View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List NGB elisa kit :: Rat neuroglobin ELISA Kit Catalog # MBS704958 Unit / Price 24-Strip-Wells (LIMIT 1) / $285 +1 FREE 8GB USB 48-Strip-Wells / $650 +1 FREE 8GB USB 96-Strip-Wells / $930 +1 FREE 8GB USB 5x96-Strip-Wells / $3,370 +4 FREE 8GB USB 10x96-Strip-Wells / $6,395 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name neuroglobin (NGB), ELISA Kit Popular Item Full Product Name Rat Neuroglobin, NGB ELISA Kit Product Synonym Names Rat Neuroglobin; NGB ELISA Kit; neuroglobin Product Gene Name NGB elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual MBS704958 Testing Data Testing Data PDF 3D Structure ModBase 3D Structure for Q6EV97 Species Reactivity Rat Specificity This assay has high sensitivity and excellent specificity for detection of rat NGB. No significant cross-reactivity or interference between rat NGB and analogues was observed. Samples Serum, plasma, cell culture supernates, tissue homogenates Assay Type Sandwich Detection Range 15.6 ng/ml -1000 ng/ml. Sensitivity The minimum detectable dose of rat NGB is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Preparation and Storage Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions. ISO Certification Manufactured in an ISO 9001:2015 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of NGB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for NGB purchase MBS704958 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the neuroglobin (NGB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NGB. The ELISA analytical biochemical technique of the MBS704958 kit is based on NGB antibody-NGB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NGB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NGB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Brain is a major consumer of O2, yet has more limited biodistribution. The work-around? Neuroglobin NGB. This protein binds oxygen with greater affinity than systemic hemoglobin, allowing for increase oxygen concentration. Quantitation by ELISA assay will help determine if human NGB plays a significant pathological role in neurodiseases. Such disease are easily modeled in rat or guinea pig, so a specific neuroglobin ELISA kit is essential. Our ELISA kits are shipped complete and ready for your samples. Results from each plasma or biological fluids sample are obtained in minutes not days using our precision neuroglobin NGB ELISA included reagents - 1 plate 96 tests or wells prebound with mus musculus mouse antibody, labeled secondary antibodies, assay diluent, standard curve protein, wash solution, MBS detection reagent and protocol details - rt datasheet. MyBiosource also offers homo sapiens human NGB ELISA kits, Inquire 96 tests pricing and hu datasheet when ordering. TOP Related Product Information for NGB elisa kit Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGB bound in the initial step. The color development is stopped and the intensity of the color is measured. Typical Testing Data/Standard Curve (for reference only) of NGB elisa kit TOP Sample Manual Insert of MBS704958. NCBI/Uniprot data below describe general gene information for NGB. It may not necessarily be applicable to this product. NCBI GI # 126723275 NCBI GeneID 100008868 NCBI Accession # NP_001075602.1 [Other Products] NCBI GenBank Nucleotide # NM_001082133.2 [Other Products] UniProt Primary Accession # Q6EV97 [Other Products] UniProt Related Accession # Q6EV97[Other Products] Molecular Weight 17,040 Da TOP NCBI Official Full Name neuroglobin NCBI Official Symbol NGB [Similar Products] NCBI Protein Information neuroglobin UniProt Protein Name Neuroglobin Protein Family Neuroglobin UniProt Gene Name NGB [Similar Products] UniProt Entry Name NGB_RABIT TOP TOP UniProt Comments for NGB Function: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes Research Articles on NGB 1. Ngb overexpression may have potential therapeutic benefits for both reducing secondary damages and improving the outcomes after traumatic spinal cord injury TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with NGB elisa kit Reference Product PubMed Publications CYGB elisa kit >26 publications with NGB and CYGB Rac1 elisa kit >1 publications with NGB and Rac1 Pathways associated with NGB elisa kit Products by Pathway Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Diseases associated with NGB elisa kit Disease Name Pubmed Publications Nervous System Diseases Antibodies >54 publications with NGB and Nervous System Diseases Brain Diseases Antibodies >42 publications with NGB and Brain Diseases Cardiovascular Diseases Antibodies >22 publications with NGB and Cardiovascular Diseases Disease Models, Animal Antibodies >18 publications with NGB and Disease Models, Animal Neurodegenerative Diseases Antibodies >16 publications with NGB and Neurodegenerative Diseases Urinary Bladder Diseases Antibodies >11 publications with NGB and Urinary Bladder Diseases Hypertension Antibodies >6 publications with NGB and Hypertension Alzheimer Disease Antibodies >5 publications with NGB and Alzheimer Disease Immune System Diseases Antibodies >4 publications with NGB and Immune System Diseases Congenital Abnormalities Antibodies >4 publications with NGB and Congenital Abnormalities Organs/Tissues associated with NGB elisa kit Organ/Tissue Name Pubmed Publications Bone Antibodies >13 publications with NGB and Bone Connective Tissue Antibodies >4 publications with NGB and Connective Tissue Heart Antibodies >3 publications with NGB and Heart Muscle Antibodies >3 publications with NGB and Muscle Brain Antibodies >3 publications with NGB and Brain Vascular Antibodies >2 publications with NGB and Vascular Embryonic Tissue Antibodies >2 publications with NGB and Embryonic Tissue Ear Antibodies >1 publications with NGB and Ear Kidney Antibodies >1 publications with NGB and Kidney Umbilical Cord Antibodies >1 publications with NGB and Umbilical Cord TOP © 2006-24 MyBioSource.com. 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