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HA elisa kit :: Hyaluronic Acid [ELISA Kit]
Catalog #    MBS727090
Unit / Price
48 Strip Wells  /  $545.00 + 1 FREE 8GB USB Flash Drive
96 Strip Wells  /  $800.00 + 1 FREE 8GB USB Flash Drive
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    Hyaluronic Acid, ELISA Kit
 Also Known As    Rat Hyaluronic Acid ELISA Kit
 Product Gene Name    HA elisa kit [Similar Products]
 HA elisa kit Manual   
 Species Reactivity    Rat
 Specificity    This assay has high sensitivity and excellent specificity for detection of HA. No significant cross-reactivity or interference between HA and analogues was observed. (NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HA and all the analogues, therefore, cross reaction may still exist in some cases.)
 Samples    Cell culture fluid & body fluid & tissue homogenate serum or blood plasma
 Sensitivity    The sensitivity in this assay is 1.0 ng/mL.
 Preparation and Storage    Store all reagents at 2-8 degree C
 Other Notes for HA elisa kit    Small volumes of HA elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
 Product Description specifically for HA elisa kit    Intended Uses: This HA ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of HA in the sample, this HA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus HA concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of HA utilizes a polyclonal anti-HA antibody and an HA-HRP conjugate. The assay sample and buffer are incubated together with HA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HA concentration since HA from samples and HA-HRP conjugate compete for the anti-HA antibody binding site. Since the number of sites is limited, as more sites are occupied by HA from the sample, fewer sites are left to bind HA-HRP conjugate.
 Product Categories/Family for HA elisa kit    Cell Biology
 Searchable Name for HA    anti-HA; Buy HA elisa kit :: Hyaluronic Acid, ELISA Kit (MBS727090) datasheet at MyBioSource; anti-HA; HA; anti-Hyaluronic Acid; Rat Hyaluronic Acid ELISA Kit anti-Rat. (NCBI/UniProt ); Cell Biology; HA elisa kit; anti-HA
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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