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HA elisa kit :: Rat Hyaluronic Acid ELISA Kit

Catalog #    MBS727090
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

Hyaluronic Acid (HA), ELISA Kit

 Also Known As   

Rat Hyaluronic Acid ELISA Kit

 Product Gene Name   

HA elisa kit

[Similar Products]
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 HA elisa kit Manual Insert   
 OMIM    605702
 Species Reactivity    Rat
 Specificity    This assay has high sensitivity and excellent specificity for detection of HA. No significant cross-reactivity or interference between HA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HA and all the analogues, therefore, cross reaction may still exist in some cases.
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive ELISA
 Sensitivity    1.0 ng/mL.
 Intended Uses    This HA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact technical support.
 Other Notes for HA elisa kit    Small volumes of HA elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HA purchase    anti-HA cost; Buy HA elisa kit, Rat Hyaluronic Acid ELISA Kit (MBS727090) product datasheet at MyBioSource, ELISA Kits; Cell Biology; 10894; LYVE1_HUMAN; HA elisa kit price; high sensitivity elisa kit for HA
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Product Description specifically for HA elisa kit

   Principle of the assay: HA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HA antibody and an HA-HRP conjugate. The assay sample and buffer are incubated together with HA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HA concentration since HA from samples and HA-HRP conjugate compete for the anti-HA antibody binding site. Since the number of sites is limited, as more sites are occupied by HA from the sample, fewer sites are left to bind HA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HA concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for HA elisa kit    Cell Biology
 Typical Testing Data/Standard Curve (for reference only) of HA elisa kit    HA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for HA. It may not necessarily be applicable to this product.
 NCBI GI #    10800122
 NCBI GeneID    10894
 NCBI Accession #    AAD49220.2 [Other Products]
 UniProt Secondary Accession #    Q8TC18; Q9UNF4 [Other Products]
 UniProt Related Accession #    Q9Y5Y7 [Other Products]
 Molecular Weight    35,213 Da
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 NCBI Official Full Name    hyaluronic acid receptor
 NCBI Official Synonym Full Names    lymphatic vessel endothelial hyaluronan receptor 1
 NCBI Official Symbol    LYVE1 [Similar Products]
 NCBI Official Synonym Symbols   

HAR; XLKD1; LYVE-1; CRSBP-1

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 NCBI Protein Information    lymphatic vessel endothelial hyaluronic acid receptor 1; extracellular link domain containing 1; extracellular link domain-containing 1; extracellular link domain-containing protein 1; cell surface retention sequence binding protein-1; cell surface retention sequence-binding protein 1
 UniProt Protein Name    Lymphatic vessel endothelial hyaluronic acid receptor 1
 UniProt Synonym Protein Names   

Cell surface retention sequence-binding protein 1; CRSBP-1; Extracellular link domain-containing protein 1; Hyaluronic acid receptor

 UniProt Gene Name    LYVE1 [Similar Products]
 UniProt Synonym Gene Names    CRSBP1; HAR; XLKD1; UNQ230/PRO263; LYVE-1; CRSBP-1 [Similar Products]
 UniProt Entry Name    LYVE1_HUMAN
 NCBI Summary for HA    This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. [provided by RefSeq, Jul 2008]
 UniProt Comments for HA    XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; hyaluronic acid binding; receptor activity

Biological Process: anatomical structure morphogenesis; glycosaminoglycan metabolic process; transport; cell-matrix adhesion; carbohydrate metabolic process; response to wounding; pathogenesis; signal transduction; cell motility; hyaluronan metabolic process; hyaluronan catabolic process
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 UniProt References for HA    1. "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-116. Tissue: Liver.
3. "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]. Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-53. Tissue: Plasma.
4. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]. Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-53. Tissue: Liver.
 Research Articles on HA    1. Data indicate that detection of lymphatic vascular invasion (LVI) can be optimized by specific D2-40 or LYVE-1 staining.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HA elisa kitPathways associated with HA elisa kit
 Reference Product  PubMed Publications
 VEGFC elisa kit  >59 publications with HA and VEGFC
 CD44 elisa kit  >19 publications with HA and CD44
 STAB2 elisa kit  >3 publications with HA and STAB2
 IGFBP3 elisa kit  >2 publications with HA and IGFBP3
 ATM elisa kit  >1 publications with HA and ATM
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 Hyaluronan Metabolism Pathway antibodies  Hyaluronan Metabolism Pathway Diagram
 Hyaluronan Uptake And Degradation Pathway antibodies  Hyaluronan Uptake And Degradation Pathway Diagram
 MPS I - Hurler Syndrome Pathway antibodies  MPS I - Hurler Syndrome Pathway Diagram
 MPS II - Hunter Syndrome Pathway antibodies  MPS II - Hunter Syndrome Pathway Diagram
 MPS IIIA - Sanfilippo Syndrome A Pathway antibodies  MPS IIIA - Sanfilippo Syndrome A Pathway Diagram
 MPS IIIB - Sanfilippo Syndrome B Pathway antibodies  MPS IIIB - Sanfilippo Syndrome B Pathway Diagram
 MPS IIIC - Sanfilippo Syndrome C Pathway antibodies  MPS IIIC - Sanfilippo Syndrome C Pathway Diagram
Diseases associated with HA elisa kitOrgans/Tissues associated with HA elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >21 publications with HA and Inflammation
 Breast Neoplasms Antibodies  >19 publications with HA and Breast Neoplasms
 Adenocarcinoma Antibodies  >18 publications with HA and Adenocarcinoma
 Disease Models, Animal Antibodies  >14 publications with HA and Disease Models, Animal
 Lung Neoplasms Antibodies  >13 publications with HA and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >13 publications with HA and Neoplasms, Experimental
 Lung Diseases Antibodies  >11 publications with HA and Lung Diseases
 Necrosis Antibodies  >10 publications with HA and Necrosis
 Liver Neoplasms Antibodies  >10 publications with HA and Liver Neoplasms
 Liver Diseases Antibodies  >9 publications with HA and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >174 publications with HA and Vascular
 Eye Antibodies  >22 publications with HA and Eye
 Lung Antibodies  >21 publications with HA and Lung
 Liver Antibodies  >15 publications with HA and Liver
 Embryonic Tissue Antibodies  >15 publications with HA and Embryonic Tissue
 Muscle Antibodies  >13 publications with HA and Muscle
 Stomach Antibodies  >10 publications with HA and Stomach
 Connective Tissue Antibodies  >9 publications with HA and Connective Tissue
 Bone Antibodies  >8 publications with HA and Bone
 Heart Antibodies  >7 publications with HA and Heart
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