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BH4 elisa kit :: Tetrahydrobiopterin (BH4) ELISA Kit

Catalog #    MBS733839 BH4 elisa kit
Unit / Price
48 Strip Wells  /  $470 +1 FREE 8GB USB
96 Strip Wells  /  $675 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

Tetrahydrobiopterin (BH4), ELISA Kit

 Also Known As   

Human tetrahydrobiopterin (BH4) ELISA Kit

 Product Gene Name   

BH4 elisa kit

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 BH4 elisa kit Manual   
 Specificity    This assay has high sensitivity and excellent specificity for detection of BH4. No significant cross-reactivity or interference between BH4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BH4 and all the analogues, therefore, cross reaction may still exist in some cases.
 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Intended Uses    This BH4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BH4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
 Sensitivity    The sensitivity in this assay is 0.1 ng/mL.
 Preparation and Storage    Store all reagents at 2-8 degree C
 Other Notes for BH4 elisa kit    Small volumes of BH4 elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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Product Description specifically for BH4 elisa kit

   Principle of the assay: BH4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BH4 antibody and an BH4-HRP conjugate. The assay sample and buffer are incubated together with BH4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BH4 concentration since BH4 from samples and BH4-HRP conjugate compete for the anti-BH4 antibody binding site. Since the number of sites is limited, as more sites are occupied by BH4 from the sample, fewer sites are left to bind BH4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BH4 concentration in each sample is interpolated from this standard curve.
 Searchable Name for BH4    anti-BH4; Buy BH4 elisa kit, Tetrahydrobiopterin (BH4) ELISA Kit (MBS733839) product datasheet at MyBioSource, ELISA Kits; BH4 elisa kit; anti-BH4
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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