View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List anti-CA-125 antibody :: Mouse anti-Human CA-125 Monoclonal Antibody Catalog # MBS370011 Unit / Price 5 Slides / $200 +1 FREE 8GB USB 0.1 mL (Concentrate) / $255 +1 FREE 8GB USB 3 mL (RTU) / $290 +1 FREE 8GB USB 7 mL (RTU) / $445 +1 FREE 8GB USB 0.5 mL (Concentrate) / $640 +1 FREE 8GB USB 15 mL (RTU) / $730 +1 FREE 8GB USB 1 mL (Concentrate) / $1,010 +1 FREE 8GB USB 5x15 mL (RTU) / $3,120 +3 FREE 8GB USB 5x1 mL (Concentrate) / $4,380 +5 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name CA-125, Monoclonal Antibody Popular Item Full Product Name CA-125 Product Gene Name anti-CA-125 antibody[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG1/K Clone Number OC125 Host Mouse Species Reactivity Human Form/Format Anti-CA-125 is a Mouse Monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5,containing BSA and sodium azide as a preservative. Immunogen Partially purified human mucin fraction from a pool of tissues from patients with epithelial ovarian cancer. Localization Cytoplasmic, Membranous Control Colon Pancreas, Epithelioid Mesothelioma, Ovarian Carcinoma Precautions 1.This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.2. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.3. Dispose of unused solution with copious amount of water.4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).7. For additional safety information refer to Safety Data Sheet for this product.8. For complete recommendations for handling biological specimens, please refer to the CDC document, Guidelines for Safe Work Practices in Human and AMDL. Mounting Protocols For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter. Specimen Preparation Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. Tissue should remain hydrated via use of Immuno/DNA Washer solutions.Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations. Product Limitations Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized, and incubation time), optimal performance should be established through the use of positive and negative controls. Preparation and Storage Store at 2-8°C (Control Slides: Store at 20-25°C)This product is stable up to the expiration date on the product label. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions. ISO Certification Manufactured in an ISO 13485:2016 Certified Laboratory. Other Notes Small volumes of anti-CA-125 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-CA-125 antibody CA-125 reacts with malignant ovarian epithelial cells. CA-125 also reacts with antigens in Seminal Vesicle Carcinoma and Anaplastic Lymphoma. In adult tissues, CA-125 is found in epithelial cells of Fallopian tube, endometrium and endocervix, pancreas, colon, gall bladder, stomach, kidney, apocrine sweat gland, and mammary gland. It is also found in mesothe-lial cell lining of pleura, pericardium and peritoneum. It is found in ovarian tumors of serous, endometrioid or clear-cell types and Adenocarcinomas of Mullerian type. Applications Tested/Suitable for anti-CA-125 antibody Paraffin , Frozen Application Notes for anti-CA-125 antibody Specimen Preparation:Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. Tissue should remain hydrated via use of Immuno/DNA Washer solutions. Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.Staining Procedure:1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positively charged slides such as Hydrophilic Plus Slides.2. Air dry for 2 hours at 58° C.3. Deparaffinize, dehydrate and rehydrate tissues.,4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.5. Any of three heating methods may be used:a. TintoRetriever Pressure Cooker or EquivalentPlace tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.b. TintoRetriever PT Module or Water Bath MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.c. Conventional Steamer MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes.6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturers instructions.8. Wash slides with ImmunoDNA washer or DI water.9. Continue IHC staining protocol. Wash slides between each step with ImmunoDNA washer solution. Dilution Information of anti-CA-125 antibody Testing Data of anti-CA-125 antibody Abbreviated Immunohistochemical Protocol Immunohistochemistry (IHC) of anti-CA-125 antibody TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP