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A GHR elisa kit :: Human Acyl Ghrelin ELISA Kit

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Catalog # MBS744288
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Acyl Ghrelin (A GHR), ELISA Kit

Popular Item
Full Product Name

Human Acyl Ghrelin ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of AGHRL. No significant cross-reactivity or interference between AGHRL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGHRL and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
50-1000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of A GHR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for A GHRpurchase
MBS744288 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acyl Ghrelin (A GHR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing A GHR. The ELISA analytical biochemical technique of the MBS744288 kit is based on A GHR antibody-A GHR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect A GHR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, A GHR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
A GHR elisa kit
Intended Uses: This AGHRL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AGHRL.

Principle of the Assay: AGHRL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGHRL antibody and an AGHRL-HRP conjugate. The assay sample and buffer are incubated together with AGHRL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGHRL concentration since AGHRL from samples and AGHRL-HRP conjugate compete for the anti-AGHRL antibody binding site. Since the number of sites is limited, as more sites are occupied by AGHRL from the sample, fewer sites are left to bind AGHRL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGHRL concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of A GHR elisa kit
A GHR elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS744288. Click to request current manual
NCBI/Uniprot data below describe general gene information for A GHR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
Molecular Weight
12,992 Da
NCBI Official Full Name
ghrelin
UniProt Protein Name
Ghrelin
UniProt Synonym Protein Names
Ghrelin
UniProt Entry Name
E7CCH4_HUMAN
NCBI Summary for A GHR
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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