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NFkappaB p65 elisa kit :: nuclear factor-kappaB p65, NF-kappaB p65 [ELISA Kit]
Catalog #    MBS704916
Unit / Price
48 Strip Wells  /  $690.00 + 1 FREE 8GB USB Flash Drive
96 Strip Wells  /  $950.00 + 1 FREE 8GB USB Flash Drive
    
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    nuclear factor-kappaB p65, NF-kappaB p65, ELISA Kit
 Also Known As    Rat nuclear factor-kappaB p65, NF-kappaB p65 ELISA Kit
 Product Synonym Names    nuclear factor-kappaB p65, NF-kappaB p65; nuclear factor-kB p65, NF-kB p65; Rat nuclear factor-kB p65, NF-kB p65 ELISA Kit
 Product Gene Name    NFkappaB p65 elisa kit [Similar Products]
 Product Synonym Gene Name    NFkB p65 [Similar Products]
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 NFkappaB p65 elisa kit Manual   
 Species Reactivity    Rat
 Specificity    This assay has high sensitivity and excellent specificity for detection of rat NF-kappab p65. No significant cross-reactivity or interference between rat NF-kappaB p65 and analogues was observed.
 Samples    For the quantitative determination of rat nuclear factor-kappaB p65 (NF-kappaB p65) concentrations in serum, plasma, tissue homogenates.
 Detection Range    1.56 pg/ml-100 pg/ml.
 Sensitivity    The minimum detectable dose of rat NF-kappaB p65 is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for NFkappaB p65 elisa kit    Small volumes of NFkappaB p65 elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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 Product Description specifically for NFkappaB p65 elisa kit    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-kappaB p65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-kappaB p65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-kappaB p65 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-kappaB p65 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for NFkappaB p65. It may not necessarily be applicable to this product.
 NCBI GI #    117616548 [Other Products]
 NCBI Accession #    ABK42292.1 [Other Products]
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 NCBI Official Full Name    NFkappaB p65
 Searchable Name for NFkappaB p65    anti-NFkappaB p65; Buy NFkappaB p65 elisa kit :: nuclear factor-kappaB p65, NF-kappaB p65, ELISA Kit (MBS704916) datasheet at MyBioSource; anti-NFkappaB p65; NFkB p65; anti-nuclear factor-kappaB p65, NF-kappaB p65; Rat nuclear factor-kappaB p65, NF-kappaB p65 ELISA Kit; nuclear factor-kappaB p65, NF-kappaB p65; nuclear factor-kB p65, NF-kB p65; Rat nuclear factor-kB p65, NF-kB p65 ELISA Kit; NFkappaB p65 anti-Rat. (NCBI/UniProt 117616548 ABK42292.1); NFkappaB p65 elisa kit; anti-NFkappaB p65
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with NFkappaB p65 elisa kitPathways associated with NFkappaB p65 elisa kit
 Product Name  Ref Prod  PubMed Publications
 NFkappaB p65 elisa kit  FLNA elisa kit  >12 publications with NFkappaB p65 and FLNA
 Product Name  Products by Pathway  Pathway Diagram
 NFkappaB p65 elisa kit  Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 NFkappaB p65 elisa kit  Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 NFkappaB p65 elisa kit  MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 NFkappaB p65 elisa kit  MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 NFkappaB p65 elisa kit  Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 NFkappaB p65 elisa kit  Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with NFkappaB p65 elisa kit
 Organ/Tissue Name  Ref Prod  Pubmed Publications
 Bone Antibodies  NFkappaB p65 antibody  >13 publications with NFkappaB p65 and Bone
 Connective Tissue Antibodies  NFkappaB p65 antibody  >4 publications with NFkappaB p65 and Connective Tissue
 Heart Antibodies  NFkappaB p65 antibody  >3 publications with NFkappaB p65 and Heart
 Muscle Antibodies  NFkappaB p65 antibody  >3 publications with NFkappaB p65 and Muscle
 Brain Antibodies  NFkappaB p65 antibody  >3 publications with NFkappaB p65 and Brain
 Vascular Antibodies  NFkappaB p65 antibody  >2 publications with NFkappaB p65 and Vascular
 Embryonic Tissue Antibodies  NFkappaB p65 antibody  >2 publications with NFkappaB p65 and Embryonic Tissue
 Ear Antibodies  NFkappaB p65 antibody  >1 publications with NFkappaB p65 and Ear
 Kidney Antibodies  NFkappaB p65 antibody  >1 publications with NFkappaB p65 and Kidney
 Umbilical Cord Antibodies  NFkappaB p65 antibody  >1 publications with NFkappaB p65 and Umbilical Cord
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