View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List anti-BrdU antibody :: Rat BrdU Monoclonal Antibody Catalog # MBS215474 Unit / Price DISCONTINUEDYou will be redirected to our replacement product Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name BrdU, Monoclonal Antibody Full Product Name RAT ANTI BrdU:FITC Product Gene Name anti-BrdU antibody[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG2a Clone Number BU1/75 (ICR1) Host Rat Form/Format FITCPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid Concentration IgG concentration 0.5 mg/ml (lot specific) Perservative Stabilisers 0.09% Sodium Azide (NaN3)50% GlycerolPreparation Buffer Solution Phosphate buffered saline Target Species Chemical Preparation and Storage Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Shelf Life: 18 months from date of despatch. Other Notes Small volumes of anti-BrdU antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-BrdU antibody Rat anti BrdU antibody clone BU1/75 (ICR1), recognizes bromodeoxyuridine (known as BrdU or BrdUrd). Rat anti BrdU antibody clone BU1/75 (ICR1) reacts with BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Rat anti BrdU antibody, clone BU1/75 (ICR1) cross reacts with chlorodeoxyuridine (CldU) but does not cross react with thymidine or iododeoxyuridine (Aten et al. 1992). BrdU, IdU and CldU are analogs of thymidine, they can incorporate into DNA during DNA synthesis replacing thymidine.Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004) Clone BU1/75 (ICR1) has been used to detect CldU to study the speed of DNA replication fork (Bugler et al. 2010), in the detection of CldU label retaining stem cells (Kimoto et al. 2008) and label retaining neurons (Murata et al. 2011). Applications Tested/Suitable for anti-BrdU antibody Flow cytometry (FC/FACS)* Application Notes for anti-BrdU antibody Flow Cytometry: Use 20ul of the suggested working dilution to label 106 cells in 100ul.Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/20; Application Note: See recommended protocol below. Testing Data of anti-BrdU antibody Formalin fixed, paraffin embedded mouse colon stained with Rat anti BrdU (MBS212468): med power Testing Data of anti-BrdU antibody Formalin fixed, paraffin embedded mouse colon stained with Rat anti BrdU (MBS212468): high power Testing Data of anti-BrdU antibody Published customer image: Impaired base-line proliferation in the hippocampus in the absence of abeta T cells. (A) Representative BrdU immunohistochemistry of the hippocampal dentate gyrus from eight week-old wild-type (A1), TCRa +/- (A2) and TCRa -/- (A3) mice, 24 hours after the first of 3 consecutive BrdU injections. Scale bar, 100 um. (B) Quantification of BrdU + cells in the dentate gyrus of wild-type (n = 6), TCRa +/- (n = 7) and TCRa -/- (n = 8) mice. All numbers are mean +/- SEM. ANOVA, * p < 0.05.From: Niebling J, E. Rnker A, Schallenberg S et al. Myelin-specific T helper 17 cells promote adult hippocampal neurogenesis through indirect mechanisms [v1; ref status: indexed, http://f1000r.es/3ny] F1000Research 2014, 3:169. Testing Data of anti-BrdU antibody Formalin fixed, paraffin embedded mouse colon stained with Rat anti BrdU (MBS212468): low power Testing Data of anti-BrdU antibody HL-60 cells were pulse labeled with BrdU for 45 minutes prior to harvesting and then incubated with primary antibody Rat anti BrdU (MBS219768) clone BU1/75 diluted 1/100 followed by Rabbit anti Rat IgG secondary antibody (MBS235146) FITC-conjugated, diluted 1/200 TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP