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PCT elisa kit :: Procalcitonin, PCT [ELISA Kit]
Catalog #    MBS702611
Unit / Price
48 Strip Wells  /  $630.00 +1 FREE 8GB USB
96 Strip Wells  /  $910.00 +1 FREE 8GB USB
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    Procalcitonin, PCT, ELISA Kit
 Also Known As    Mouse Procalcitonin, PCT ELISA Kit
 Product Synonym Names    Mouse Procalcitonin; PCT ELISA Kit; Procalcitonin; PCT
 Product Gene Name    PCT elisa kit [Similar Products]
 PCT elisa kit Manual   
 Immunogen Sequence Length    648
 3D Structure    ModBase 3D Structure for F7ZLI1
 Species Reactivity    Mouse
 Sample Type    serum, plasma, cell culture supernates, tissue homogenates
 Detection Range    1.56 pg/ml-100 pg/ml
 Detection Wavelength    450 nm
 Sensitivity    0.39 pg/ml
 Sample Volume    50-100ul
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for PCT elisa kit    Small volumes of PCT elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
 Product Description specifically for PCT elisa kit    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCT bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for PCT. It may not necessarily be applicable to this product.
 NCBI GI #    339502384 [Other Products]
 NCBI GeneID    10949209 [Other Products]
 NCBI Accession #    YP_004689804.1 [Other Products]
 NCBI GenBank Nucleotide #    NC_015730.1 [Other Products]
 UniProt Primary Accession #    F7ZLI1 [Other Products]
 Molecular Weight    69,733 Da
 NCBI Official Full Name    propionate CoA-transferase Pct
 NCBI Official Symbol    pct [Similar Products]
 NCBI Protein Information    propionate CoA-transferase Pct
 UniProt Protein Name    Propionate CoA-transferase Pct
 UniProt Synonym Protein Names    Propionate CoA-transferase Pct
 UniProt Gene Name    pct [Similar Products]
 UniProt Synonym Gene Names    RLO149_c008140 [Similar Products]
 UniProt Entry Name    F7ZLI1_ROSLO
 UniProt References for PCT    1. "Comparative genome analysis and genome-guided physiological analysis of Roseobacter litoralis."Kalhoefer D., Thole S., Voget S., Lehmann R., Liesegang H., Wollher A., Daniel R., Simon M., Brinkhoff T.BMC Genomics 12:324-324(2011) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49566 / DSM 6996 / JCM 21268 / NBRC 15278 / OCh 149. <p>Provides general information on the entry.</p><p><a href='../manual/entry_information_section' target='_top'>More...</a></p>Entry informationi<p>Provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.</p><p><a href='../manual/entry_name' target='_top'>More...</a></p>Entry nameiF7ZLI1_ROSLO<p>Provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.</p><p><a href='../manual/accession_numbers' target='_top'>More...</a></p>AccessioniF7ZLI1Primary (citable) accession number: F7ZLI1<p>Shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the sequence are also displayed.</p><p><a href='../manual/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: September 21, 2011Last sequence update: September 21, 2011Last modified: September 3, 2014This is version 13 of the entry and version 1 of the sequence. [Complete history]<p>Indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).</p><p><a href='../manual/entry_status' target='_top'>More...</a></p>Entry statusiUnrevi
 Searchable Name for PCT    anti-PCT; Buy PCT elisa kit :: Procalcitonin, PCT, ELISA Kit (MBS702611) datasheet at MyBioSource; anti-PCT; RLO149_c008140; anti-Procalcitonin, PCT; Mouse Procalcitonin, PCT ELISA Kit; Mouse Procalcitonin; PCT ELISA Kit; Procalcitonin; PCT; propionate CoA-transferase Pct; Propionate CoA-transferase Pct; Propionate CoA-transferase Pct anti-Mouse. (NCBI/UniProt 339502384 YP_004689804.1 NC_015730.1 F7ZLI1 69,733 Da); 10949209; F7ZLI1_ROSLO; PCT elisa kit; anti-PCT
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with PCT and Bone
 Connective Tissue Antibodies  >4 publications with PCT and Connective Tissue
 Heart Antibodies  >3 publications with PCT and Heart
 Muscle Antibodies  >3 publications with PCT and Muscle
 Brain Antibodies  >3 publications with PCT and Brain
 Vascular Antibodies  >2 publications with PCT and Vascular
 Embryonic Tissue Antibodies  >2 publications with PCT and Embryonic Tissue
 Ear Antibodies  >1 publications with PCT and Ear
 Kidney Antibodies  >1 publications with PCT and Kidney
 Umbilical Cord Antibodies  >1 publications with PCT and Umbilical Cord