Antibody Protein ELISA Kit from MyBioSource
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DBH elisa kit :: Human dopamine beta-hydroxylase (dopamine beta-monooxygenase) ELISA Kit

Catalog #    MBS703682 DBH elisa kit
Unit / Price
48 Strip Wells  /  $600 +1 FREE 8GB USB
96 Strip Wells  /  $865 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

dopamine beta-hydroxylase (dopamine beta-monooxygenase) (DBH), ELISA Kit

 Also Known As   

Human dopamine-beta-hydroxylase, DBH ELISA Kit

 Product Synonym Names    Human dopamine-beta-hydroxylase; DBH ELISA Kit; RP11-317B10.1; DBM; dopamine beta hydroxylase; dopamine beta-hydroxylase (dopamine beta-monooxygenase)
 Product Gene Name   

DBH elisa kit

[Similar Products]
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 DBH elisa kit Manual   
 Immunogen Sequence Length    617
 OMIM    phenotype 609312
 3D Structure    ModBase 3D Structure for P09172
 Species Reactivity    Human
 Specificity    This assay has high sensitivity and excellent specificity for detection of human DBH. No significant cross-reactivity or interference between human DBH and analogues was observed.
 Samples    For the quantitative determination of human dopamine-?-hydroxylase (DBH) concentrations in serum, plasma, tissue homogenates.
 Detection Range    2.5 ng/ml -500 ng/ml.
 Sensitivity    The minimum detectable dose of human DBH is typically less than 1.25 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human DBH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for DBH elisa kit    Small volumes of DBH elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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Product Description specifically for DBH elisa kit

   Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for DBH and Horseradish Peroxidase (HRP) conjugated DBH. The competitive inhibition reaction is launched between with HRP labeled DBH and unlabeled DBH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of DBH in the sample. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for DBH. It may not necessarily be applicable to this product.
 NCBI GI #    116534900 [Other Products]
 NCBI GeneID    1621 [Other Products]
 NCBI Accession #    NP_000778.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000787.3 [Other Products]
 UniProt Primary Accession #    P09172 [Other Products]
 UniProt Secondary Accession #    Q5T381; Q96AG2 [Other Products]
 UniProt Related Accession #    P09172 [Other Products]
 Molecular Weight    69,065 Da
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 NCBI Official Full Name    dopamine beta-hydroxylase
 NCBI Official Synonym Full Names    dopamine beta-hydroxylase (dopamine beta-monooxygenase)
 NCBI Official Symbol    DBH [Similar Products]
 NCBI Official Synonym Symbols   

DBM

[Similar Products]
 NCBI Protein Information    dopamine beta-hydroxylase
 UniProt Protein Name    Dopamine beta-hydroxylase
 UniProt Synonym Protein Names   

Dopamine beta-monooxygenaseCleaved into the following chain:Soluble dopamine beta-hydroxylase

 UniProt Gene Name    DBH [Similar Products]
 UniProt Entry Name    DOPO_HUMAN
 NCBI Summary for DBH    The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008]
 UniProt Comments for DBH    Function: Conversion of dopamine to noradrenaline.

Catalytic activity: 3,4-dihydroxyphenethylamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O.

Cofactor: Binds 1 PQQ per subunit.
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 UniProt References for DBH    1. "DNA sequence and analysis of human chromosome 9."Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
2. "The primary structure of human dopamine-beta-hydroxylase: insights into the relationship between the soluble and the membrane-bound forms of the enzyme."Lamouroux A., Vigny A., Faucon Biguet N., Darmon M.C., Franck R., Henry J.-P., Mallet J.EMBO J. 6:3931-3937(1987) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-617, PARTIAL PROTEIN SEQUENCE, VARIANTS THR-211 AND CYS-549.
3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-617. Tissue: Brain.
4. "Human dopamine beta-hydroxylase gene: two mRNA types having different 3'-terminal regions are produced through alternative polyadenylation."Kobayashi K., Kurosawa Y., Fukita K., Nagatsu T.Nucleic Acids Res. 17:1089-1102(1989) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-617.
5. "Expression of human dopamine beta-hydroxylase in Drosophila Schneider 2 cells."Li B., Tsing S., Kosaka A.H., Nguyen B., Osen E.G., Bach C., Chan H., Barnett J.Biochem. J. 313:57-64(1996) [PubMed] [Europe PMC] [Abstract]. Cited for: PROTEIN SEQUENCE OF 40-48.
 Research Articles on DBH    1. role for DBH promoter variation in long-term renal decline rate
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 Searchable Name for DBH    anti-DBH; Buy DBH elisa kit ($600), Human dopamine beta-hydroxylase (dopamine beta-monooxygenase) ELISA Kit (MBS703682) product datasheet at MyBioSource, ELISA Kits; 1621; DOPO_HUMAN; DBH elisa kit; anti-DBH
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with DBH elisa kitPathways associated with DBH elisa kit
 Reference Product  PubMed Publications
 PNMT elisa kit  >187 publications with DBH and PNMT
 NPY elisa kit  >120 publications with DBH and NPY
 COMT elisa kit  >77 publications with DBH and COMT
 DDC elisa kit  >34 publications with DBH and DDC
 MAOA elisa kit  >26 publications with DBH and MAOA
 MAOB elisa kit  >5 publications with DBH and MAOB
 CYB561 elisa kit  >1 publications with DBH and CYB561
 Products by Pathway  Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Catecholamine Biosynthesis Pathway antibodies  Catecholamine Biosynthesis Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Monoamine Transport Pathway antibodies  Monoamine Transport Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
Diseases associated with DBH elisa kitOrgans/Tissues associated with DBH elisa kit
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >244 publications with DBH and Mental Disorders
 Nervous System Diseases Antibodies  >211 publications with DBH and Nervous System Diseases
 Hypertension Antibodies  >95 publications with DBH and Hypertension
 Movement Disorders Antibodies  >56 publications with DBH and Movement Disorders
 Hypotension Antibodies  >42 publications with DBH and Hypotension
 Pheochromocytoma Antibodies  >39 publications with DBH and Pheochromocytoma
 Depressive Disorder Antibodies  >38 publications with DBH and Depressive Disorder
 Bipolar Disorder Antibodies  >28 publications with DBH and Bipolar Disorder
 Parkinsonian Disorders Antibodies  >26 publications with DBH and Parkinsonian Disorders
 Neurotoxicity Syndromes Antibodies  >19 publications with DBH and Neurotoxicity Syndromes
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >504 publications with DBH and Brain
 Nerve Antibodies  >361 publications with DBH and Nerve
 Adrenal Gland Antibodies  >214 publications with DBH and Adrenal Gland
 Ganglia Antibodies  >149 publications with DBH and Ganglia
 Embryonic Tissue Antibodies  >26 publications with DBH and Embryonic Tissue
 Mouth Antibodies  >15 publications with DBH and Mouth
 Testis Antibodies  >7 publications with DBH and Testis
 Lymph Node Antibodies  >5 publications with DBH and Lymph Node
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