View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List IgG (H&L) Secondary Antibody Catalog # MBS686085 Unit / Price 0.5 mg / $230 +1 FREE 8GB USB 5x0.5 mg / $870 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name IgG (H&L), Secondary Antibody Popular Item Full Product Name Chicken anti-Rabbit IgG (H&L) - Affinity Pure, TRITC Conjugate, min x w/human or mouse IgG Product Synonym Names Chicken anti-Rabbit IgG (H&L) - Affinity Pure Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP MBS686085 COA COA PDF Host Chicken Specificity Based on IEP, this antibody reacts with: heavy (gamma) chains on rabbit IgG, light chains on all rabbit immunoglobulins Purity/Purification Affinity purified antibody is > 95% based on SDS-PAGEAffinity purified using solid phase Rabbit IgG. Form/Format Lyophilized; 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free Concentration 0.5 mg of antibody at 1.00 mg/mL(E1% at 280 nm = 13.2) (lot specific) Fluorophore Tetramethylrhodamine-5-isothiocyanate (TRITC) Ex = 550 nm; Em = 570 nm Fluor/Protein Ratio 2.2 Moles TRITC per Mole Antibody Immunogen Purified Rabbit IgG, whole molecule Preservative 0.05% (w/v) Sodium Azide Reconstitution Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily. Cross Reactivity Based on IEP, no reactivity is observed to• non-immunoglobulin Rabbit serum proteins• IgG from Human or Mouse Preparation and Storage Store lyophilized material at 2-8 degree C.For storage ay -20°C after reconstitution, dilute with an equal volume of glycerol to prevent loss of enzymatic activity.Prepare working dilution prior to use and then discard. Quality Manufacturing Systems Manufactured under ISO QSR (Quality System Requirements) guidelines, compliant with ISO 13485:2016. Other Notes Small volumes of IgG (H&L) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Product Categories/Family for IgG (H&L) secondary antibody Secondary Antibodies Applications Tested/Suitable for IgG (H&L) secondary antibody Flow Cytometry (FC), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM), Immunohistochemistry (IHC) Application Notes for IgG (H&L) secondary antibody • 1:20-1:2,000 for most applications.The optimal working dilution should be determined by the investigator. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP