View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List placental growth hormone 20K Active Protein Catalog # MBS400052 Unit / Price 0.01 mg / $195 +1 FREE 8GB USB 0.05 mg / $285 +1 FREE 8GB USB 0.1 mg / $380 +1 FREE 8GB USB 0.5 mg / $1,000 +1 FREE 8GB USB 1 mg / $1,700 +2 FREE 8GB USB 5 mg / $6,550 +8 FREE 8GB USB 10 mg / $11,080 +10 FREE 8GB USB 100 mg / $26,525 +10 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name placental growth hormone 20K, Active Protein Popular Item Full Product Name Human placental growth hormone 20K Product Synonym Names Human placental growth hormone 20K Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pr o-Thr-Ile. Host Escherichia Coli Purity/Purification Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 11.(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Form/Format The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 11. Solubility It is recommended to reconstitute the lyophilized rHuGH-20K-pl in 0.4% NaHCO3 or water adjusted to pH 11, not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Protein Content Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.14 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of GH-20K-pl as a Reference Standard. Dimers and Aggregates Less than 3% as determined by silver-stained SDS-PAGE gel analysis. Endotoxin Less than 0.1 ng/ug (IEU/ug) of human GH. Formulation The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 11. Biological Activity PLR'r HuGH-20K is fully biologically active when compared to World Health Organization (WHO) reference standard as evidenced by in vitro bioassay. It is also capable of forming a 1:2 complex with the recombinant human growth hormone receptor extracellular domain (ECD). Preparation and Storage Lyophilized rHuGH-20K-pl although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution and filter sterilization rHuGH-20K-pl can be stored at 4 degree C, pH 11 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Active Protein Note Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product. Other Notes Small volumes of placental growth hormone 20K active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for placental growth hormone 20K active protein Recombinant Placental Human GH-20K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20498 Dalton. Predicted pI=8.20. rHuGH-20K-pl is almost completely devoid of lactogenic (prolactin receptor mediated) activity characteristic to pituitary hGHs.rHuGH-20K-pl is purified by proprietary chromatographic techniques. Product Categories/Family for placental growth hormone 20K active protein Growth Hormones; prolactins; placental lactogens Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP