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NT-proBNP elisa kit :: Rat N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit

Catalog #    MBS704791 NT-proBNP elisa kit
Unit / Price
48 Strip Wells  /  $600 +1 FREE 8GB USB
96 Strip Wells  /  $865 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

N-terminal pro-brain natriuretic peptide, NT-proBNP, ELISA Kit

 Also Known As   

Rat N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit

 Product Synonym Names    Rat N-terminal pro-brain natriuretic peptide; NT-proBNP ELISA KIT; N-terminal pro-brain natriuretic peptide; NT-proBNP
 Product Gene Name   

NT-proBNP elisa kit

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 NT-proBNP elisa kit Manual   
 Species Reactivity    Rat
 Specificity    This assay has high sensitivity and excellent specificity for detection of rat NT-proBNP. No significant cross-reactivity or interference between rat NT-proBNP and analogues was observed.
 Samples    For the quantitative determination of rat N-terminal pro-brain natriuretic peptide (NT-proBNP) concentrations in serum, plasma and tissue homogenates.
 Detection Range    7.8 pg/ml -500 pg/ml.
 Sensitivity    The minimum detectable dose of rat NT-proBNP is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for NT-proBNP elisa kit    Small volumes of NT-proBNP elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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Product Description specifically for NT-proBNP elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NT-proBNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NT-proBNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NT-proBNP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NT-proBNP bound in the initial step. The color development is stopped and the intensity of the color is measured.
 Searchable Name for NT-proBNP    anti-NT-proBNP; Buy NT-proBNP elisa kit ($600), Rat N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit (MBS704791) product datasheet at MyBioSource, ELISA Kits; NT-proBNP elisa kit; anti-NT-proBNP
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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