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a1BG elisa kit :: Human Alpha-1-B-Glycoprotein (a1BG) ELISA Kit

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Catalog # MBS2022774 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $665 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Alpha-1-B-Glycoprotein (a1BG), ELISA Kit

Also Known As

Alpha-1-B-Glycoprotein (a1BG) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
373
OMIM
138670
3D Structure
ModBase 3D Structure for P04217
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Alpha-1-B-Glycoprotein (a1BG).
No significant cross-reactivity or interference between Alpha-1-B-Glycoprotein (a1BG) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.147ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Alpha-1-B-Glycoprotein (a1BG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Alpha-1-B-Glycoprotein (a1BG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of a1BG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms fora1BGpurchase
MBS2022774 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-1-B-Glycoprotein (a1BG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing a1BG. The ELISA analytical biochemical technique of the MBS2022774 kit is based on a1BG antibody-a1BG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect a1BG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, a1BG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
a1BG elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-B-Glycoprotein (a1BG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-B-Glycoprotein (a1BG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-B-Glycoprotein (a1BG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-B-Glycoprotein (a1BG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for a1BG elisa kit

Typical Testing Data/Standard Curve (for reference only) of a1BG elisa kit
a1BG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2022774. Click to request current manual
NCBI/Uniprot data below describe general gene information for a1BG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,718 Da
NCBI Official Full Name
alpha-1B-glycoprotein
NCBI Official Synonym Full Names
alpha-1-B glycoprotein
NCBI Official Symbol
NCBI Official Synonym Symbols
A1B; ABG; GAB; HYST2477
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NCBI Protein Information
alpha-1B-glycoprotein
UniProt Protein Name
Alpha-1B-glycoprotein
UniProt Synonym Protein Names
Alpha-1-B glycoprotein
Protein Family
UniProt Gene Name
UniProt Entry Name
A1BG_HUMAN
NCBI Summary for a1BG
The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins. [provided by RefSeq, Jul 2008]
UniProt Comments for a1BG
A1BG: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: extracellular space; extracellular region
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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