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anti-A-RAF antibody :: A-RAF Antibody

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Catalog # MBS859189
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
Product Name

A-RAF, Antibody

Popular Item
Also Known As

A-RAF (Phospho-Tyr302) Antibody

Product Synonym Names
A-RAF; A-Raf proto-oncogene serine/threonine-protein kinase; A-raf-1; ARAF; ARAF1; EC 2.7.11.1; PKS; PKS2; Proto-oncogene Pks; Raf; kinase A-Raf
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
609
OMIM
311010
3D Structure
ModBase 3D Structure for P10398
Species Reactivity
Human, Mouse, Rat
Specificity
A-RAF (Phospho-Tyr301/302) antibody detects endogenous levels of A-RAF only when phosphorylated at tyrosine 301/tyrosine 302.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human A-RAF around the phosphorylation site of tyrosine 301/tyrosine 302 (G-Y-YP-W-E).
All Sites
Human: Tyr301/Tyr302
Mouse: Tyr299/Tyr300
Rat: Tyr299/Tyr300
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-A-RAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-A-RAF antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-A-RAF antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000

Testing Data of anti-A-RAF antibody
P-peptide-+
anti-A-RAF antibody Testing Data image
Immunohistochemistry (IHC) of anti-A-RAF antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using A-RAF (Phospho-Tyr301/302) antibody. Western blot analysis of extracts from HeLa cells, treated with PMA (125ng/ml, 30mins), using A-RAF (Phospho-Tyr301/302) antibody.
anti-A-RAF antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-A-RAF antibody
P-peptide-+ Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr301/302) antibody.
anti-A-RAF antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for A-RAF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,904 Da
NCBI Official Full Name
serine/threonine-protein kinase A-Raf isoform 2
NCBI Official Synonym Full Names
A-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
PKS2; A-RAF; ARAF1; RAFA1
  [Similar Products]
NCBI Protein Information
serine/threonine-protein kinase A-Raf
UniProt Protein Name
Serine/threonine-protein kinase A-Raf
UniProt Synonym Protein Names
Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
UniProt Gene Name
UniProt Synonym Gene Names
ARAF1; PKS; PKS2  [Similar Products]
NCBI Summary for A-RAF
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]
UniProt Comments for A-RAF
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
Product References and Citations for anti-A-RAF antibody
Oliver G. Rössler. Am J Physiol Cell Physiol, May 2004; 286: C1118-C1129. Marie A. Bogoyevitch. J. Biol. Chem., Nov 1995; 270: 26303. Weicheng Liu. J. Biol. Chem., Mar 2004; 279: 10167-10175. M Huleihel. Mol. Cell. Biol., Jul 1986; 6: 2655-2662.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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