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14-3-3 alpha/beta recombinant protein

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Catalog # MBS515396
Unit / Price
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  0.02 mg  /  $235 +1 FREE 8GB USB
  0.05 mg  /  $365 +1 FREE 8GB USB
SDS-PAGE
Product Name

14-3-3 alpha/beta, Recombinant Protein

Full Product Name

14-3-3 alpha/beta recombinant protein

Product Synonym Names
14-3-3 alpha/beta; YWHAB; HS1; GW128; KCIP-1; 14-3-3alpha/beta Protein; 14-3-3a/b Protein
Product Synonym Gene Name
14-3-3 alpha/beta; YWHAB; HS1; GW128; KCIP-1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 20; NC_000020.10 (43514240..43537173). Location: 20q13.1
OMIM
601289
3D Structure
ModBase 3D Structure for P31946
Host
E Coli
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Type
Recombinant Fusion Protein
Species
Human
Tag Information
tag-free
Expression System
E.coli
Source Note
Recombinant full-length human tag-free 14-3-3alpha/beta was expressed in E. coli cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Other Notes
Small volumes of 14-3-3 alpha/beta recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
14-3-3 alpha/beta recombinant protein
Recombinant full-length human tag-free 14-3-3alpha/beta was expressed in E. coli cells.

Scientific Background: 14-3-3alpha/beta is a member of the highly conserved 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins (1). 14-3-3alpha/beta protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. 14-3-3-beta interacts with the TSC1-TSC2 dimer (2). The interaction required phosphorylation of TSC2 at Ser1210. Binding of 14-3-3-beta to TSC2 did not alter the interaction between TSC1 and TSC2, but it reduced the ability of the complex to inhibit phosphorylation of ribosomal protein S6 kinase impairing the ability of the complex to inhibit cell growth.
Applications Tested/Suitable for 14-3-3 alpha/beta recombinant protein
Western Blot (WB)

NCBI/Uniprot data below describe general gene information for 14-3-3 alpha/beta. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
~29kDa
NCBI Official Full Name
Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB), transcript variant 1, mRNA
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
NCBI Official Symbol
YWHAB  [Similar Products]
NCBI Official Synonym Symbols
HS1; GW128; YWHAA; KCIP-1
  [Similar Products]
NCBI Protein Information
14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide
UniProt Protein Name
14-3-3 protein beta/alpha
UniProt Synonym Protein Names
Protein 1054; Protein kinase C inhibitor protein 1
UniProt Gene Name
YWHAB  [Similar Products]
UniProt Synonym Gene Names
KCIP-1  [Similar Products]
UniProt Entry Name
1433B_HUMAN
NCBI Summary for 14-3-3 alpha/beta
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for 14-3-3 alpha/beta
Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Ref.13 Ref.15

Subunit structure: Homodimer. Interacts with SAMSN1 and PRKCE

By similarity. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2 and YAP1 (phosphorylated form). Interacts with the phosphorylated (by AKT1) form of SRPK2. Interacts with PKA-phosphorylated AANAT. Ref.8 Ref.9 Ref.10 Ref.12 Ref.13 Ref.14 Ref.15 Ref.19

Subcellular location: Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.11

Post-translational modification: The alpha, brain-specific form differs from the beta form in being phosphorylated

By similarity. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion

By similarity. Ref.13Isoform Short contains a N-acetylmethionine at position 1

By similarity.

Sequence similarities: Belongs to the 14-3-3 family.
Product References and Citations for 14-3-3 alpha/beta recombinant protein
1.Yaffe, M B. et al: The structural basis for 14-3-3:phosphopeptide binding specificity. Cell 91: 961-971, 1997.
2.Shumway, S D. et al: 14-3-3-beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin. J. Biol. Chem. 278: 2089-2092, 2003.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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