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5-LO elisa kit :: Porcine 5-Lipoxygenase ELISA Kit

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Catalog # MBS019597
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
5-LO elisa kit
Product Name

5-Lipoxygenase (5-LO), ELISA Kit

Popular Item
Full Product Name

Porcine 5-Lipoxygenase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 10; NC_000010.10 (45869624..45941567). Location: 10q11.2
OMIM
152390
3D Structure
ModBase 3D Structure for P09917
Species Reactivity
Specificity
No significant cross-reactivity or interference between Porcine 5-LO and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type
Sandwich
Detection Range
31.2 U/L - 1000 U/L.
Sensitivity
5.0 U/L.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 5-LO concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine 5-LO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 5-LO and 5-LO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5-LO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of 5-LO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for5-LOpurchase
MBS019597 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-Lipoxygenase (5-LO) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 5-LO. The ELISA analytical biochemical technique of the MBS019597 kit is based on 5-LO antibody-5-LO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 5-LO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 5-LO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
5-LO elisa kit
Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 5-LO concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine 5-LO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 5-LO and 5-LO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5-LO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS019597. Click to request current manual
NCBI/Uniprot data below describe general gene information for 5-LO. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
77,983 Da
NCBI Official Full Name
5-lipoxygenase
NCBI Official Synonym Full Names
arachidonate 5-lipoxygenase
NCBI Official Symbol
ALOX5  [Similar Products]
NCBI Official Synonym Symbols
5-LO; 5LPG; LOG5; 5-LOX
  [Similar Products]
NCBI Protein Information
arachidonate 5-lipoxygenase; LOX-5; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform
UniProt Protein Name
Arachidonate 5-lipoxygenase
UniProt Gene Name
ALOX5  [Similar Products]
UniProt Synonym Gene Names
LOG5; 5-LO  [Similar Products]
UniProt Entry Name
LOX5_HUMAN
NCBI Summary for 5-LO
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Comments for 5-LO
5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.

Protein type: EC 1.13.11.34; Lipid Metabolism - arachidonic acid; Nuclear envelope; Lipid Metabolism - linoleic acid; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol

Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity

Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Asthma, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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