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anti-A-RAF antibody :: A-RAF Antibody

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Catalog # MBS859594
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

A-RAF, Antibody

Also Known As

A-RAF (Phospho-Ser299) Antibody

Product Synonym Names
A-Raf proto-oncogene serine/threonine-protein kinase; A-raf-1; Proto-oncogene Pks; ARAF; ARAF1; PKS; PKS2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
609
OMIM
311010
3D Structure
ModBase 3D Structure for P10398
Species Reactivity
Human, Mouse, Rat
Specificity
A-RAF (Phospho-Ser299) antibody detects endogenous levels of A-RAF only when phosphorylated at serine 299.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human A-RAF around the phosphorylation site of serine 299 (R-D-SP-G-Y).
All Sites
Human: Ser299
Mouse: Ser297
Rat: Ser297
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-A-RAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-A-RAF antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-A-RAF antibody
IHC: 1:50-1:100
ELISA: 1:1000

Immunohistochemistry (IHC) of anti-A-RAF antibody
P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using A-RAF (Phospho-Ser299) antibody.
anti-A-RAF antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for A-RAF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,904 Da
NCBI Official Full Name
serine/threonine-protein kinase A-Raf isoform 2
NCBI Official Synonym Full Names
A-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
PKS2; A-RAF; ARAF1; RAFA1
  [Similar Products]
NCBI Protein Information
serine/threonine-protein kinase A-Raf
UniProt Protein Name
Serine/threonine-protein kinase A-Raf
UniProt Synonym Protein Names
Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
UniProt Gene Name
UniProt Synonym Gene Names
ARAF1; PKS; PKS2  [Similar Products]
NCBI Summary for A-RAF
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]
UniProt Comments for A-RAF
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
Product References and Citations for anti-A-RAF antibody
Beck T.W., Nucleic Acids Res. 15:595-609(1987). Lee J.-E., Genomics 20:43-55(1994). Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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