View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Rabbit Cre Recombinase Polyclonal Antibody Catalog # MBS613458 Unit / Price 0.05 mL / $735 +1 FREE 8GB USB 5x0.05 mL / $3,125 +3 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Cre Recombinase, Polyclonal Antibody Full Product Name Cre Recombinase (Cre, Cre (Cyclization Recombination) Enzyme) Product Synonym Names Anti -Cre Recombinase (Cre, Cre (Cyclization Recombination) Enzyme) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Isotype IgG Host Rabbit Specificity Recognizes Cre/lox systems at ~36-38kD. Purity/Purification Affinity PurifiedPurified by peptide affinity chromatography. Form/Format Supplied as a liquid in PBS, 0.1% sodium azide, 30% glycerol. Immunogen Synthethic peptide corresponding to aa57-73, KLNNRKWFPAEPEDVRD of the bacteriophage protein CRE (GenBank# AAQ14086.1). Positive Control Recombinant CRE, Cells stably transfected with CRE, Tissue from a CRE transgenic organism Preparation and Storage May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Other Notes Small volumes of anti-Cre Recombinase antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-Cre Recombinase antibody The Cre enzyme, a member of a large family of recombinases, recognizes loxP which is a sequence motif of 34bp from the PI bacteriophage. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that segment leaving a single loxP site in the remaining DNA molecule. The excised DNA segment is subsequently degraded. Cre and lox comprise the Cre/lox system which was first developed in the late 1980's to artificially manipulate gene expression. As an example, mice with the Cre protein expressed in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites. When the mice are bred, the cells carrying Cre will cause those cells to lose the target gene. If the Cre gene is, for example, bound to a promoter that only allows Cre production in neuronal cells, the target gene will be deleted only in those cells. There are numerous animal, plant and bacterial stocks that already contain the Cre gene driven by ubiquitous, tissue-specific or inducible promoters and provide a quick method for breeding experiments. The Cre/lox system has the advantage of working in almost any type of cell. This has led to its application in many experiments, for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells. Product Categories/Family for anti-Cre Recombinase antibody Antibodies; Abs to Enzymes Applications Tested/Suitable for anti-Cre Recombinase antibody Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF) Application Notes for anti-Cre Recombinase antibody Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry.Dilution: Western Blot: 1:1000-1:4000Immunoprecipitation: 1:50-1:200Immunohistochemistry (Paraffin): 1:1000-1:5000. May also be used in frozen tissues at an assay-dependent dilution. Western Blot (WB) of anti-Cre Recombinase antibody Western Blot analysis of HeLa cells stably transfected with a Cre expression plasmid (lane 1) and Hela cells transfected with a neo-plasmid (negative control; lane 2) using MBS613458 (1:2000). Immunohistochemistry (IHC) of anti-Cre Recombinase antibody Immunohistochemical analysis of formalin-fixed, paraffin-embedded tissue section from cerebellum of a Cre transgenic mouse using MBS613458 (1:2000). The arrow points to a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain was used. Immunohistochemistry (IHC) of anti-Cre Recombinase antibody Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using MBS613458 (1:2000). In this mouse line, Cre is expressed in the neurons. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain. TOP NCBI/Uniprot data below describe general gene information for Cre Recombinase. It may not necessarily be applicable to this product. NCBI GI # 194473384 TOP NCBI Official Full Name cre recombinase TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. 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TOP Products associated with anti-Cre Recombinase antibody Reference Product PubMed Publications FLNA antibody >12 publications with Cre Recombinase and FLNA Pathways associated with anti-Cre Recombinase antibody Products by Pathway Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Organs/Tissues associated with anti-Cre Recombinase antibody Organ/Tissue Name Pubmed Publications Bone Antibodies >13 publications with Cre Recombinase and Bone Connective Tissue Antibodies >4 publications with Cre Recombinase and Connective Tissue Heart Antibodies >3 publications with Cre Recombinase and Heart Muscle Antibodies >3 publications with Cre Recombinase and Muscle Brain Antibodies >3 publications with Cre Recombinase and Brain Vascular Antibodies >2 publications with Cre Recombinase and Vascular Embryonic Tissue Antibodies >2 publications with Cre Recombinase and Embryonic Tissue Ear Antibodies >1 publications with Cre Recombinase and Ear Kidney Antibodies >1 publications with Cre Recombinase and Kidney Umbilical Cord Antibodies >1 publications with Cre Recombinase and Umbilical Cord TOP