View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List C3a elisa kit :: Mouse Complement fragment 3a, C3a ELISA Kit Catalog # MBS703819 Unit / Price 24-Strip-Wells (LIMIT 1) / $285 +1 FREE 8GB USB 48-Strip-Wells / $555 +1 FREE 8GB USB 96-Strip-Wells / $780 +1 FREE 8GB USB 5x96-Strip-Wells / $2,970 +3 FREE 8GB USB 10x96-Strip-Wells / $5,670 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Complement fragment 3a, C3a, ELISA Kit Popular Item Full Product Name Mouse Complement fragment 3a, C3a ELISA Kit Product Synonym Names Mouse Complement fragment 3a; C3a ELISA Kit; Complement fragment 3a; C3a Product Gene Name C3a elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual MBS703819 Testing Data Testing Data PDF Species Reactivity Mouse Specificity This assay has high sensitivity and excellent specificity for detection of mouse C3a. No significant cross-reactivity or interference between mouse C3a and analogues was observed. Samples Serum, plasma, cell culture supernates, tissue homogenates Assay Type Quantitative Sandwich Detection Range 0.156 ng/ml-10 ng/ml Sensitivity < 0.039 ng/ml Intra-assay Precision Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Preparation and Storage Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions. ISO Certification Manufactured in an ISO 9001:2015 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of C3a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for C3a purchase MBS703819 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement fragment 3a, C3a, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C3a. The ELISA analytical biochemical technique of the MBS703819 kit is based on C3a antibody-C3a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C3a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C3a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Complement fragment 3a C3a is part of the complex complement factor system, which regulates many inflammatory pathways. We have amassed considerable information on complement activation via GPCR mediated signaling but much research is still needed. To aid your assay of serum or plasma of complement C3a proteins, MyBiosource has reagents specifically designed for reactivity to C3a cell samples. We offer mouse and human C3a ELISA kits for your valuable sample preps. Don't miss any protein, use our sandwich format ELISA kit for best results. Each antibody is quality tested and secondary antibodies carefully selected to maximize detection of all C3a antibody bindings, as described in package insert. For WB comparisons, use our anti complement C3a antibody (monoclonal) against C3a human, reactivity human not mouse. View our online catalog for additional complement C3a ELISA kits to different species to expand your models and expand to factors related to complement component 3a. TOP Related Product Information for C3a elisa kit Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C3a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C3a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C3a is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C3a bound in the initial step. The color development is stopped and the intensity of the color is measured. Typical Testing Data/Standard Curve (for reference only) of C3a elisa kit TOP Sample Manual Insert of MBS703819. Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP