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ATG12 elisa kit :: Ubiquitin-like protein ATG12 (ATG12) [ELISA Kit]
Catalog #    MBS944212
Unit / Price
48 Strip Wells  /  $585.00 + 1 FREE 8GB USB Flash Drive
96 Strip Wells  /  $775.00 + 1 FREE 8GB USB Flash Drive
    
 Go to:     Product Names    Product Info    Accession #s    Product Desc    Diseases/Tissues/Pathways    Applications    References 
 Product Name    Ubiquitin-like protein ATG12 (ATG12), ELISA Kit
 Also Known As    Human Ubiquitin-like protein ATG12 (ATG12) ELISA Kit
 Product Synonym Names    ATG12 autophagy related 12 homolog (S. cerevisiae); APG12; APG12L; FBR93; HAPG12; APG12 autophagy 12-like|Apg12 (autophagy; yeast) homolog
 Product Gene Name    ATG12 elisa kit [Similar Products]
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 Request for Manual   
 Immunogen Sequence Length    140
 Chromosome Location    Chromosome: 5; NC_000005.9 (115163893..115177548, complement). Location: 5q21-q22
 OMIM    609608
 3D Structure    ModBase 3D Structure for O94817
 Species Reactivity    Human
 Principle    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATG12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATG12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATG12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATG12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
 Sample Type    Serum, plasma
 Sample Volume    50-100ul
 Detection Wavelength    450 nm
 Assay Time    1-5h
 Protein Biological Process    Protein Biological Process: Apoptosis/Autophagy
Protein Biological Process 3: Autophagy
 Precision    Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 Sample Collection    Please refer to the manual for the last samples information.
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 degree C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 degree C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 degree C or -80 degree C. Avoid repeated freeze-thaw cycles.
 Technical Tips    1. Storage: Please store unopened kit at 2 to 8 degree C and use the kit before the expiration date. For opened kit, which is vulnerable to germ and contamination, please use it as soon as possible. NEVER store the ELISA plate at -20 degree C and the substrate solution should be kept away from light both during storage and usage.
2. Reagents preparation: Please prepare reagents 10 minutes before usage. Centrifugation is required for all reagent components when using for the first time so as to concentrate them to the bottom of the tube. Please use precise measuring tools (pipette, graduated cylinder etc...) for reagent preparation due the actual quantity provided for each reagent (eg: biotin-antibody, HRP avidin, Sample Diluent) is more than what the label indicates. NEVER take the labeled quantity for granted and add solution directly into the vials for reagent preparation. All experimental equipments should be clean and please refer to the insert for details of reagent preparation.
3. ELISA plate: It's OK that the aluminum foil bags is leaking since it won't affect the quality of the plate. The strips are removable and it's recommended to separate those wells needed for assay and store the rest at 2-8 degree C in the dark without germ contamination. NEVER expose the whole plate when only some of them are needed.
4. Samples or reagents adding: The whole time for sample adding should be controlled in 3 minutes
and added volume for each well should be the same to have reaction consistency.
5. Incubation: To avoid sample evaporation and contamination, proper adhesion of plate sealers during incubation steps is necessary. Be careful and do not spill the liquid when moving the plate. Since temperature stability is very important, please carefully monitor incubating temperature and keep it at 37 degree C. Avoid opening the door of the incubator multiple times during incubation.
6. Washing: Please ensure the same sample dilution volume per well and you should use multichannel pipette. If you use the ELISA Washer, please make sure it's clean and without contamination. Don't rinse but gently wash. Throw plates vertically and wash it thoroughly to reduce the edge effect. For detailed procedure, please refer to the manual insert.
7. Reading: Please read the plate in 5 minutes after adding the stop solution to avoid generation of brown precipitate that may affect the result. Make sure that the microplate reader is set correctly and the filter is precisely calibrated.
8. Do not make mix use of reagents within different lot #. Also, cross-use of caps matched to different reagents is not recommended. NEVER replace components of our kit with others.
 Other Notes for ATG12 elisa kit    Small volumes of ATG12 elisa kit may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
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NCBI/Uniprot data below describe general gene information for ATG12. It may not necessarily be applicable to this product.
 NCBI GI #    290560746 [Other Products]
 NCBI GeneID    9140 [Other Products]
 NCBI Accession #    NP_004698.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_004707.3 [Other Products]
 UniProt Primary Accession #    O94817 [Other Products]
 UniProt Secondary Accession #    Q6PJV2 [Other Products]
 UniProt Related Accession #    O94817 [Other Products]
 Molecular Weight    15,113 Da
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 NCBI Official Full Name    ubiquitin-like protein ATG12 isoform 1
 NCBI Official Synonym Full Names    autophagy related 12
 NCBI Official Symbol    ATG12 [Similar Products]
 NCBI Official Synonym Symbols    APG12; FBR93; APG12L; HAPG12 [Similar Products]
 NCBI Protein Information    ubiquitin-like protein ATG12; Apg12 (autophagy, yeast) homolog; ATG12 autophagy related 12 homolog
 UniProt Protein Name    Ubiquitin-like protein ATG12
 UniProt Synonym Protein Names    Autophagy-related protein 12
 UniProt Gene Name    ATG12 [Similar Products]
 UniProt Synonym Gene Names    APG12; APG12L; APG12-like [Similar Products]
 UniProt Entry Name    ATG12_HUMAN
 NCBI Summary for ATG12    Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
 UniProt Comments for ATG12    Function: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.

Subcellular location: Cytoplasm

By similarity.

Tissue specificity: Ubiquitous. Ref.1

Domain: Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage. Ref.4

Sequence similarities: Belongs to the ATG12 family.

Sequence caution: The sequence AAH12266.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
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 NCBI References for ATG12    1. Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy.
2. A mammalian autophagosome maturation mechanism mediated by TECPR1 and the Atg12-Atg5 conjugate.
3. Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.
4. Autophagy proteins LC3B, ATG5 and ATG12 participate in quality control after mitochondrial damage and influence lifespan.
5. The autophagy protein Atg12 associates with antiapoptotic Bcl-2 family members to promote mitochondrial apoptosis.
 UniProt References for ATG12    • A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. Mizushima N., Sugita H., Yoshimori T., Ohsumi Y.J. Biol. Chem. 273:33889-33892(1998) [PubMed] [Europe PMC] [Abstract]
 Research Articles on ATG12    1. study to identify role of conjugation between ATG12 and ATG5 in LC3 lipidation; structural and mutational analyses of ATG12~ATG5-ATG16N revealed the conjugation generates a patch across ATG12 and ATG5 required for E3 activity
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 Searchable Name for ATG12    anti-ATG12; Buy ATG12 elisa kit :: Ubiquitin-like protein ATG12 (ATG12), ELISA Kit (MBS944212) datasheet at MyBioSource; anti-ATG12; APG12; FBR93; APG12L; HAPG12; APG12; APG12L; APG12-like; anti-Ubiquitin-like protein ATG12 (ATG12); Human Ubiquitin-like protein ATG12 (ATG12) ELISA Kit; ATG12 autophagy related 12 homolog (S. cerevisiae); APG12; APG12L; FBR93; HAPG12; APG12 autophagy 12-like|Apg12 (autophagy; yeast) homolog; ubiquitin-like protein ATG12 isoform 1; autophagy related 12; Ubiquitin-like protein ATG12; Autophagy-related protein 12 anti-Human. (NCBI/UniProt 290560746 NP_004698.3 O94817 NM_004707.3 O94817 Q6PJV2 15,113 Da); Chromosome: 5; NC_000005.9 (115163893..115177548, complement). Location: 5q21-q22; 9140; ATG12_HUMAN; ATG12 elisa kit; anti-ATG12
 Precautions    This product is for research use only. Not for use in diagnostic or therapeutic procedures.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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Diseases associated with ATG12 elisa kitOrgans/Tissues associated with ATG12 elisa kit
 Disease Name  Ref Prod  Pubmed Publications
 Nervous System Diseases Antibodies  anti-ATG12  >20 publications with ATG12 and Nervous System Diseases
 Neoplasms Antibodies  anti-ATG12  >19 publications with ATG12 and Neoplasms
 Necrosis Antibodies  anti-ATG12  >7 publications with ATG12 and Necrosis
 Drug Toxicity Antibodies  anti-ATG12  >5 publications with ATG12 and Drug Toxicity
 Cardiovascular Diseases Antibodies  anti-ATG12  >4 publications with ATG12 and Cardiovascular Diseases
 Heart Diseases Antibodies  anti-ATG12  >2 publications with ATG12 and Heart Diseases
 Muscular Diseases Antibodies  anti-ATG12  >2 publications with ATG12 and Muscular Diseases
 Lung Neoplasms Antibodies  anti-ATG12  >2 publications with ATG12 and Lung Neoplasms
 Cardiomyopathies Antibodies  anti-ATG12  >2 publications with ATG12 and Cardiomyopathies
 Ataxia Antibodies  anti-ATG12  >2 publications with ATG12 and Ataxia
 Organ/Tissue Name  Ref Prod  Pubmed Publications
 Brain Antibodies  ATG12 antibody  >13 publications with ATG12 and Brain
 Muscle Antibodies  ATG12 antibody  >12 publications with ATG12 and Muscle
 Liver Antibodies  ATG12 antibody  >7 publications with ATG12 and Liver
 Nerve Antibodies  ATG12 antibody  >7 publications with ATG12 and Nerve
 Blood Antibodies  ATG12 antibody  >5 publications with ATG12 and Blood
 Heart Antibodies  ATG12 antibody  >4 publications with ATG12 and Heart
 Stomach Antibodies  ATG12 antibody  >4 publications with ATG12 and Stomach
 Lung Antibodies  ATG12 antibody  >4 publications with ATG12 and Lung
 Prostate Antibodies  ATG12 antibody  >2 publications with ATG12 and Prostate
 Vascular Antibodies  ATG12 antibody  >2 publications with ATG12 and Vascular
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