 Species Reactivity    Mouse
 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse klotho. No significant cross-reactivity or interference between mouse klotho and analogues was observed.
 Samples    For the quantitative determination of mouse klotho concentrations in serum, plasma and cell culture supernates.
 Detection Range    3.12 pg/ml -200 pg/ml.
 Sensitivity    The minimum detectable dose of mouse klotho is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Other Notes for KL elisa kit    Small volumes of KL elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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Product Description specifically for KL elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for klotho has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any klotho present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for klotho is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of klotho bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for KL. It may not necessarily be applicable to this product.
 NCBI GI #    227330584 [Other Products]
 NCBI GeneID    16591 [Other Products]
 NCBI Accession #    NP_038851.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013823.2 [Other Products]
 UniProt Primary Accession #    O35082 [Other Products]
 UniProt Secondary Accession #    O70175; O70621; B2RR78 [Other Products]
 UniProt Related Accession #    O35082 [Other Products]
 Molecular Weight    116,398 Da
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 NCBI Official Full Name    klotho
 NCBI Official Synonym Full Names    klotho
 NCBI Official Symbol    Kl [Similar Products]
 NCBI Official Synonym Symbols   

alpha-kl

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 NCBI Protein Information    klotho; secreted form of Klotho protein
 UniProt Protein Name    Klotho
 UniProt Gene Name    Kl [Similar Products]
 UniProt Entry Name    KLOT_MOUSE
 UniProt Comments for KL    Function: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.6 PublicationsManual assertion based on experiment in:Ref.1

Catalytic activity: A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.2 PublicationsManual assertion based on experiment in:Ref.13

Enzyme regulation: Inhibited by D-saccharic acid 1,4-lactone and taurocholic acid.1 PublicationManual assertion based on experiment in:Ref.13
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 UniProt References for KL    1. "Mutation of the mouse klotho gene leads to a syndrome resembling ageing."Kuro-o M., Matsumura Y., Aizawa H., Kawaguchi H., Suga T., Utsugi T., Ohyama Y., Kurabayashi M., Kaname T., Kume E., Iwasaki H., Iida A., Shiraki-Iida T., Nishikawa S., Nagai R., Nabeshima Y.Nature 390:45-51(1997) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION. Tissue: Kidney.
2. "Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein."Shiraki-Iida T., Aizawa H., Matsumura Y., Sekine S., Iida A., Anazawa H., Nagai R., Kuro-o M., Nabeshima Y.FEBS Lett. 424:6-10(1998) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
4. "Establishment of the anti-Klotho monoclonal antibodies and detection of Klotho protein in kidneys."Kato Y., Arakawa E., Kinoshita S., Shirai A., Furuya A., Yamano K., Nakamura K., Iida A., Anazawa H., Koh N., Iwano A., Imura A., Fujimori T., Kuro-o M., Hanai N., Takeshige K., Nabeshima Y.Biochem. Biophys. Res. Commun. 267:597-602(2000) [PubMed] [Europe PMC] [Abstract]. Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
5. "Disruption of klotho gene causes an abnormal energy homeostasis in mice."Mori K., Yahata K., Mukoyama M., Suganami T., Makino H., Nagae T., Masuzaki H., Ogawa Y., Sugawara A., Nabeshima Y., Nakao K.Biochem. Biophys. Res. Commun. 278:665-670(2000) [PubMed] [Europe PMC] [Abstract]. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
 Research Articles on KL    1. The presence of Klotho-independent FGF23 effects in a Klotho-expressing target organ represents a paradigm shift in the conceptualization of FGF23 endocrine action
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 Searchable Name for KL    anti-KL; Buy KL elisa kit, Mouse klotho ELISA Kit (MBS702609) product datasheet at MyBioSource, ELISA Kits; 16591; KLOT_MOUSE; KL elisa kit; anti-KL
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with KL elisa kitPathways associated with KL elisa kit
 Reference Product  PubMed Publications
 Fgf23 elisa kit  >36 publications with KL and Fgf23
 Fgf15 elisa kit  >1 publications with KL and Fgf15
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
Diseases associated with KL elisa kitOrgans/Tissues associated with KL elisa kit
 Disease Name  Pubmed Publications
 Pneumonia Antibodies  >206 publications with KL and Pneumonia
 Fibrosis Antibodies  >142 publications with KL and Fibrosis
 Skin Diseases Antibodies  >118 publications with KL and Skin Diseases
 Pulmonary Fibrosis Antibodies  >116 publications with KL and Pulmonary Fibrosis
 Inflammation Antibodies  >89 publications with KL and Inflammation
 Heart Diseases Antibodies  >73 publications with KL and Heart Diseases
 Lung Injury Antibodies  >59 publications with KL and Lung Injury
 Kidney Diseases Antibodies  >55 publications with KL and Kidney Diseases
 Necrosis Antibodies  >43 publications with KL and Necrosis
 Hypertension Antibodies  >36 publications with KL and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >377 publications with KL and Lung
 Bone Antibodies  >243 publications with KL and Bone
 Brain Antibodies  >196 publications with KL and Brain
 Kidney Antibodies  >93 publications with KL and Kidney
 Connective Tissue Antibodies  >51 publications with KL and Connective Tissue
 Eye Antibodies  >32 publications with KL and Eye
 Spleen Antibodies  >31 publications with KL and Spleen
 Intestine Antibodies  >19 publications with KL and Intestine
 Parathyroid Antibodies  >16 publications with KL and Parathyroid
 Ganglia Antibodies  >13 publications with KL and Ganglia
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