View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List CMRL1066 10X w/o L-Glutamine, Phosphate Culture Media Catalog # MBS652964 Unit / Price 100 mL / $225 +1 FREE 8GB USB 1 L / $345 +1 FREE 8GB USB 10 L / $1,485 +1 FREE 8GB USB 5x10 L / $6,425 +8 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name CMRL1066 10X w/o L-Glutamine, Phosphate, Culture Media Popular Item Full Product Name CMRL1066 10X w/o L-Glutamine, Phosphate (Powder) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Purity/Purification Cell Culture Grade Form/Format Light orange, homogeneous, free flowing powder Endotoxin Level <1EU/mL Directions per Liter of 10X Dissolve 96.12g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 22g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Some precipitate may be observed due to excess concentration of salts. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Note: Lab recommends the preparation of this product as a 1X solution. pH 4.4 Solubility(1X) Yellow, clear, complete Media Formulation Components shown as g/LiterInorganic Salts: 10XCalcium Chloride: 2.00Magnesium Sulfate, anhy: 0.9769Potassium Chloride: 4.00Sodium Acetate, anhy: 0.50Sodium Chloride: 68.0Sodium Phosphate Mono, anhy: AbsentAmino AcidsL-Alanine 0.25L-Arginine 0.5787L-Aspartic Acid 0.30L-Cysteine• HCL• H2O 2.60L-Cystine 0.20L-Glutamic Acid, Sodium 0.862L-Glutamine AbsentGlycine 0.50L-Histidine• HCl•H2O 0.20trans-4-Hydroxy-L-Proline 0.10L-Isoleucine 0.20L-Leucine 0.60L-Lysine• HCl 0.70L-Methionine 0.15L-Phenylalanine 0.25L-Proline 0.40L-Serine 0.25L-Threonine 0.30L-Tryptophan 0.10L-Tyrosine 0.40L-Valine 0.25VitaminsPABA 0.0005L-Ascorbic Acid• Na 0.50D-Biotin 0.0001Choline Chloride 0.005Coenzyme A• Na 0.025Cocarboxylase 0.012'-Deoxyadenosine 0.102'-Deoxycytidine• HCl 0.1162'-Deoxyguanosine 0.10Folic Acid 0.0001myo-Inositol 0.00055-Methyldeoxycytidine 0.001ß-NAD 0.070ß-NADP• Na 0.010Nicotinic Acid 0.00025Niacinamide 0.00025D-Pantothenic Acid• Ca 0.0001Pyridoxal• HCl 0.00025Pyridoxine• HCl 0.00025Riboflavin 0.0001Flavin Adenine Dinucleotide• 2Na 0.00106Thiamine• HCl 0.0001 Preparation and Storage Store powdered media at room tempeture. Open bottles shoould be capped tightly and kept in a dark low humidity environment. Prepared media should be ketp at 4°C. Other Notes Small volumes of CMRL1066 10X w/o L-Glutamine, Phosphate culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for CMRL1066 10X w/o L-Glutamine, Phosphate culture media CMRL-1066 is a chemically defined medium developed in the late 1960's at the Connaugh Medical Research Laboratories. CMRL-1066 was designed initially for use with mouse L-cells in non-supplemented culture. Although developed for use in serum-free culture, CMRL-1066 can be supplemented with serum and used to support the growth of many cell types. Suitable for growing a wide variety of cell types, especially fibroblast-like cells. Product Categories/Family for CMRL1066 10X w/o L-Glutamine, Phosphate culture media Culture Media; Media-Cell Culture Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP