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anti-14-3-3 beta/zeta antibody :: 14-3-3 beta/zeta Antibody

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Catalog # MBS854588
Unit / Price
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  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

14-3-3 beta/zeta, Antibody

Full Product Name

14-3-3 beta/zeta (Phospho-Ser184/186) Antibody

Product Synonym Names
14-3-3 protein zeta/delta; 1433Z; 143Z; Factor activating exoenzyme S; FAS; KCIP-1; Mitochondrial import stimulation factor S1 subunit; Protein kinase C inhibitor protein-1; YWHAZ
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601288
3D Structure
ModBase 3D Structure for P63104
Species Reactivity
Human, Mouse, Rat
Specificity
14-3-3 beta/zeta (Phospho-Ser184/186) antibody detects endogenous levels of 14-3-3 zeta/beta only when phosphorylated at serine 184 or serine 186.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 zeta/beta around the phosphorylation site of serine 184 or serine 186 (L-N-SP-P-E).
All Sites
Human: Ser184/186
Mouse: Ser184/186
Rat: Ser184/186
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-14-3-3 beta/zeta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-14-3-3 beta/zeta antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-14-3-3 beta/zeta antibody
WB: 1:500~1:1000
ELISA: 1:5000

Western Blot (WB) of anti-14-3-3 beta/zeta antibody
Western blot analysis of extracts from RAW264.7 cells, treated with UV (15mins), using 14-3-3 beta/zeta (Phospho-Ser184/186) antibody. 14-3-3 beta/zeta (Phospho-Ser184/186) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.
anti-14-3-3 beta/zeta antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for 14-3-3 beta/zeta. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19,333 Da
NCBI Official Full Name
14-3-3 protein zeta/delta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
NCBI Official Symbol
YWHAZ  [Similar Products]
NCBI Official Synonym Symbols
HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta
  [Similar Products]
NCBI Protein Information
14-3-3 protein zeta/delta
UniProt Protein Name
14-3-3 protein zeta/delta
UniProt Synonym Protein Names
Protein kinase C inhibitor protein 1; KCIP-1
UniProt Gene Name
YWHAZ  [Similar Products]
UniProt Synonym Gene Names
KCIP-1  [Similar Products]
NCBI Summary for 14-3-3 beta/zeta
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]
UniProt Comments for 14-3-3 beta/zeta
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Product References and Citations for anti-14-3-3 beta/zeta antibody
Zupan L.A., J. Biol. Chem. 267:8707-8710(1992). Seluja G.A., Biochim. Biophys. Acta 1395:281-287(1998). The MGC Project Team; Genome Res. 14:2121-2127(2004).

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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