View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List anti-HIS antibody :: Rabbit HIS tag Polyclonal Antibody Catalog # MBS440057 Unit / Price 0.2 mg / $300 +1 FREE 8GB USB 5x0.2 mg / $1,170 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name HIS tag, Polyclonal Antibody Full Product Name HIS tag (H20) Antibody Product Gene Name anti-HIS antibody[Similar Products] Antibody/Peptide Pairs HIS peptide (MBS443057) is used for blocking the activity of HIS antibody (MBS440057) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Rabbit Specificity Detects proteins with the polyhistidine Tag Form/Format 200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. Concentration 0.200 mg/ml (lot specific) Origin His (H20) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide containing the 6x histidine tag. Immunogen Synthetic a peptide containing the 6x histidine tag. Preparation and Storage Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment. Other Notes Small volumes of anti-HIS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-HIS antibody The 6x histidine recombinant protein tag is made up of 6 histidine residues attached to either the amino or carboxy terminus of the protein being expressed. The recombinant protein can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6x histidine in techniques such as western blotting and immunoprecipitation. Applications Tested/Suitable for anti-HIS antibody Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Application Notes for anti-HIS antibody Western blot analysis of polyhistidine tagged recombinant HspBP1 at 0.1 ug (A) and 0.01 ug (B). Western Blot (WB) of anti-HIS antibody TOP Product References and Citations for anti-HIS antibody 1.) Wu B, Wilmouth RC. 2008. Proteomics analysis of immunoprecipitated proteins associated with the oncogenic kinase cot. Mol Cells. Feb 29;25(1):43-9. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP