View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List WIF1 elisa kit :: Rat WNT Inhibitory Factor 1 ELISA Kit Catalog # MBS048154 Unit / Price 48-Strip-Wells / $560 +1 FREE 8GB USB 96-Strip-Wells / $775 +1 FREE 8GB USB 5x96-Strip-Wells / $3,510 +4 FREE 8GB USB 10x96-Strip-Wells / $6,905 +8 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name WNT Inhibitory Factor 1 (WIF1), ELISA Kit Popular Item Full Product Name Rat WNT Inhibitory Factor 1 ELISA Kit Product Gene Name WIF1 elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Species Reactivity Rat Specificity No significant cross-reactivity or interference between Rat WIF1 and analogues was observed. Samples Serum, Plasma, Tissue Homogenate, Feces and Urine Assay Type Sandwich Detection Range 0.25 ng/ml - 8 ng/ml. Sensitivity 0.1 ng/ml. Intended Uses This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated WIF1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat WIF1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add WIF1 and WIF1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of WIF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision Intra-assay CV (%) is less than 15%. Inter-assay Precision Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100] Preparation and Storage Store all reagents at 2-8 degree C Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of WIF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for WIF1 purchase MBS048154 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the WNT Inhibitory Factor 1 (WIF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WIF1. The ELISA analytical biochemical technique of the MBS048154 kit is based on WIF1 antibody-WIF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WIF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WIF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for WIF1 elisa kit Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated WIF1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat WIF1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add WIF1 and WIF1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of WIF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Sample Manual Insert of MBS048154. Protein Family Wnt inhibitory factor Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. 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