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NT-proBNP elisa kit :: N-terminal pro-brain natriuretic peptide, NT-proBNP [ELISA Kit]
Catalog #    MBS704791
Unit / Price
48 Strip Wells  /  $645.00 + 1 FREE 8GB USB Flash Drive
96 Strip Wells  /  $880.00 + 1 FREE 8GB USB Flash Drive
    
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 Product Name    N-terminal pro-brain natriuretic peptide, NT-proBNP, ELISA Kit
 Also Known As    Rat N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit
 Product Synonym Names    N-terminal pro-brain natriuretic peptide, NT-proBNP
 Product Gene Name    NT-proBNP elisa kit [Similar Products]
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 NT-proBNP elisa kit Manual   
 Species Reactivity    Rat
 Samples    This immunoassay kit allows for the in vitro quantitative determination of rat NT-proBNP concentrations in serum, plasma.
 Detection Range    7.8 pg/ml-500 pg/ml. The standard curve concentrations used for the ELISA's were 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml, 7.8 pg/ml.
 Sensitivity    The minimum detectable dose of rat NT-proBNP is typically less than 2 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for NT-proBNP elisa kit    Small volumes of NT-proBNP elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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 Product Description specifically for NT-proBNP elisa kit    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NT-proBNP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NT-proBNP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NT-proBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NT-proBNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Searchable Name for NT-proBNP    anti-NT-proBNP; Buy NT-proBNP elisa kit :: N-terminal pro-brain natriuretic peptide, NT-proBNP, ELISA Kit (MBS704791) datasheet at MyBioSource; anti-NT-proBNP; NT-proBNP; anti-N-terminal pro-brain natriuretic peptide, NT-proBNP; Rat N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit; N-terminal pro-brain natriuretic peptide, NT-proBNP anti-Rat. (NCBI/UniProt ); NT-proBNP elisa kit; anti-NT-proBNP
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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