View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List EcoRI 10u/ul Restriction Enzyme Catalog # MBS638392 Unit / Price 5x5000 Units / $530 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name EcoRI 10u/ul, Restriction Enzyme Full Product Name EcoRI 10u/ul Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP MBS638392 Technical Datasheet Technical Datasheet PDF Purity/Purification Molecular Biology Grade Digestion of Agarose-embedded DNA A minimum of 5u of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends XapI, MunI, TasI Supplied With R1625: Restriction Enzyme Buffer A, 10XR1625-03: Restriction Enzyme Buffer D, 10X Blue/White Cloning Assay pUC57 was digested at a unique site with 10u of enzyme for 16 hours. After religation and transformation 0.2% of white colonies were detected. Lambda 5 Ligation/Recutting Assay After 50-fold overdigestion (3u/ug DNA x 17 hours) with EcoRI, more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 0.02uM. More than 90% of these can be recut. M13mp18/19 1 PhiX174 0 pTZ19R/U 1 pUC18/19 1 pUC57 1 Stability During Prolonged Incubation A minimum of 0.2u of enzyme are required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C. Quality Control Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with EcoRI. Thermal Inactivation Enzyme is inactivated by incubation at 65 degree C for 20min. Unit Definition One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer. Preparation and Storage Store at -20C. Stable for 12-12 months. Other Notes Small volumes of EcoRI 10u/ul restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for EcoRI 10u/ul restriction enzyme 5'-G^A A T T C-3' Product Categories/Family for EcoRI 10u/ul restriction enzyme Molecular Biology; MB-Enzymes; Restriction Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP