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A-Glu elisa kit :: Monkey alpha-Glucosidase ELISA Kit

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Catalog # MBS744987
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

alpha-Glucosidase, ELISA Kit

Popular Item
Full Product Name

Monkey alpha-Glucosidase ELISA Kit

Product Synonym Names
Monkey a-Glucosidase ELISA Kit
Product Gene Name
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
606800
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of AGLU. No significant cross-reactivity or interference between AGLU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGLU and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
25-500ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of A-Glu elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for A-Glupurchase
MBS744987 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-Glucosidase, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing A-Glu. The ELISA analytical biochemical technique of the MBS744987 kit is based on A-Glu antibody-A-Glu antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect A-Glu antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, A-Glu. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
A-Glu elisa kit
Intended Uses: This AGLU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey AGLU.

Principle of the Assay: AGLU ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGLU antibody and an AGLU-HRP conjugate. The assay sample and buffer are incubated together with AGLU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGLU concentration since AGLU from samples and AGLU-HRP conjugate compete for the anti-AGLU antibody binding site. Since the number of sites is limited, as more sites are occupied by AGLU from the sample, fewer sites are left to bind AGLU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGLU concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of A-Glu elisa kit
A-Glu elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS744987. Click to request current manual
NCBI/Uniprot data below describe general gene information for A-Glu. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
105,324 Da
NCBI Official Full Name
alpha-glucosidase
NCBI Official Synonym Full Names
glucosidase, alpha; acid
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
lysosomal alpha-glucosidase; acid maltase; aglucosidase alfa
UniProt Protein Name
Lysosomal alpha-glucosidase
UniProt Synonym Protein Names
Acid maltase; Aglucosidase alfaCleaved into the following 2 chains:76 kDa lysosomal alpha-glucosidase; 70 kDa lysosomal alpha-glucosidase
Protein Family
UniProt Gene Name
UniProt Entry Name
LYAG_HUMAN
NCBI Summary for A-Glu
This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for A-Glu
GAA: Essential for the degradation of glygogen to glucose in lysosomes. Defects in GAA are the cause of glycogen storage disease type 2 (GSD2); also called acid alpha-glucosidase (GAA) deficiency or acid maltase deficiency (AMD). GSD2 is a metabolic disorder with a broad clinical spectrum. The severe infantile form, or Pompe disease, presents at birth with massive accumulation of glycogen in muscle, heart and liver. Cardiomyopathy and muscular hypotonia are the cardinal features of this form whose life expectancy is less than two years. The juvenile and adult forms present as limb-girdle muscular dystrophy beginning in the lower limbs. Final outcome depends on respiratory muscle failure. Patients with the adult form can be free of clinical symptoms for most of their life but finally develop a slowly progressive myopathy. Belongs to the glycosyl hydrolase 31 family.

Protein type: Contractile; Hydrolase; Carbohydrate Metabolism - galactose; EC 3.2.1.20; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 17q25.2-q25.3

Cellular Component: membrane; lysosome; lysosomal membrane

Molecular Function: alpha-glucosidase activity; maltase activity; carbohydrate binding

Biological Process: heart morphogenesis; maltose metabolic process; tissue development; glycogen catabolic process; vacuolar sequestering; glucose metabolic process; sucrose metabolic process; locomotory behavior; muscle maintenance; neuromuscular process controlling posture; lysosome organization and biogenesis; diaphragm contraction; neuromuscular process controlling balance; regulation of the force of heart contraction; cardiac muscle contraction

Disease: Glycogen Storage Disease Ii
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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