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5NT elisa kit :: Human 5 Nucleotidase ELISA Kit

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Catalog # MBS723089
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

5 Nucleotidase (5NT), ELISA Kit

Also Known As

Human 5 Nucleotidase ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
gene 211800
3D Structure
ModBase 3D Structure for P21589
Species Reactivity
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Detection Range
Intended Uses
This 5NT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 5NT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of 5NT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for5NTpurchase
MBS723089 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5 Nucleotidase (5NT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 5NT. The ELISA analytical biochemical technique of the MBS723089 kit is based on 5NT antibody-5NT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 5NT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 5NT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
5NT elisa kit
Principle of the Assay: 5NT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-5NT antibody and an 5NT-HRP conjugate. The assay sample and buffer are incubated together with 5NT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 5NT concentration since 5NT from samples and 5NT-HRP conjugate compete for the anti-5NT antibody binding site. Since the number of sites is limited, as more sites are occupied by 5NT from the sample, fewer sites are left to bind 5NT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 5NT concentration in each sample is interpolated from this standard curve.
Product Categories/Family for 5NT elisa kit

Typical Testing Data/Standard Curve (for reference only) of 5NT elisa kit
5NT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS723089. Click to request current manual
NCBI/Uniprot data below describe general gene information for 5NT. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,949 Da
NCBI Official Full Name
NCBI Official Synonym Full Names
5'-nucleotidase, ecto (CD73)
NCBI Official Symbol
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
  [Similar Products]
NCBI Protein Information
5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase
UniProt Protein Name
UniProt Synonym Protein Names
Ecto-5'-nucleotidase; CD_antigen: CD73
UniProt Gene Name
UniProt Synonym Gene Names
NT5; NTE; 5'-NT  [Similar Products]
UniProt Entry Name
NCBI Summary for 5NT
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
UniProt Comments for 5NT
NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein); Membrane protein, GPI anchor; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of inflammatory response; adenosine biosynthetic process; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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