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anti-53BP1 antibody :: anti-Human 53BP1 Antibody

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Catalog # MBS852894
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

53BP1, Antibody

Also Known As

53BP1 (Ab-1778) Antibody

Product Synonym Names
p53-binding protein 1; P531; TP53B; TP53BP1; TRP53BP1; Tumor suppressor p53-binding protein 1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1975
OMIM
605230
3D Structure
ModBase 3D Structure for Q12888
Species Reactivity
Human
Specificity
53BP1 antibody detects endogenous levels of total 53BP1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized peptide derived from Internal of human 53BP1.
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-53BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-53BP1 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-53BP1 antibody
IHC: 1:50-1:100
ELISA: 1:1000

Immunohistochemistry (IHC) of anti-53BP1 antibody
Peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using 53BP1 antibody.
anti-53BP1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for 53BP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
213,989 Da
NCBI Official Full Name
TP53-binding protein 1 isoform 2
NCBI Official Synonym Full Names
tumor protein p53 binding protein 1
NCBI Official Symbol
TP53BP1  [Similar Products]
NCBI Official Synonym Symbols
TP53; p202; 53BP1; TDRD30; p53BP1
  [Similar Products]
NCBI Protein Information
TP53-binding protein 1
UniProt Protein Name
TP53-binding protein 1
UniProt Gene Name
TP53BP1  [Similar Products]
UniProt Synonym Gene Names
p53BP1  [Similar Products]
NCBI Summary for 53BP1
This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
UniProt Comments for 53BP1
Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates to the repair and the orientation of the broken DNA ends during CSR (). In contrast, it is not required for classic NHEJ and V(D)J recombination (). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).
Product References and Citations for anti-53BP1 antibody
Iwabuchi K., J. Biol. Chem. 273:26061-26068(1998). The German cDNA consortium; Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases. Nakajima D., Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.

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