Antibody Protein ELISA Kit from MyBioSource Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
Home Products Ordering Company Help
   MyBioSource   MBS729403 ELISA Kit / Human / Cardiac Troponin I / cTnI   LOG IN   MY ACCOUNT   CART CONTENTS   CHECKOUT 
MyBioSource Menu
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

 Advanced Search
 Submit Technical Q&A
 International Distributors
 Contact Us
Our Best Sellers more
 • Z-Dna Binding Protein (ZBP1) ELISA Kit
 • FABP Native Protein
 • Fatty Acid Binding Protein 5 Epidermal (FABP5) ELISA Kit
 • kallikrein-related peptidase 4 (KLK4) ELISA Kit
 • Ultrasensitivity Thyroxine (U-T4) ELISA Kit
 • Maltose transport system permease protein malF Recombinant Protein
 • TALIN-1 Antibody
 • Vitamin D3 Receptor (VDR) Antibody
 • A4GNT cDNA Clone
 • beta2 Microglobulin (beta2-MG) ELISA Kit
 • inhibin beta-B (Inhbb) ELISA Kit
 • Ubiquitin (RPS27A) Antibody
 • Insulin-1 (Ins1) Recombinant Protein
 • CD11c Antibody
  more ...

cTnI elisa kit :: Human Cardiac Troponin I ELISA Kit

Catalog #    MBS729403
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

Cardiac Troponin I (cTnI), ELISA Kit

 Also Known As   

Human Cardiac Troponin I ELISA Kit

 Product Gene Name   

cTnI elisa kit

[Similar Products]
TOP
 cTnI elisa kit Manual Insert   
 3D Structure    ModBase 3D Structure for Q6FGX2
 Species Reactivity    Human
 Assay Type    Sandwich ELISA
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact technical support.
 Other Notes for cTnI elisa kit    Small volumes of cTnI elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for cTnI purchase    anti-cTnI cost; Buy cTnI elisa kit, Human Cardiac Troponin I ELISA Kit (MBS729403) product datasheet at MyBioSource, ELISA Kits; Cardiovascular; 7137; Q6FGX2_HUMAN; cTnI elisa kit price; high sensitivity elisa kit for cTnI
TOP
 

Product Description specifically for cTnI elisa kit

   Intended Uses: This cTnI ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of cTnI in the sample, this cTnI ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus cTnI concentration. The concentration of cTnI in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This cTnI enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for cTnI. Standards or samples are then added to the microtiter plate wells and cTnI if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of cTnI present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for cTnI are added to each well to "sandwich" the cTnI immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain cTnI and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
 Product Categories/Family for cTnI elisa kit    Cardiovascular
 Typical Testing Data/Standard Curve (for reference only) of cTnI elisa kit    cTnI elisa kit Typical Testing Data/Standard Curve (for reference only) image
TOP
NCBI/Uniprot data below describe general gene information for cTnI. It may not necessarily be applicable to this product.
 NCBI GI #    49456923
 NCBI GeneID    7137
 NCBI Accession #    CAG46782.1 [Other Products]
 UniProt Primary Accession #    Q6FGX2 [Other Products]
 UniProt Related Accession #    P19429 [Other Products]
 Molecular Weight    24,008 Da
TOP
 NCBI Official Full Name    TNNI3
 NCBI Official Synonym Full Names    troponin I type 3 (cardiac)
 NCBI Official Symbol    TNNI3 [Similar Products]
 NCBI Official Synonym Symbols   

CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF

[Similar Products]
 NCBI Protein Information    troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive)
 UniProt Protein Name    TNNI3 protein
 UniProt Synonym Protein Names   

TNNI3 protein; Troponin I type 3 (Cardiac); Troponin I, cardiac; Troponin I, cardiac variant

 UniProt Gene Name    TNNI3 [Similar Products]
 UniProt Entry Name    Q6FGX2_HUMAN
 NCBI Summary for cTnI    Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
 UniProt Comments for cTnI    TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis; Motor; Actin-binding

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: sarcomere; troponin complex; cytosol

Molecular Function: troponin T binding; protein domain specific binding; troponin C binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; calcium-dependent protein binding; protein kinase binding

Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; regulation of systemic arterial blood pressure by ischemic conditions; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; muscle filament sliding; cardiac muscle contraction

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a
TOP
 UniProt References for cTnI    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H., Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M., Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M. , Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F., Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T., Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F., Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E., Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y., Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E., Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y., Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W., Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J., Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J., Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W., Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I., Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U., Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A., Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Cited for: NUCLEOTIDE SEQUENCE. "Cloning of human full-length CDSs in BD Creator(TM) System Donor vector."Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. Cited for: NUCLEOTIDE SEQUENCE. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases. Cited for: NUCLEOTIDE SEQUENCE. Tissue: HeartImportedAutomatic assertion inferred from database entries:EMBL:BAD97171.1. Rieder M.J., Bertucci C., Stanaway I.B., Johnson E.J., Swanson J.E., Siegel D.L., da Ponte S.H., Igartua C., Patterson K., Nickerson D.A. Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases. Cited for: NUCLEOTIDE SEQUENCE.
 Research Articles on cTnI    1. Cardiac TnI release is common before Norwood and BDG operations; then during the treatment protocol for hypoplastic left heart syndrome, cTnI release resolves.
TOP
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
TOP
Products similar to Human Cardiac Troponin I ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS2607757  Canine cardiac troponin I (cTn-I) ELISA Kit  MBS286037  Pig CardiactroponinI (cTn-I) ELISA Kit
 MBS9369616  Porcine High Sensitive Cardiac Troponin (HSCTN) ELISA Kit  MBS9353191  Bovine High Sensitive Cardiac Troponin (HSCTN) ELISA Kit
 MBS9352492  Rabbit High Sensitive Cardiac Troponin (HSCTN) ELISA Kit  MBS9346230  Fish High Sensitive Cardiac Troponin (HSCTN) ELISA Kit
 MBS9356235  Canine High Sensitive Cardiac Troponin (HSCTN) ELISA Kit  MBS9344256  Pigeon High Sensitive Cardiac Troponin (HSCTN) ELISA Kit
 MBS9364960  Chicken High Sensitive Cardiac Troponin (HSCTN) ELISA Kit  MBS9367326  Goose High Sensitive Cardiac Troponin (HSCTN) ELISA Kit
Pathways associated with cTnI elisa kitDiseases associated with cTnI elisa kit
 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >71 publications with cTnI and Heart Diseases
 Cardiomyopathies Antibodies  >63 publications with cTnI and Cardiomyopathies
 Cardiomyopathy, Hypertrophic, Familial Antibodies  >25 publications with cTnI and Cardiomyopathy, Hypertrophic, Familial
 Cardiomyopathy, Dilated Antibodies  >19 publications with cTnI and Cardiomyopathy, Dilated
 CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 7 Antibodies  >9 publications with cTnI and CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 7
 Cardiomyopathy, Restrictive Antibodies  >8 publications with cTnI and Cardiomyopathy, Restrictive
 Nervous System Diseases Antibodies  >5 publications with cTnI and Nervous System Diseases
 Hypertension Antibodies  >4 publications with cTnI and Hypertension
 Cardiomyopathy, Familial Restrictive, 1 Antibodies  >3 publications with cTnI and Cardiomyopathy, Familial Restrictive, 1
 Disease Models, Animal Antibodies  >2 publications with cTnI and Disease Models, Animal
Organs/Tissues associated with cTnI elisa kit
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >60 publications with cTnI and Heart
 Embryonic Tissue Antibodies  >2 publications with cTnI and Embryonic Tissue
 Lung Antibodies  >2 publications with cTnI and Lung
 Skin Antibodies  >1 publications with cTnI and Skin
 Uterus Antibodies  >1 publications with cTnI and Uterus
TOP
www.mybiosource.com/prods/ELISA-Kit
    Follow us on Facebook  Follow us onTwitter  Follow us on Google Plus  Subscribe to our RSS Feed for latest products and special promotions