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PCT elisa kit :: Procalcitonin, PCT [ELISA Kit]
Catalog #    MBS702611
Unit / Price
48 Strip Wells  /  $675.00 + 1 FREE 8GB USB Flash Drive
96 Strip Wells  /  $930.00 + 1 FREE 8GB USB Flash Drive
    
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    Procalcitonin, PCT, ELISA Kit
 Also Known As    Mouse Procalcitonin, PCT ELISA Kit
 Product Synonym Names    Procalcitonin, PCT; N/A
 Product Gene Name    PCT elisa kit [Similar Products]
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 PCT elisa kit Manual   
 Chromosome Location    NC_015730.1 (876880..878826). Location: isolation source: seaweed
 Species Reactivity    Mouse
 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse PCT. No significant cross-reactivity or interference between mouse PCT and analogues was observed.
 Samples    For the quantitative determination of mouse procalcitonin (PCT) concentrations in serum, plasma, cell culture supernates, tissue homogenates.
 Detection Range    1.56 pg/ml-100 pg/ml.
 Samples    For the quantitative determination of mouse procalcitonin (PCT) concentrations in serum, plasma, cell culture supernates, tissue homogenates.
 Detection Range    1.56 pg/ml-100 pg/ml.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for PCT elisa kit    Small volumes of PCT elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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 Product Description specifically for PCT elisa kit    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCT bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for PCT. It may not necessarily be applicable to this product.
 NCBI GI #    339502384 [Other Products]
 NCBI GeneID    10949209 [Other Products]
 NCBI Accession #    YP_004689804.1 [Other Products]
 NCBI GenBank Nucleotide #    NC_015730.1 [Other Products]
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 NCBI Official Full Name    propionate CoA-transferase Pct
 NCBI Official Symbol    pct [Similar Products]
 NCBI Protein Information    propionate CoA-transferase Pct
 NCBI References for PCT    1. Comparative genome analysis and genome-guided physiological analysis of Roseobacter litoralis.
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 Searchable Name for PCT    anti-PCT; Buy PCT elisa kit :: Procalcitonin, PCT, ELISA Kit (MBS702611) datasheet at MyBioSource; anti-PCT; PCT; anti-Procalcitonin, PCT; Mouse Procalcitonin, PCT ELISA Kit; Procalcitonin, PCT; N/A; propionate CoA-transferase Pct anti-Mouse. (NCBI/UniProt 339502384 YP_004689804.1 NC_015730.1); NC_015730.1 (876880..878826). Location: isolation source: seaweed; 10949209; PCT elisa kit; anti-PCT
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products similar to Mouse Procalcitonin, PCT ELISA Kit...
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 MBS295110  Mouse Procalcitonin, PCT ELISA Kit (ELISA Kit)  MBS160843  Mouse Procalcitonin, PCT ELISA Kit (ELISA Kit)
Pathways associated with PCT elisa kitOrgans/Tissues associated with PCT elisa kit
 Product Name  Products by Pathway  Pathway Diagram
 PCT elisa kit  Microbial Metabolism In Diverse Environments Pathway antibodies  Microbial Metabolism In Diverse Environments Pathway Diagram
 PCT elisa kit  Propanoate Metabolism Pathway antibodies  Propanoate Metabolism Pathway Diagram
 PCT elisa kit  Propanoate Metabolism Pathway antibodies  Propanoate Metabolism Pathway Diagram
 PCT elisa kit  Pyruvate Metabolism Pathway antibodies  Pyruvate Metabolism Pathway Diagram
 PCT elisa kit  Pyruvate Metabolism Pathway antibodies  Pyruvate Metabolism Pathway Diagram
 PCT elisa kit  Styrene Degradation Pathway antibodies  Styrene Degradation Pathway Diagram
 PCT elisa kit  Styrene Degradation Pathway antibodies  Styrene Degradation Pathway Diagram
 Organ/Tissue Name  Ref Prod  Pubmed Publications
 Bone Antibodies  PCT antibody  >13 publications with PCT and Bone
 Connective Tissue Antibodies  PCT antibody  >4 publications with PCT and Connective Tissue
 Heart Antibodies  PCT antibody  >3 publications with PCT and Heart
 Muscle Antibodies  PCT antibody  >3 publications with PCT and Muscle
 Brain Antibodies  PCT antibody  >3 publications with PCT and Brain
 Vascular Antibodies  PCT antibody  >2 publications with PCT and Vascular
 Embryonic Tissue Antibodies  PCT antibody  >2 publications with PCT and Embryonic Tissue
 Ear Antibodies  PCT antibody  >1 publications with PCT and Ear
 Kidney Antibodies  PCT antibody  >1 publications with PCT and Kidney
 Umbilical Cord Antibodies  PCT antibody  >1 publications with PCT and Umbilical Cord
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