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Add To Compare List EGF elisa kit :: GuineaPig Epidermal growth factor ELISA Kit Catalog # MBS725510 Unit / Price 48-Strip-Wells / $495 +1 FREE 8GB USB 96-Strip-Wells / $700 +1 FREE 8GB USB 5x96-Strip-Wells / $3,105 +3 FREE 8GB USB 10x96-Strip-Wells / $5,865 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Epidermal growth factor (EGF), ELISA Kit Popular Item Full Product Name Guinea Pig Epidermal growth factor ELISA Kit Product Synonym Names Guinea Pig Epidermal growth factor ELISA Kit; Epidermal growth factor; Epidermal growth factor (Guinea Pig) Product Gene Name EGF elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Species Reactivity GuineaPig Specificity This assay has high sensitivity and excellent specificity for detection of EGF. No significant cross-reactivity or interference between EGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EGF and all the analogues, therefore, cross reaction may still exist in some cases. Samples Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Assay Type Quantitative Competitive Sensitivity 0.1 ng/mL Preparation and Storage Store all reagents at 2-8 degree C Sample Preparation We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team ISO Certification Manufactured in an ISO 13485:2016 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of EGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for EGF purchase MBS725510 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Epidermal growth factor (EGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EGF. The ELISA analytical biochemical technique of the MBS725510 kit is based on EGF antibody-EGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for EGF elisa kit Intended Uses: This EGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea PigEGF. This ELISA kit for research use only, not for therapeutic or test applications!Principle of the Assay: EGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-EGF antibody and an EGF-HRP conjugate. The assay sample and buffer are incubated together with EGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EGF concentration since EGF from samples and EGF-HRP conjugate compete for the anti-EGF antibody binding site. Since the number of sites is limited, as more sites are occupied by EGF from the sample, fewer sites are left to bind EGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGF concentration in each sample is interpolated from this standard curve. Product Categories/Family for EGF elisa kit Guinea Pig ELISA Kit Typical Testing Data/Standard Curve (for reference only) of EGF elisa kit TOP Sample Manual Insert of MBS725510. NCBI/Uniprot data below describe general gene information for EGF. It may not necessarily be applicable to this product. NCBI GI # 688072 TOP NCBI Official Full Name epidermal growth factor Protein Family Epidermal growth factor TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with EGF elisa kit Reference Product PubMed Publications FLNA elisa kit >12 publications with EGF and FLNA Pathways associated with EGF elisa kit Products by Pathway Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Organs/Tissues associated with EGF elisa kit Organ/Tissue Name Pubmed Publications Bone Antibodies >13 publications with EGF and Bone Connective Tissue Antibodies >4 publications with EGF and Connective Tissue Heart Antibodies >3 publications with EGF and Heart Muscle Antibodies >3 publications with EGF and Muscle Brain Antibodies >3 publications with EGF and Brain Vascular Antibodies >2 publications with EGF and Vascular Embryonic Tissue Antibodies >2 publications with EGF and Embryonic Tissue Ear Antibodies >1 publications with EGF and Ear Kidney Antibodies >1 publications with EGF and Kidney Umbilical Cord Antibodies >1 publications with EGF and Umbilical Cord TOP