View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Mouse alpha-Gal Epitope Monoclonal Antibody Catalog # MBS603355 Unit / Price 1 Each / $920 +1 FREE 8GB USB 5x1 Each / $3,975 +4 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name alpha-Gal Epitope, Monoclonal Antibody Popular Item Full Product Name alpha-Gal Epitope (Galalpha1-3Galbeta1-4GlcNAc-R) Product Synonym Names Anti -alpha-Gal Epitope (Gal 1-3Gal 1-4GlcNAc-R) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP MBS603355 COA COA PDF Clonality Monoclonal Isotype IgM Clone Number 6D407 Host Mouse Specificity Recognizes synthetic and naturally produced rabbit Gal alpha 1-3 Gal epitopes on glycoproteins and glycolipids. Purity/Purification Supernatant Form/Format Supplied as a liquid. Immunogen Rabbit red blood cell membrane Cross Reactivity Mouse, rat and porcine.Does not crossreact with beta-Gal glycoproteins or BSA. Preparation and Storage May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Other Notes Small volumes of anti-alpha-Gal Epitope antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-alpha-Gal Epitope antibody Widely described antibody against alpha-galactose residues with broad cross-reactivity among different species. Useful for measuring the alpha-Gal epitope expression on cells, glycolipids and glycoproteins, characterization of hyperacute rejection (HAR) in organ and tissue transplantations and monitoring xenotransplantation experiments. The IgM isotype mimics in vivo reactions and can be used for cytotoxicity assays for alpha -Gal specific pathways (addition or presence of complement necessary). Ideal as a high-throughput screening tool for inhibitors of antibody induced cytotoxicity. Organ transplantation from pig to human result in HAR. Humans naturally produce large quantities of anti-alpha-Gal antibodies, which represent 1-3% of all circulating immunoglobulins and are produced by about 1% of all B cells. When pig organs or tissues are transplanted into the human body, the IgM isotype of anti-Gal binds to alpha-Gal epitopes, which causes activation of the complement cascade, resulting in cell lysis. This rapid activation of complement by anti-Gal IgM is an immunological barrier that poses the greatest risk of initiating HAR. Product Categories/Family for anti-alpha-Gal Epitope antibody Antibodies; Abs to Enzymes Applications Tested/Suitable for anti-alpha-Gal Epitope antibody ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Functional Studies (can be used for cytotoxicity assays for alpha-Gal specific pathways)Other applications not tested Application Notes for anti-alpha-Gal Epitope antibody ELISA: 1:5Western Blot: 1:5Flow Cytometry: 1:5Immunohistochemistry (Paraffin): 1:5Note: Do not use non-fat milk for blocking. Many blocking agents from non-human mammal sources will crossreact with the antibody.Optimal dilutions to be determined by the researcher. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP