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A1CF sirna

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Catalog # MBS8202170
Unit / Price
  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
A1CF sirna
Product Name

A1CF, siRNA

Popular Item
Also Known As

A1CF siRNA (Mouse)

Product Synonym Names
ACF; ASP; APOBEC1 complementation factor; APOBEC1-stimulating protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
595
3D Structure
ModBase 3D Structure for Q5YD48
Host
Synthetic
Species Reactivity
Mouse
Specificity
A1CF siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse A1CF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of A1CF sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
A1CF sirna
siRNA to inhibit A1CF expression using RNA interference
Applications Tested/Suitable for A1CF sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for A1CF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,996 Da
NCBI Official Full Name
APOBEC1 complementation factor
NCBI Official Synonym Full Names
APOBEC1 complementation factor
NCBI Official Symbol
NCBI Official Synonym Symbols
ASP; Acf; ACF64; ACF65; 1810073H04Rik
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NCBI Protein Information
APOBEC1 complementation factor
UniProt Protein Name
APOBEC1 complementation factor
UniProt Synonym Protein Names
APOBEC1-stimulating protein
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
A1CF_MOUSE
UniProt Comments for A1CF
ACF: Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; RNA-binding; RNA processing

Cellular Component: nucleoplasm; endoplasmic reticulum; apolipoprotein B mRNA editing enzyme complex; cytoplasm; nuclear heterochromatin; nucleus

Molecular Function: mRNA binding; protein binding; single-stranded RNA binding; nucleic acid binding; RNA binding; nucleotide binding

Biological Process: protein stabilization; cytidine to uridine editing; mRNA modification; mRNA processing
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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