Antibody Protein ELISA Kit from MyBioSource
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ATG12 elisa kit :: Human ATG12 autophagy related 12 homolog (S. cerevisiae) ELISA Kit

Catalog #    MBS944212 ATG12 elisa kit
Unit / Price
48 Strip Wells  /  $540 +1 FREE 8GB USB
96 Strip Wells  /  $775 +1 FREE 8GB USB
 
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name   

ATG12 autophagy related 12 homolog (S. cerevisiae) , ELISA Kit

 Also Known As   

Human Ubiquitin-like protein ATG12, ATG12 ELISA Kit

 Product Synonym Names    Human Ubiquitin-like protein ATG12 (ATG12) ELISA kit; APG12; APG12L; FBR93; HAPG12; APG12 autophagy 12-like; Apg12 (autophagy; yeast) homolog; ATG12 autophagy related 12 homolog (S. cerevisiae)
 Product Gene Name   

ATG12 elisa kit

[Similar Products]
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 ATG12 elisa kit Manual   
 Immunogen Sequence Length    140
 OMIM    609608
 3D Structure    ModBase 3D Structure for O94817
 Species Reactivity    Human
 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ATG12. No significant cross-reactivity or interference between Human ATG12 and analogues was observed.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Sample Type    serum, plasma, tissue homogenates, cell lysates
 Detection Range    25 pg/ml-1600 pg/ml
 Detection Wavelength    450 nm
 Sensitivity    6.25 pg/ml
 Sample Volume    50-100ul
 Protein Biological Process 1    Apoptosis/Autophagy
 Protein Biological Process 3    Autophagy
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for ATG12 elisa kit    Small volumes of ATG12 elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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NCBI/Uniprot data below describe general gene information for ATG12. It may not necessarily be applicable to this product.
 NCBI GI #    480540330 [Other Products]
 NCBI GeneID    9140 [Other Products]
 NCBI Accession #    NP_001264712.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001277783.1 [Other Products]
 UniProt Primary Accession #    O94817 [Other Products]
 UniProt Secondary Accession #    Q6PJV2 [Other Products]
 UniProt Related Accession #    O94817 [Other Products]
 Molecular Weight    15,113 Da
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 NCBI Official Full Name    ubiquitin-like protein ATG12 isoform 2
 NCBI Official Synonym Full Names    autophagy related 12
 NCBI Official Symbol    ATG12 [Similar Products]
 NCBI Official Synonym Symbols   

APG12; FBR93; APG12L; HAPG12

[Similar Products]
 NCBI Protein Information    ubiquitin-like protein ATG12; Apg12 (autophagy, yeast) homolog; ATG12 autophagy related 12 homolog
 UniProt Protein Name    Ubiquitin-like protein ATG12
 UniProt Synonym Protein Names   

Autophagy-related protein 12; APG12-like

 UniProt Gene Name    ATG12 [Similar Products]
 UniProt Synonym Gene Names    APG12; APG12L; APG12-like [Similar Products]
 UniProt Entry Name    ATG12_HUMAN
 NCBI Summary for ATG12    Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
 UniProt Comments for ATG12    Function: Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.7 PublicationsManual assertion based on experiment in:Ref.5
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 UniProt References for ATG12    1. "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy."Mizushima N., Sugita H., Yoshimori T., Ohsumi Y.J. Biol. Chem. 273:33889-33892(1998) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CONJUGATION TO ATG5, TISSUE SPECIFICITY.
2. "The DNA sequence and comparative analysis of human chromosome 5."Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Ovary.
4. "The human homolog of Saccharomyces cerevisiae Apg7p is a Protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3."Tanida I., Tanida-Miyake E., Ueno T., Kominami E.J. Biol. Chem. 276:1701-1706(2001) [PubMed] [Europe PMC] [Abstract]. Cited for: INTERACTION WITH ATG7, CONJUGATION TO ATG5.
5. "Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing."Tanida I., Nishitani T., Nemoto T., Ueno T., Kominami E.Biochem. Biophys. Res. Commun. 296:1164-1170(2002) [PubMed] [Europe PMC] [Abstract]. Cited for: INTERACTION WITH ATG3, CONJUGATION TO ATG5, FUNCTION.
 Research Articles on ATG12    1. Cav-1 competitively interacts with the ATG12-ATG5 system to suppress the formation and function of the latter in lung epithelial cells.
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 Searchable Name for ATG12    anti-ATG12; Buy ATG12 elisa kit ($540), Human ATG12 autophagy related 12 homolog (S. cerevisiae) ELISA Kit (MBS944212) product datasheet at MyBioSource, ELISA Kits; 9140; ATG12_HUMAN; ATG12 elisa kit; anti-ATG12
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Products associated with ATG12 elisa kitPathways associated with ATG12 elisa kit
 Reference Product  PubMed Publications
 ATG5 elisa kit  >108 publications with ATG12 and ATG5
 ATG7 elisa kit  >40 publications with ATG12 and ATG7
 ATG3 elisa kit  >19 publications with ATG12 and ATG3
 ATG10 elisa kit  >19 publications with ATG12 and ATG10
 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies  Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways antibodies  RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
Diseases associated with ATG12 elisa kitOrgans/Tissues associated with ATG12 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >20 publications with ATG12 and Nervous System Diseases
 Neoplasms Antibodies  >19 publications with ATG12 and Neoplasms
 Necrosis Antibodies  >7 publications with ATG12 and Necrosis
 Drug Toxicity Antibodies  >5 publications with ATG12 and Drug Toxicity
 Cardiovascular Diseases Antibodies  >4 publications with ATG12 and Cardiovascular Diseases
 Heart Diseases Antibodies  >2 publications with ATG12 and Heart Diseases
 Muscular Diseases Antibodies  >2 publications with ATG12 and Muscular Diseases
 Lung Neoplasms Antibodies  >2 publications with ATG12 and Lung Neoplasms
 Cardiomyopathies Antibodies  >2 publications with ATG12 and Cardiomyopathies
 Ataxia Antibodies  >2 publications with ATG12 and Ataxia
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13 publications with ATG12 and Brain
 Muscle Antibodies  >12 publications with ATG12 and Muscle
 Liver Antibodies  >7 publications with ATG12 and Liver
 Nerve Antibodies  >7 publications with ATG12 and Nerve
 Blood Antibodies  >5 publications with ATG12 and Blood
 Heart Antibodies  >4 publications with ATG12 and Heart
 Stomach Antibodies  >4 publications with ATG12 and Stomach
 Lung Antibodies  >4 publications with ATG12 and Lung
 Prostate Antibodies  >2 publications with ATG12 and Prostate
 Vascular Antibodies  >2 publications with ATG12 and Vascular
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