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anti-4E-BP1 antibody :: 4E-BP1 Antibody

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Catalog # MBS8510050
Unit / Price
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  0.1 mg  /  $290 +1 FREE 8GB USB
Product Name

4E-BP1, Antibody

Full Product Name

4E-BP1 (Ab-36) Antibody

Product Synonym Names
4E-BP1; eIF4E-binding protein 1; Phosphorylated heat-and acid-stable protein regulated by insulin 1; PHAS-I
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13541
Species Reactivity
Human, Mouse, Rat
4E-BP1 (Ab-36) antibody detects endogenous levels of total 4E-BP1 protein. This antibody may cross-react with 4E-BP2 and 4E-BP3.
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
The antiserum was produced against synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-TP-P-G).
Preparation and Storage
Store at-20 degree C for 1 year
Other Notes
Small volumes of anti-4E-BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-4E-BP1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-4E-BP1 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:1000

Immunohistochemistry (IHC) of anti-4E-BP1 antibody
Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-36) antibody.
anti-4E-BP1 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-4E-BP1 antibody
Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200ng/ml, 30min), using 4E-BP1 (Ab-36) antibody (Line 1 and 2) and 4E-BP1 (phospho-Thr36) antibody (Line 3 and 4).
anti-4E-BP1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for 4E-BP1. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
12,580 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
EIF4EBP1  [Similar Products]
NCBI Official Synonym Symbols
BP-1; 4EBP1; 4E-BP1; PHAS-I
  [Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Synonym Protein Names
Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
UniProt Gene Name
EIF4EBP1  [Similar Products]
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I  [Similar Products]
NCBI Summary for 4E-BP1
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
UniProt Comments for 4E-BP1
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Product References and Citations for anti-4E-BP1 antibody
Gingras AC, et al. Genes Dev Nov. 2005 Feb 15. Mothe-Satney I, et al.(2000) J Biol Chem:15(21): 2852-64. Gingras AC, et al. (1999) Genes Dev:13(11): 1422-37. Lal L, et al.(2005) Blood; 105(4): 1669-7.

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