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1, 5 AG elisa kit :: Human 1, 5 Anhydroglucitol ELISA Kit

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Catalog # MBS723128
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

1, 5 Anhydroglucitol (1, 5 AG), ELISA Kit

Popular Item
Also Known As

Human 1, 5 Anhydroglucitol ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
25-500umoL/L
Sensitivity
1.0umoL/L
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of 1, 5 AG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for1, 5 AGpurchase
MBS723128 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1, 5 Anhydroglucitol (1, 5 AG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 1, 5 AG. The ELISA analytical biochemical technique of the MBS723128 kit is based on 1, 5 AG antibody-1, 5 AG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 1, 5 AG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 1, 5 AG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
1, 5 AG elisa kit
For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

Intended Uses: This 1, 5-AG ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of 1, 5-AG in the sample, this 1, 5-AG ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus 1, 5-AG concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of 1, 5-AG utilizes a multiclonal anti-1, 5-AG antibody and an 1, 5-AG-HRP conjugate. The assay sample and buffer are incubated together with 1, 5-AG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 1, 5-AG concentration since 1, 5-AG from samples and 1, 5-AG-HRP conjugate compete for the anti-1, 5-AG antibody binding site. Since the number of sites is limited, as more sites are occupied by 1, 5-AG from the sample, fewer sites are left to bind 1, 5-AG-HRP conjugate. Standards of known 1, 5-AG concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of 1, 5-AG. The 1, 5-AG concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of 1, 5 AG elisa kit
1, 5 AG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS723128. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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