View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Mouse V5 epitope tag Monoclonal Antibody Catalog # MBS601200 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name V5 epitope tag, Monoclonal Antibody Full Product Name V5 epitope tag (Pk-tag, paramyxovirus, SV5) Product Synonym Names Anti -V5 epitope tag (Pk-tag, paramyxovirus, SV5) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Monoclonal Isotype IgG2a Clone Number 9A208 Host Mouse Specificity Recognizes recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. (V5-Tag). Detects recombinant proteins including transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. Purity/Purification Affinity PurifiedPurified by Protein G affinity chromatography. Form/Format Supplied as a liquid in 0.09% sodium azide. Immunogen Paramyxovirus SV5, Simian-Virus 5. Hybridoma Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line. Preparation and Storage May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Other Notes Small volumes of anti-V5 epitope tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Product Categories/Family for anti-V5 epitope tag antibody Antibodies; Abs to Assay Detection Applications Tested/Suitable for anti-V5 epitope tag antibody ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA) Application Notes for anti-V5 epitope tag antibody Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot, and RIA.Other applications have not been tested.Dilution: ELISA: 1:1000-1:5000Western Blot: 1:1000-1:5000Immunohistochemistry: frozen TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP