Antibody Protein ELISA Kit from MyBioSource
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NTX elisa kit :: Human cross linked N-telopeptide of type I collagen, NTX ELISA Kit

Catalog #    MBS705111 NTX elisa kit
Unit / Price
48 Strip Wells  /  $540 +1 FREE 8GB USB
96 Strip Wells  /  $775 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

cross linked N-telopeptide of type I collagen, NTX, ELISA Kit

 Also Known As   

Human cross linked N-telopeptide of type I collagen, NTX ELISA Kit

 Product Synonym Names    Human cross linked N-telopeptide of type I collagen; NTX ELISA Kit; cross linked N-telopeptide of type I collagen; NTX
 Product Gene Name   

NTX elisa kit

[Similar Products]
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 NTX elisa kit Manual   
 Immunogen Sequence Length    1275
 3D Structure    ModBase 3D Structure for Q9QTG7
 Species Reactivity    Human
 Specificity    This assay has high sensitivity and excellent specificity for detection of human NTX. No significant cross-reactivity or interference between human NTX and analogues was observed.
 Samples    For the quantitative determination of human cross linked N-telopeptide of type I collagen (NTX) concentrations in serum, plasma and urine.
 Detection Range    7.8 nM BCE -500 nM BCE.
 Sensitivity    The minimum detectable dose of human NTX is typically less than 195nMBCE. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for NTX elisa kit    Small volumes of NTX elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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Product Description specifically for NTX elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NTX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NTX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NTX is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NTX bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for NTX. It may not necessarily be applicable to this product.
 NCBI GI #    4579738 [Other Products]
 NCBI Accession #    BAA75084.1 [Other Products]
 UniProt Primary Accession #    Q9QTG7 [Other Products]
 Molecular Weight    146,743 Da
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 NCBI Official Full Name    NTX, partial
 UniProt Protein Name    NTX
 UniProt Synonym Protein Names   

NTX

 UniProt Gene Name    ntx [Similar Products]
 UniProt Entry Name    Q9QTG7_CBDP
 UniProt References for NTX    1. "Molecular composition of the 16S toxin produced by a Clostridium botulinum type D strain, 1873."Nakajima H., Inoue K., Ikeda T., Fujinaga Y., Sunagawa H., Takeshi K., Ohyama T., Watanabe T., Inoue K., Oguma K.Microbiol. Immunol. 42:599-605(1998) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE. Strain: 1873ImportedAutomatic assertion inferred from database entries:EMBL:BAA75084.1. <p>Provides general information on the entry.</p><p><a href='../manual/entry_information_section' target='_top'>More...</a></p>Entry informationi<p>Provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.</p><p><a href='../manual/entry_name' target='_top'>More...</a></p>Entry nameiQ9QTG7_CBDP<p>Provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.</p><p><a href='../manual/accession_numbers' target='_top'>More...</a></p>AccessioniQ9QTG7Primary (citable) accession number: Q9QTG7<p>Shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the sequence are also displayed.</p><p><a href='../manual/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: May 1, 2000Last sequence update: May 1, 2000Last modified: May 14, 2014This is version 58 of the entry and version 1 of the sequence. [Complete history]<p>Indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).</p><p><a href='../manual/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)<p>Provides links to the UniProt Reference Clusters (<a href="/help/uniref">UniRef</a>). UniRef consists of clusters for UniProtKB sequences (including isoforms) and selected UniParc sequences, in order to obtain complete coverage of the sequence space at resolutions of 100%, 90% and 50% identity.</p><p><a href='../manual/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi100% Identity90% Identity50% IdentityEntryCluster member(s)OrganismsLengthCluster IDCluster nameSizeQ9QTG7P19321Q2WG46C5VU81Clostridium botulinumClostridium botulinum D phage (Clostridium botulinum D bacteriophage)Clostridium botulinum D str. 18731,276UniRef100_P19321Cluster: Botulinum neurotoxin type D4Full viewEntryCluster member(s)OrganismsLengthCluster IDCluster nameSizeQ9QTG7P19321Q2WG46C5VU81Clostridium botulinumClostridium botulinum D phage (Clostridium botulinum D bacteriophage)Clostridium botulinum D str. 18731,276UniRef90_P19321Cluster: Botulinum neurotoxin type D4Full viewEntryCluster member(s)OrganismsLengthCluster IDCluster nameSizeQ9QTG7P18640Q642Y6Q66ND9F7MR19Q332E0Q93HT3Q7M2A3U6E8N1U6E8Q9+18Clostridium botulinumClostridium botulinum C phage (Clostridium botulinum C bacteriophage)Clostridium botulinum C str. StockholmClostridium botulinum phage CNClostridium botulinum C str. EklundClostridium botulinum BKT015925Clostridium botulinum CClostridium botulinum DClostridium botulinum D phage (Clostridium botulinum D bacteriophage)Clostridium botulinum D str. 18731,291UniRef50_P18640Cluster: Botulinum neurotoxin type C128
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 Searchable Name for NTX    anti-NTX; Buy NTX elisa kit ($540), Human cross linked N-telopeptide of type I collagen, NTX ELISA Kit (MBS705111) product datasheet at MyBioSource, ELISA Kits; Q9QTG7_CBDP; NTX elisa kit; anti-NTX
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with NTX elisa kitPathways associated with NTX elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with NTX and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with NTX elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with NTX and Bone
 Connective Tissue Antibodies  >4 publications with NTX and Connective Tissue
 Heart Antibodies  >3 publications with NTX and Heart
 Muscle Antibodies  >3 publications with NTX and Muscle
 Brain Antibodies  >3 publications with NTX and Brain
 Vascular Antibodies  >2 publications with NTX and Vascular
 Embryonic Tissue Antibodies  >2 publications with NTX and Embryonic Tissue
 Ear Antibodies  >1 publications with NTX and Ear
 Kidney Antibodies  >1 publications with NTX and Kidney
 Umbilical Cord Antibodies  >1 publications with NTX and Umbilical Cord
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