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14-3-3 beta / YWHAB recombinant protein

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Catalog # MBS2546049
Unit / Price
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  0.05 mg  /  $405 +1 FREE 8GB USB
  0.1 mg  /  $635 +1 FREE 8GB USB
SDS-PAGE
Product Name

14-3-3 beta / YWHAB, Recombinant Protein

Full Product Name

Recombinant Human 14-3-3 beta / YWHAB Protein

Product Synonym Names
GW128, HS1, KCIP-1, YWHAB
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X57346 mRNA
3D Structure
ModBase 3D Structure for P31946
Host
E Coli
Purity/Purification
> 93 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.1mM DTT, 10% glycerol, pH 7.5
Predicted N Terminal
Met
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of 14-3-3 beta / YWHAB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
14-3-3 beta / YWHAB recombinant protein
Background: 14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.

Description: A DNA sequence encoding the human YWHAB (NP_003395.1) (Met 1-Asn 246) was expressed, with two additional amino acids (Gly & Pro) at the N-terminus.
The recombinant human YWHAB consists of 248 amino acids and has a predicted molecular mass of 28 kDa as estimated in SDS-PAGE under reducing conditions.

NCBI/Uniprot data below describe general gene information for 14-3-3 beta / YWHAB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,850 Da
NCBI Official Full Name
14-3-3 protein beta/alpha
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta
NCBI Official Symbol
YWHAB  [Similar Products]
NCBI Official Synonym Symbols
HS1; GW128; YWHAA; KCIP-1; HEL-S-1
  [Similar Products]
NCBI Protein Information
14-3-3 protein beta/alpha
UniProt Protein Name
14-3-3 protein beta/alpha
UniProt Synonym Protein Names
Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1
UniProt Gene Name
YWHAB  [Similar Products]
UniProt Synonym Gene Names
KCIP-1  [Similar Products]
NCBI Summary for 14-3-3 beta / YWHAB
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for 14-3-3 beta / YWHAB
14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell-cell adherens junction; cytoplasm; cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane; perinuclear region of cytoplasm

Molecular Function: enzyme binding; histone deacetylase binding; phosphoprotein binding; phosphoserine binding; protein binding; protein domain specific binding

Biological Process: cytoplasmic sequestering of protein; MAPKKK cascade; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of protein amino acid dephosphorylation; positive regulation of catalytic activity; regulation of mRNA stability
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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