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ELA1 elisa kit :: Human elastase 1, pancreatic ELISA Kit

Catalog #    MBS927335 ELA1 elisa kit
Unit / Price
48 Strip Wells  /  $540 +1 FREE 8GB USB
96 Strip Wells  /  $775 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

elastase 1, pancreatic (ELA1), ELISA Kit

 Also Known As   

Human Chymotrypsin-like elastase family member 1, ELA1 ELISA Kit

 Product Synonym Names    Human Chymotrypsin-like elastase family member 1 (ELA1) ELISA kit; ELA1; elastase 1; pancreatic; elastase 1; pancreatic
 Product Gene Name   

ELA1 elisa kit

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 ELA1 elisa kit Manual   
 Sequence Length    258
 OMIM    130120
 3D Structure    ModBase 3D Structure for Q9UNI1
 Species Reactivity    Human
 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ELA1. No significant cross-reactivity or interference between Human ELA1 and analogues was observed.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Sample Type    serum, plasma, tissue homogenates, cell culture supernates and cell lysates
 Detection Range    0.625 ng/ml-40 ng/ml
 Detection Wavelength    450 nm
 Sensitivity    0.156 ng/ml
 Sample Volume    50-100ul
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Other Notes for ELA1 elisa kit    Small volumes of ELA1 elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
 Searchable Terms for ELA1    anti-ELA1; Buy ELA1 elisa kit, Human elastase 1, pancreatic ELISA Kit (MBS927335) product datasheet at MyBioSource, ELISA Kits; 1990; CELA1_HUMAN; ELA1 elisa kit; anti-ELA1.
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Product Description specifically for ELA1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ELA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ELA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for ELA1. It may not necessarily be applicable to this product.
 NCBI GI #    58331209 [Other Products]
 NCBI GeneID    1990 [Other Products]
 NCBI Accession #    NP_001962.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_001971.5 [Other Products]
 UniProt Primary Accession #    Q9UNI1 [Other Products]
 UniProt Secondary Accession #    Q5MLF0; Q6DJT0; Q6ISM6 [Other Products]
 UniProt Related Accession #    Q9UNI1 [Other Products]
 Molecular Weight    27,798 Da
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 NCBI Official Full Name    chymotrypsin-like elastase family member 1
 NCBI Official Synonym Full Names    chymotrypsin-like elastase family, member 1
 NCBI Official Symbol    CELA1 [Similar Products]
 NCBI Official Synonym Symbols   

ELA1

[Similar Products]
 NCBI Protein Information    chymotrypsin-like elastase family member 1; elastase-1; pancreatic elastase 1; elastase 1, pancreatic
 UniProt Protein Name    Chymotrypsin-like elastase family member 1
 UniProt Synonym Protein Names   

Elastase-1; Pancreatic elastase 1

 UniProt Gene Name    CELA1 [Similar Products]
 UniProt Synonym Gene Names    ELA1 [Similar Products]
 UniProt Entry Name    CELA1_HUMAN
 NCBI Summary for ELA1    Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq, May 2009]
 UniProt Comments for ELA1    Function: Acts upon elastin.

Catalytic activity: Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.

Cofactor: Binds 1 calcium ion per subunit
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 UniProt References for ELA1    1. "Human elastase 1: evidence for expression in the skin and the identification of a frequent frameshift polymorphism."Talas U., Dunlop J., Khalaf S., Leigh I.M., Kelsell D.P.J. Invest. Dermatol. 114:165-170(2000) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Keratinocyte.
2. "The finished DNA sequence of human chromosome 12."Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. NIEHS SNPs program Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-194.
4. "The consensus coding sequences of human breast and colorectal cancers."Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]. Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-76.
 Research Articles on ELA1    1. Fecal elastase-1 is useful to identify pancreatic insufficiency. Steatorrhea is anticipated in non-operated patients only when FE-1 is below the limit for a confident measurement of our assay
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 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Diseases associated with ELA1 elisa kit
 Disease Name  Pubmed Publications
 Endocrine System Diseases Antibodies  >1 publications with ELA1 and Endocrine System Diseases
 Neoplasms Antibodies  >1 publications with ELA1 and Neoplasms
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